Cargando…
The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
RNA editing is the process of modifying RNA molecules by inserting, deleting, or substituting nucleotides. In flowering plants, RNA editing occurs predominantly in RNAs encoded by the organellar genomes of mitochondria and chloroplasts, and the main type of editing involves the substitution of cytid...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253099/ https://www.ncbi.nlm.nih.gov/pubmed/37298153 http://dx.doi.org/10.3390/ijms24119203 |
_version_ | 1785056326638895104 |
---|---|
author | Ni, Jia Song, Wenjian Ali, Nadia Ahmed Zhang, Yayi Xing, Jiani Su, Kexing Sun, Xingxing Zhao, Xiaobo |
author_facet | Ni, Jia Song, Wenjian Ali, Nadia Ahmed Zhang, Yayi Xing, Jiani Su, Kexing Sun, Xingxing Zhao, Xiaobo |
author_sort | Ni, Jia |
collection | PubMed |
description | RNA editing is the process of modifying RNA molecules by inserting, deleting, or substituting nucleotides. In flowering plants, RNA editing occurs predominantly in RNAs encoded by the organellar genomes of mitochondria and chloroplasts, and the main type of editing involves the substitution of cytidine with uridine at specific sites. Abnormal RNA editing in plants can affect gene expression, organelle function, plant growth, and reproduction. In this study, we report that ATPC1, the gamma subunit of ATP synthase in Arabidopsis chloroplasts, has an unexpected role in the regulation of editing at multiple sites of plastid RNAs. The loss of function of ATPC1 severely arrests chloroplast development, causing a pale-green phenotype and early seedling lethality. Disruption of ATPC1 increases the editing of matK-640, rps12-i-58, atpH-3′UTR-13210, and ycf2-as-91535 sites while decreasing the editing of rpl23-89, rpoA-200, rpoC1-488, and ndhD-2 sites. We further show that ATPC1 participates in RNA editing by interacting with known multiple-site chloroplast RNA editing factors, including MORFs, ORRM1, and OZ1. The transcriptome in the atpc1 mutant is profoundly affected, with a pattern of defective expression of chloroplast development-related genes. These results reveal that the ATP synthase γ subunit ATPC1 is involved in multiple-site RNA editing in Arabidopsis chloroplasts. |
format | Online Article Text |
id | pubmed-10253099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102530992023-06-10 The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts Ni, Jia Song, Wenjian Ali, Nadia Ahmed Zhang, Yayi Xing, Jiani Su, Kexing Sun, Xingxing Zhao, Xiaobo Int J Mol Sci Article RNA editing is the process of modifying RNA molecules by inserting, deleting, or substituting nucleotides. In flowering plants, RNA editing occurs predominantly in RNAs encoded by the organellar genomes of mitochondria and chloroplasts, and the main type of editing involves the substitution of cytidine with uridine at specific sites. Abnormal RNA editing in plants can affect gene expression, organelle function, plant growth, and reproduction. In this study, we report that ATPC1, the gamma subunit of ATP synthase in Arabidopsis chloroplasts, has an unexpected role in the regulation of editing at multiple sites of plastid RNAs. The loss of function of ATPC1 severely arrests chloroplast development, causing a pale-green phenotype and early seedling lethality. Disruption of ATPC1 increases the editing of matK-640, rps12-i-58, atpH-3′UTR-13210, and ycf2-as-91535 sites while decreasing the editing of rpl23-89, rpoA-200, rpoC1-488, and ndhD-2 sites. We further show that ATPC1 participates in RNA editing by interacting with known multiple-site chloroplast RNA editing factors, including MORFs, ORRM1, and OZ1. The transcriptome in the atpc1 mutant is profoundly affected, with a pattern of defective expression of chloroplast development-related genes. These results reveal that the ATP synthase γ subunit ATPC1 is involved in multiple-site RNA editing in Arabidopsis chloroplasts. MDPI 2023-05-24 /pmc/articles/PMC10253099/ /pubmed/37298153 http://dx.doi.org/10.3390/ijms24119203 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ni, Jia Song, Wenjian Ali, Nadia Ahmed Zhang, Yayi Xing, Jiani Su, Kexing Sun, Xingxing Zhao, Xiaobo The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts |
title | The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts |
title_full | The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts |
title_fullStr | The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts |
title_full_unstemmed | The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts |
title_short | The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts |
title_sort | atp synthase γ subunit atpc1 regulates rna editing in chloroplasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253099/ https://www.ncbi.nlm.nih.gov/pubmed/37298153 http://dx.doi.org/10.3390/ijms24119203 |
work_keys_str_mv | AT nijia theatpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT songwenjian theatpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT alinadiaahmed theatpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT zhangyayi theatpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT xingjiani theatpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT sukexing theatpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT sunxingxing theatpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT zhaoxiaobo theatpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT nijia atpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT songwenjian atpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT alinadiaahmed atpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT zhangyayi atpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT xingjiani atpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT sukexing atpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT sunxingxing atpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts AT zhaoxiaobo atpsynthasegsubunitatpc1regulatesrnaeditinginchloroplasts |