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Cellular Functions of High-Temperature Requirement Factor A4 in Placenta
The expression of High-temperature requirement factor A4 (HtrA4) mRNA is significantly lower in the chorionic villi of patients with recurrent pregnancy loss (RPL) than in the control group. We conducted an investigation into the cellular functions of HtrA4 using the CRISPR/Cas9 system and shRNA-Htr...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252923/ https://www.ncbi.nlm.nih.gov/pubmed/37296580 http://dx.doi.org/10.3390/cells12111459 |
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author | Pei, Chang-Zhu Choi, Bum-Chae Park, Jun-Hyeok Park, Hyo Young Paek, Jinyoung Lee, Kyung-Ju Yun, Bo-Seong Kim, Young Ju Baek, Kwang-Hyun |
author_facet | Pei, Chang-Zhu Choi, Bum-Chae Park, Jun-Hyeok Park, Hyo Young Paek, Jinyoung Lee, Kyung-Ju Yun, Bo-Seong Kim, Young Ju Baek, Kwang-Hyun |
author_sort | Pei, Chang-Zhu |
collection | PubMed |
description | The expression of High-temperature requirement factor A4 (HtrA4) mRNA is significantly lower in the chorionic villi of patients with recurrent pregnancy loss (RPL) than in the control group. We conducted an investigation into the cellular functions of HtrA4 using the CRISPR/Cas9 system and shRNA-HtrA4 to create knockout BeWo cells and HtrA4 knockdown JEG3 cells. Our results indicated that the knockout BeWo cells exhibited reduced capacity for invasion and fusion, but increased levels of proliferation and migration, with a significantly shortened cell cycle compared to wild-type cells. Wild-type BeWo cells highly expressed cell invasion- and fusion-related factors, while knockout BeWo cells highly expressed migration-, proliferation-, and cell cycle-related factors. The shRNA-HtrA4 JEG3 cells showed a decreased capacity for invasion, but an increased capacity for migration, accompanied by a decrease in the expression of cell invasion-related factors and an increase in migration-related factors. Moreover, our ELISA results revealed that the serum HtrA4 level was lower in patients with RPL than in the controls. These findings suggest that HtrA4 depletion may be associated with placental dysfunction. |
format | Online Article Text |
id | pubmed-10252923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102529232023-06-10 Cellular Functions of High-Temperature Requirement Factor A4 in Placenta Pei, Chang-Zhu Choi, Bum-Chae Park, Jun-Hyeok Park, Hyo Young Paek, Jinyoung Lee, Kyung-Ju Yun, Bo-Seong Kim, Young Ju Baek, Kwang-Hyun Cells Article The expression of High-temperature requirement factor A4 (HtrA4) mRNA is significantly lower in the chorionic villi of patients with recurrent pregnancy loss (RPL) than in the control group. We conducted an investigation into the cellular functions of HtrA4 using the CRISPR/Cas9 system and shRNA-HtrA4 to create knockout BeWo cells and HtrA4 knockdown JEG3 cells. Our results indicated that the knockout BeWo cells exhibited reduced capacity for invasion and fusion, but increased levels of proliferation and migration, with a significantly shortened cell cycle compared to wild-type cells. Wild-type BeWo cells highly expressed cell invasion- and fusion-related factors, while knockout BeWo cells highly expressed migration-, proliferation-, and cell cycle-related factors. The shRNA-HtrA4 JEG3 cells showed a decreased capacity for invasion, but an increased capacity for migration, accompanied by a decrease in the expression of cell invasion-related factors and an increase in migration-related factors. Moreover, our ELISA results revealed that the serum HtrA4 level was lower in patients with RPL than in the controls. These findings suggest that HtrA4 depletion may be associated with placental dysfunction. MDPI 2023-05-24 /pmc/articles/PMC10252923/ /pubmed/37296580 http://dx.doi.org/10.3390/cells12111459 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pei, Chang-Zhu Choi, Bum-Chae Park, Jun-Hyeok Park, Hyo Young Paek, Jinyoung Lee, Kyung-Ju Yun, Bo-Seong Kim, Young Ju Baek, Kwang-Hyun Cellular Functions of High-Temperature Requirement Factor A4 in Placenta |
title | Cellular Functions of High-Temperature Requirement Factor A4 in Placenta |
title_full | Cellular Functions of High-Temperature Requirement Factor A4 in Placenta |
title_fullStr | Cellular Functions of High-Temperature Requirement Factor A4 in Placenta |
title_full_unstemmed | Cellular Functions of High-Temperature Requirement Factor A4 in Placenta |
title_short | Cellular Functions of High-Temperature Requirement Factor A4 in Placenta |
title_sort | cellular functions of high-temperature requirement factor a4 in placenta |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252923/ https://www.ncbi.nlm.nih.gov/pubmed/37296580 http://dx.doi.org/10.3390/cells12111459 |
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