Cargando…
PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis
Glutathione S-transferases (GSTs) play a crucial role in responding to abiotic stress and are an important target for research on plant stress tolerance mechanisms. Populus euphratica is a promising candidate species for investigating the abiotic tolerance mechanisms in woody plants. In our previous...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253091/ https://www.ncbi.nlm.nih.gov/pubmed/37298311 http://dx.doi.org/10.3390/ijms24119354 |
_version_ | 1785056324750409728 |
---|---|
author | Meng, Huijing Zhao, Jinna Yang, Yanfei Diao, Kehao Zheng, Guangshun Li, Tao Dai, Xinren Li, Jianbo |
author_facet | Meng, Huijing Zhao, Jinna Yang, Yanfei Diao, Kehao Zheng, Guangshun Li, Tao Dai, Xinren Li, Jianbo |
author_sort | Meng, Huijing |
collection | PubMed |
description | Glutathione S-transferases (GSTs) play a crucial role in responding to abiotic stress and are an important target for research on plant stress tolerance mechanisms. Populus euphratica is a promising candidate species for investigating the abiotic tolerance mechanisms in woody plants. In our previous study, PeGSTU58 was identified as being associated with seed salinity tolerance. In the present study, PeGSTU58 was cloned from P. euphratica and functionally characterized. PeGSTU58 encodes a Tau class GST and is located in both the cytoplasm and nucleus. Transgenic Arabidopsis overexpressing PeGSTU58 displayed enhanced tolerance to salt and drought stress. Under salt and drought stress, the transgenic plants exhibited significantly higher activities of antioxidant enzymes, including SOD, POD, CAT, and GST, compared to the wild-type (WT) plants. Additionally, the expression levels of several stress-responsive genes, including DREB2A, COR47, RD22, CYP8D11, and SOD1 were upregulated in PeGSTU58 overexpression lines compared to those in WT Arabidopsis under salt and drought stress conditions. Furthermore, yeast one-hybrid assays and luciferase analysis showed that PebHLH35 can directly bind to the promoter region of PeGSTU58 and activate its expression. These results indicated that PeGSTU58 was involved in salt and drought stress tolerances by maintaining ROS homeostasis, and its expression was positively regulated by PebHLH35. |
format | Online Article Text |
id | pubmed-10253091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102530912023-06-10 PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis Meng, Huijing Zhao, Jinna Yang, Yanfei Diao, Kehao Zheng, Guangshun Li, Tao Dai, Xinren Li, Jianbo Int J Mol Sci Article Glutathione S-transferases (GSTs) play a crucial role in responding to abiotic stress and are an important target for research on plant stress tolerance mechanisms. Populus euphratica is a promising candidate species for investigating the abiotic tolerance mechanisms in woody plants. In our previous study, PeGSTU58 was identified as being associated with seed salinity tolerance. In the present study, PeGSTU58 was cloned from P. euphratica and functionally characterized. PeGSTU58 encodes a Tau class GST and is located in both the cytoplasm and nucleus. Transgenic Arabidopsis overexpressing PeGSTU58 displayed enhanced tolerance to salt and drought stress. Under salt and drought stress, the transgenic plants exhibited significantly higher activities of antioxidant enzymes, including SOD, POD, CAT, and GST, compared to the wild-type (WT) plants. Additionally, the expression levels of several stress-responsive genes, including DREB2A, COR47, RD22, CYP8D11, and SOD1 were upregulated in PeGSTU58 overexpression lines compared to those in WT Arabidopsis under salt and drought stress conditions. Furthermore, yeast one-hybrid assays and luciferase analysis showed that PebHLH35 can directly bind to the promoter region of PeGSTU58 and activate its expression. These results indicated that PeGSTU58 was involved in salt and drought stress tolerances by maintaining ROS homeostasis, and its expression was positively regulated by PebHLH35. MDPI 2023-05-27 /pmc/articles/PMC10253091/ /pubmed/37298311 http://dx.doi.org/10.3390/ijms24119354 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Meng, Huijing Zhao, Jinna Yang, Yanfei Diao, Kehao Zheng, Guangshun Li, Tao Dai, Xinren Li, Jianbo PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis |
title | PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis |
title_full | PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis |
title_fullStr | PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis |
title_full_unstemmed | PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis |
title_short | PeGSTU58, a Glutathione S-Transferase from Populus euphratica, Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis |
title_sort | pegstu58, a glutathione s-transferase from populus euphratica, enhances salt and drought stress tolerance in transgenic arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253091/ https://www.ncbi.nlm.nih.gov/pubmed/37298311 http://dx.doi.org/10.3390/ijms24119354 |
work_keys_str_mv | AT menghuijing pegstu58aglutathionestransferasefrompopuluseuphraticaenhancessaltanddroughtstresstoleranceintransgenicarabidopsis AT zhaojinna pegstu58aglutathionestransferasefrompopuluseuphraticaenhancessaltanddroughtstresstoleranceintransgenicarabidopsis AT yangyanfei pegstu58aglutathionestransferasefrompopuluseuphraticaenhancessaltanddroughtstresstoleranceintransgenicarabidopsis AT diaokehao pegstu58aglutathionestransferasefrompopuluseuphraticaenhancessaltanddroughtstresstoleranceintransgenicarabidopsis AT zhengguangshun pegstu58aglutathionestransferasefrompopuluseuphraticaenhancessaltanddroughtstresstoleranceintransgenicarabidopsis AT litao pegstu58aglutathionestransferasefrompopuluseuphraticaenhancessaltanddroughtstresstoleranceintransgenicarabidopsis AT daixinren pegstu58aglutathionestransferasefrompopuluseuphraticaenhancessaltanddroughtstresstoleranceintransgenicarabidopsis AT lijianbo pegstu58aglutathionestransferasefrompopuluseuphraticaenhancessaltanddroughtstresstoleranceintransgenicarabidopsis |