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Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update
Red blood cell (RBC) transfusion is a lifesaving medical procedure that can treat patients with anemia and hemoglobin disorders. However, the shortage of blood supply and risks of transfusion-transmitted infection and immune incompatibility present a challenge for transfusion. The in vitro generatio...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253210/ https://www.ncbi.nlm.nih.gov/pubmed/37296674 http://dx.doi.org/10.3390/cells12111554 |
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author | Lee, Shin-Jeong Jung, Cholomi Oh, Jee Eun Kim, Sangsung Lee, Sangho Lee, Ji Yoon Yoon, Young-sup |
author_facet | Lee, Shin-Jeong Jung, Cholomi Oh, Jee Eun Kim, Sangsung Lee, Sangho Lee, Ji Yoon Yoon, Young-sup |
author_sort | Lee, Shin-Jeong |
collection | PubMed |
description | Red blood cell (RBC) transfusion is a lifesaving medical procedure that can treat patients with anemia and hemoglobin disorders. However, the shortage of blood supply and risks of transfusion-transmitted infection and immune incompatibility present a challenge for transfusion. The in vitro generation of RBCs or erythrocytes holds great promise for transfusion medicine and novel cell-based therapies. While hematopoietic stem cells and progenitors derived from peripheral blood, cord blood, and bone marrow can give rise to erythrocytes, the use of human pluripotent stem cells (hPSCs) has also provided an important opportunity to obtain erythrocytes. These hPSCs include both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). As hESCs carry ethical and political controversies, hiPSCs can be a more universal source for RBC generation. In this review, we first discuss the key concepts and mechanisms of erythropoiesis. Thereafter, we summarize different methodologies to differentiate hPSCs into erythrocytes with an emphasis on the key features of human definitive erythroid lineage cells. Finally, we address the current limitations and future directions of clinical applications using hiPSC-derived erythrocytes. |
format | Online Article Text |
id | pubmed-10253210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102532102023-06-10 Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update Lee, Shin-Jeong Jung, Cholomi Oh, Jee Eun Kim, Sangsung Lee, Sangho Lee, Ji Yoon Yoon, Young-sup Cells Review Red blood cell (RBC) transfusion is a lifesaving medical procedure that can treat patients with anemia and hemoglobin disorders. However, the shortage of blood supply and risks of transfusion-transmitted infection and immune incompatibility present a challenge for transfusion. The in vitro generation of RBCs or erythrocytes holds great promise for transfusion medicine and novel cell-based therapies. While hematopoietic stem cells and progenitors derived from peripheral blood, cord blood, and bone marrow can give rise to erythrocytes, the use of human pluripotent stem cells (hPSCs) has also provided an important opportunity to obtain erythrocytes. These hPSCs include both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). As hESCs carry ethical and political controversies, hiPSCs can be a more universal source for RBC generation. In this review, we first discuss the key concepts and mechanisms of erythropoiesis. Thereafter, we summarize different methodologies to differentiate hPSCs into erythrocytes with an emphasis on the key features of human definitive erythroid lineage cells. Finally, we address the current limitations and future directions of clinical applications using hiPSC-derived erythrocytes. MDPI 2023-06-05 /pmc/articles/PMC10253210/ /pubmed/37296674 http://dx.doi.org/10.3390/cells12111554 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Lee, Shin-Jeong Jung, Cholomi Oh, Jee Eun Kim, Sangsung Lee, Sangho Lee, Ji Yoon Yoon, Young-sup Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update |
title | Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update |
title_full | Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update |
title_fullStr | Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update |
title_full_unstemmed | Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update |
title_short | Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update |
title_sort | generation of red blood cells from human pluripotent stem cells—an update |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253210/ https://www.ncbi.nlm.nih.gov/pubmed/37296674 http://dx.doi.org/10.3390/cells12111554 |
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