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The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts

RNA editing is the process of modifying RNA molecules by inserting, deleting, or substituting nucleotides. In flowering plants, RNA editing occurs predominantly in RNAs encoded by the organellar genomes of mitochondria and chloroplasts, and the main type of editing involves the substitution of cytid...

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Autores principales: Ni, Jia, Song, Wenjian, Ali, Nadia Ahmed, Zhang, Yayi, Xing, Jiani, Su, Kexing, Sun, Xingxing, Zhao, Xiaobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253099/
https://www.ncbi.nlm.nih.gov/pubmed/37298153
http://dx.doi.org/10.3390/ijms24119203
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author Ni, Jia
Song, Wenjian
Ali, Nadia Ahmed
Zhang, Yayi
Xing, Jiani
Su, Kexing
Sun, Xingxing
Zhao, Xiaobo
author_facet Ni, Jia
Song, Wenjian
Ali, Nadia Ahmed
Zhang, Yayi
Xing, Jiani
Su, Kexing
Sun, Xingxing
Zhao, Xiaobo
author_sort Ni, Jia
collection PubMed
description RNA editing is the process of modifying RNA molecules by inserting, deleting, or substituting nucleotides. In flowering plants, RNA editing occurs predominantly in RNAs encoded by the organellar genomes of mitochondria and chloroplasts, and the main type of editing involves the substitution of cytidine with uridine at specific sites. Abnormal RNA editing in plants can affect gene expression, organelle function, plant growth, and reproduction. In this study, we report that ATPC1, the gamma subunit of ATP synthase in Arabidopsis chloroplasts, has an unexpected role in the regulation of editing at multiple sites of plastid RNAs. The loss of function of ATPC1 severely arrests chloroplast development, causing a pale-green phenotype and early seedling lethality. Disruption of ATPC1 increases the editing of matK-640, rps12-i-58, atpH-3′UTR-13210, and ycf2-as-91535 sites while decreasing the editing of rpl23-89, rpoA-200, rpoC1-488, and ndhD-2 sites. We further show that ATPC1 participates in RNA editing by interacting with known multiple-site chloroplast RNA editing factors, including MORFs, ORRM1, and OZ1. The transcriptome in the atpc1 mutant is profoundly affected, with a pattern of defective expression of chloroplast development-related genes. These results reveal that the ATP synthase γ subunit ATPC1 is involved in multiple-site RNA editing in Arabidopsis chloroplasts.
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spelling pubmed-102530992023-06-10 The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts Ni, Jia Song, Wenjian Ali, Nadia Ahmed Zhang, Yayi Xing, Jiani Su, Kexing Sun, Xingxing Zhao, Xiaobo Int J Mol Sci Article RNA editing is the process of modifying RNA molecules by inserting, deleting, or substituting nucleotides. In flowering plants, RNA editing occurs predominantly in RNAs encoded by the organellar genomes of mitochondria and chloroplasts, and the main type of editing involves the substitution of cytidine with uridine at specific sites. Abnormal RNA editing in plants can affect gene expression, organelle function, plant growth, and reproduction. In this study, we report that ATPC1, the gamma subunit of ATP synthase in Arabidopsis chloroplasts, has an unexpected role in the regulation of editing at multiple sites of plastid RNAs. The loss of function of ATPC1 severely arrests chloroplast development, causing a pale-green phenotype and early seedling lethality. Disruption of ATPC1 increases the editing of matK-640, rps12-i-58, atpH-3′UTR-13210, and ycf2-as-91535 sites while decreasing the editing of rpl23-89, rpoA-200, rpoC1-488, and ndhD-2 sites. We further show that ATPC1 participates in RNA editing by interacting with known multiple-site chloroplast RNA editing factors, including MORFs, ORRM1, and OZ1. The transcriptome in the atpc1 mutant is profoundly affected, with a pattern of defective expression of chloroplast development-related genes. These results reveal that the ATP synthase γ subunit ATPC1 is involved in multiple-site RNA editing in Arabidopsis chloroplasts. MDPI 2023-05-24 /pmc/articles/PMC10253099/ /pubmed/37298153 http://dx.doi.org/10.3390/ijms24119203 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ni, Jia
Song, Wenjian
Ali, Nadia Ahmed
Zhang, Yayi
Xing, Jiani
Su, Kexing
Sun, Xingxing
Zhao, Xiaobo
The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
title The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
title_full The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
title_fullStr The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
title_full_unstemmed The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
title_short The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
title_sort atp synthase γ subunit atpc1 regulates rna editing in chloroplasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253099/
https://www.ncbi.nlm.nih.gov/pubmed/37298153
http://dx.doi.org/10.3390/ijms24119203
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