Derivation of trophoblast stem cells from human expanded potential stem cells

Human trophoblast development study has long been limited by the lack of suitable materials. Here we present a detailed protocol for the differentiation of human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and for the subsequent establishment of TSC lines. The hEP...

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Detalles Bibliográficos
Autores principales: Xu, Shao, Wang, Sidong, Tam, Timothy Theodore Ka Ki, Liu, Pengtao, Ruan, Degong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253153/
https://www.ncbi.nlm.nih.gov/pubmed/37300826
http://dx.doi.org/10.1016/j.xpro.2023.102354
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author Xu, Shao
Wang, Sidong
Tam, Timothy Theodore Ka Ki
Liu, Pengtao
Ruan, Degong
author_facet Xu, Shao
Wang, Sidong
Tam, Timothy Theodore Ka Ki
Liu, Pengtao
Ruan, Degong
author_sort Xu, Shao
collection PubMed
description Human trophoblast development study has long been limited by the lack of suitable materials. Here we present a detailed protocol for the differentiation of human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and for the subsequent establishment of TSC lines. The hEPSC-derived TSC lines can be continuously passaged and are functional in further differentiation into syncytiotrophoblasts and extravillous trophoblasts. The hEPSC-TSC system offers a valuable cell source for studying human trophoblast development in pregnancy. For complete details on the use and execution of this protocol, please refer to Gao et al. (2019)(1) and Ruan et al. (2022).(2)
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spelling pubmed-102531532023-06-12 Derivation of trophoblast stem cells from human expanded potential stem cells Xu, Shao Wang, Sidong Tam, Timothy Theodore Ka Ki Liu, Pengtao Ruan, Degong STAR Protoc Protocol Human trophoblast development study has long been limited by the lack of suitable materials. Here we present a detailed protocol for the differentiation of human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and for the subsequent establishment of TSC lines. The hEPSC-derived TSC lines can be continuously passaged and are functional in further differentiation into syncytiotrophoblasts and extravillous trophoblasts. The hEPSC-TSC system offers a valuable cell source for studying human trophoblast development in pregnancy. For complete details on the use and execution of this protocol, please refer to Gao et al. (2019)(1) and Ruan et al. (2022).(2) Elsevier 2023-06-09 /pmc/articles/PMC10253153/ /pubmed/37300826 http://dx.doi.org/10.1016/j.xpro.2023.102354 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xu, Shao
Wang, Sidong
Tam, Timothy Theodore Ka Ki
Liu, Pengtao
Ruan, Degong
Derivation of trophoblast stem cells from human expanded potential stem cells
title Derivation of trophoblast stem cells from human expanded potential stem cells
title_full Derivation of trophoblast stem cells from human expanded potential stem cells
title_fullStr Derivation of trophoblast stem cells from human expanded potential stem cells
title_full_unstemmed Derivation of trophoblast stem cells from human expanded potential stem cells
title_short Derivation of trophoblast stem cells from human expanded potential stem cells
title_sort derivation of trophoblast stem cells from human expanded potential stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253153/
https://www.ncbi.nlm.nih.gov/pubmed/37300826
http://dx.doi.org/10.1016/j.xpro.2023.102354
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