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Transcriptome Profile of the Rat Adrenal Gland: Parenchymal and Interstitial Cells

The homeostasis of the adrenal gland plays a decisive role in its proper functioning, both in non-stressful conditions and under the influence of various types of stress. This consists of interactions between all types of cells that make up the organ, including parenchymal and interstitial cells. Th...

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Autores principales: Tyczewska, Marianna, Sujka-Kordowska, Patrycja, Szyszka, Marta, Jopek, Karol, Blatkiewicz, Małgorzata, Malendowicz, Ludwik K., Rucinski, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253150/
https://www.ncbi.nlm.nih.gov/pubmed/37298112
http://dx.doi.org/10.3390/ijms24119159
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author Tyczewska, Marianna
Sujka-Kordowska, Patrycja
Szyszka, Marta
Jopek, Karol
Blatkiewicz, Małgorzata
Malendowicz, Ludwik K.
Rucinski, Marcin
author_facet Tyczewska, Marianna
Sujka-Kordowska, Patrycja
Szyszka, Marta
Jopek, Karol
Blatkiewicz, Małgorzata
Malendowicz, Ludwik K.
Rucinski, Marcin
author_sort Tyczewska, Marianna
collection PubMed
description The homeostasis of the adrenal gland plays a decisive role in its proper functioning, both in non-stressful conditions and under the influence of various types of stress. This consists of interactions between all types of cells that make up the organ, including parenchymal and interstitial cells. The amount of available information on this subject in the rat adrenal glands under non-stressful conditions is insufficient; the aim of the research was to determine the expression of marker genes for rat adrenal cells depending on their location. The material for the study consisted of adrenal glands taken from intact adult male rats that were separated into appropriate zones. Transcriptome analysis by means of Affymetrix(®) Rat Gene 2.1 ST Array was used in the study, followed by real-time PCR validation. Expression analysis of interstitial cell marker genes revealed both the amount of expression of these genes and the zone in which they were expressed. The expression of marker genes for fibroblasts was particularly high in the cells of the ZG zone, while the highest expression of specific macrophage genes was observed in the adrenal medulla. The results of this study, especially with regard to interstitial cells, provide a so far undescribed model of marker gene expression of various cells, both in the cortex and medulla of the sexually mature rat adrenal gland. The interdependence between parenchymal and interstitial cells creates a specific microenvironment that is highly heterogeneous within the gland with respect to some of the interstitial cells. This phenomenon most likely depends on the interaction with the differentiated parenchymal cells of the cortex, as well as the medulla of the gland.
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spelling pubmed-102531502023-06-10 Transcriptome Profile of the Rat Adrenal Gland: Parenchymal and Interstitial Cells Tyczewska, Marianna Sujka-Kordowska, Patrycja Szyszka, Marta Jopek, Karol Blatkiewicz, Małgorzata Malendowicz, Ludwik K. Rucinski, Marcin Int J Mol Sci Article The homeostasis of the adrenal gland plays a decisive role in its proper functioning, both in non-stressful conditions and under the influence of various types of stress. This consists of interactions between all types of cells that make up the organ, including parenchymal and interstitial cells. The amount of available information on this subject in the rat adrenal glands under non-stressful conditions is insufficient; the aim of the research was to determine the expression of marker genes for rat adrenal cells depending on their location. The material for the study consisted of adrenal glands taken from intact adult male rats that were separated into appropriate zones. Transcriptome analysis by means of Affymetrix(®) Rat Gene 2.1 ST Array was used in the study, followed by real-time PCR validation. Expression analysis of interstitial cell marker genes revealed both the amount of expression of these genes and the zone in which they were expressed. The expression of marker genes for fibroblasts was particularly high in the cells of the ZG zone, while the highest expression of specific macrophage genes was observed in the adrenal medulla. The results of this study, especially with regard to interstitial cells, provide a so far undescribed model of marker gene expression of various cells, both in the cortex and medulla of the sexually mature rat adrenal gland. The interdependence between parenchymal and interstitial cells creates a specific microenvironment that is highly heterogeneous within the gland with respect to some of the interstitial cells. This phenomenon most likely depends on the interaction with the differentiated parenchymal cells of the cortex, as well as the medulla of the gland. MDPI 2023-05-23 /pmc/articles/PMC10253150/ /pubmed/37298112 http://dx.doi.org/10.3390/ijms24119159 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tyczewska, Marianna
Sujka-Kordowska, Patrycja
Szyszka, Marta
Jopek, Karol
Blatkiewicz, Małgorzata
Malendowicz, Ludwik K.
Rucinski, Marcin
Transcriptome Profile of the Rat Adrenal Gland: Parenchymal and Interstitial Cells
title Transcriptome Profile of the Rat Adrenal Gland: Parenchymal and Interstitial Cells
title_full Transcriptome Profile of the Rat Adrenal Gland: Parenchymal and Interstitial Cells
title_fullStr Transcriptome Profile of the Rat Adrenal Gland: Parenchymal and Interstitial Cells
title_full_unstemmed Transcriptome Profile of the Rat Adrenal Gland: Parenchymal and Interstitial Cells
title_short Transcriptome Profile of the Rat Adrenal Gland: Parenchymal and Interstitial Cells
title_sort transcriptome profile of the rat adrenal gland: parenchymal and interstitial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253150/
https://www.ncbi.nlm.nih.gov/pubmed/37298112
http://dx.doi.org/10.3390/ijms24119159
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