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Digested Cinnamon (Cinnamomum verum J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus

Epigenetic changes, host–gut microbiota interactions, and environmental factors contribute to inflammatory bowel disease (IBD) onset and progression. A healthy lifestyle may help to slow down the chronic or remitting/relapsing intestinal tract inflammation characteristic of IBD. In this scenario, th...

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Autores principales: Lonati, Elena, Sala, Gessica, Corbetta, Paolo, Pagliari, Stefania, Cazzaniga, Emanuela, Botto, Laura, Rovellini, Pierangela, Bruni, Ilaria, Palestini, Paola, Bulbarelli, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253083/
https://www.ncbi.nlm.nih.gov/pubmed/37298151
http://dx.doi.org/10.3390/ijms24119201
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author Lonati, Elena
Sala, Gessica
Corbetta, Paolo
Pagliari, Stefania
Cazzaniga, Emanuela
Botto, Laura
Rovellini, Pierangela
Bruni, Ilaria
Palestini, Paola
Bulbarelli, Alessandra
author_facet Lonati, Elena
Sala, Gessica
Corbetta, Paolo
Pagliari, Stefania
Cazzaniga, Emanuela
Botto, Laura
Rovellini, Pierangela
Bruni, Ilaria
Palestini, Paola
Bulbarelli, Alessandra
author_sort Lonati, Elena
collection PubMed
description Epigenetic changes, host–gut microbiota interactions, and environmental factors contribute to inflammatory bowel disease (IBD) onset and progression. A healthy lifestyle may help to slow down the chronic or remitting/relapsing intestinal tract inflammation characteristic of IBD. In this scenario, the employment of a nutritional strategy to prevent the onset or supplement disease therapies included functional food consumption. Its formulation consists of the addition of a phytoextract enriched in bioactive molecules. A good candidate as an ingredient is the Cinnamon verum aqueous extract. Indeed, this extract, subjected to a process of gastrointestinal digestion simulation (INFOGEST), exhibits beneficial antioxidant and anti-inflammatory properties in an in vitro model of the inflamed intestinal barrier. Here, we deepen the study of the mechanisms related to the effect of digested cinnamon extract pre-treatment, showing a correlation between transepithelial electrical resistance (TEER) decrement and alterations in claudin-2 expression under Tumor necrosis factor-α/Interleukin-1β (TNF-α/IL-1) β cytokine administration. Our results show that pre-treatment with cinnamon extract prevents TEER loss by claudin-2 protein level regulation, influencing both gene transcription and autophagy-mediated degradation. Hence, cinnamon polyphenols and their metabolites probably work as mediators in gene regulation and receptor/pathway activation, leading to an adaptive response against renewed insults.
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spelling pubmed-102530832023-06-10 Digested Cinnamon (Cinnamomum verum J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus Lonati, Elena Sala, Gessica Corbetta, Paolo Pagliari, Stefania Cazzaniga, Emanuela Botto, Laura Rovellini, Pierangela Bruni, Ilaria Palestini, Paola Bulbarelli, Alessandra Int J Mol Sci Article Epigenetic changes, host–gut microbiota interactions, and environmental factors contribute to inflammatory bowel disease (IBD) onset and progression. A healthy lifestyle may help to slow down the chronic or remitting/relapsing intestinal tract inflammation characteristic of IBD. In this scenario, the employment of a nutritional strategy to prevent the onset or supplement disease therapies included functional food consumption. Its formulation consists of the addition of a phytoextract enriched in bioactive molecules. A good candidate as an ingredient is the Cinnamon verum aqueous extract. Indeed, this extract, subjected to a process of gastrointestinal digestion simulation (INFOGEST), exhibits beneficial antioxidant and anti-inflammatory properties in an in vitro model of the inflamed intestinal barrier. Here, we deepen the study of the mechanisms related to the effect of digested cinnamon extract pre-treatment, showing a correlation between transepithelial electrical resistance (TEER) decrement and alterations in claudin-2 expression under Tumor necrosis factor-α/Interleukin-1β (TNF-α/IL-1) β cytokine administration. Our results show that pre-treatment with cinnamon extract prevents TEER loss by claudin-2 protein level regulation, influencing both gene transcription and autophagy-mediated degradation. Hence, cinnamon polyphenols and their metabolites probably work as mediators in gene regulation and receptor/pathway activation, leading to an adaptive response against renewed insults. MDPI 2023-05-24 /pmc/articles/PMC10253083/ /pubmed/37298151 http://dx.doi.org/10.3390/ijms24119201 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lonati, Elena
Sala, Gessica
Corbetta, Paolo
Pagliari, Stefania
Cazzaniga, Emanuela
Botto, Laura
Rovellini, Pierangela
Bruni, Ilaria
Palestini, Paola
Bulbarelli, Alessandra
Digested Cinnamon (Cinnamomum verum J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus
title Digested Cinnamon (Cinnamomum verum J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus
title_full Digested Cinnamon (Cinnamomum verum J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus
title_fullStr Digested Cinnamon (Cinnamomum verum J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus
title_full_unstemmed Digested Cinnamon (Cinnamomum verum J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus
title_short Digested Cinnamon (Cinnamomum verum J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus
title_sort digested cinnamon (cinnamomum verum j. presl) bark extract modulates claudin-2 gene expression and protein levels under tnfα/il-1β inflammatory stimulus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253083/
https://www.ncbi.nlm.nih.gov/pubmed/37298151
http://dx.doi.org/10.3390/ijms24119201
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