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Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update

Red blood cell (RBC) transfusion is a lifesaving medical procedure that can treat patients with anemia and hemoglobin disorders. However, the shortage of blood supply and risks of transfusion-transmitted infection and immune incompatibility present a challenge for transfusion. The in vitro generatio...

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Autores principales: Lee, Shin-Jeong, Jung, Cholomi, Oh, Jee Eun, Kim, Sangsung, Lee, Sangho, Lee, Ji Yoon, Yoon, Young-sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253210/
https://www.ncbi.nlm.nih.gov/pubmed/37296674
http://dx.doi.org/10.3390/cells12111554
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author Lee, Shin-Jeong
Jung, Cholomi
Oh, Jee Eun
Kim, Sangsung
Lee, Sangho
Lee, Ji Yoon
Yoon, Young-sup
author_facet Lee, Shin-Jeong
Jung, Cholomi
Oh, Jee Eun
Kim, Sangsung
Lee, Sangho
Lee, Ji Yoon
Yoon, Young-sup
author_sort Lee, Shin-Jeong
collection PubMed
description Red blood cell (RBC) transfusion is a lifesaving medical procedure that can treat patients with anemia and hemoglobin disorders. However, the shortage of blood supply and risks of transfusion-transmitted infection and immune incompatibility present a challenge for transfusion. The in vitro generation of RBCs or erythrocytes holds great promise for transfusion medicine and novel cell-based therapies. While hematopoietic stem cells and progenitors derived from peripheral blood, cord blood, and bone marrow can give rise to erythrocytes, the use of human pluripotent stem cells (hPSCs) has also provided an important opportunity to obtain erythrocytes. These hPSCs include both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). As hESCs carry ethical and political controversies, hiPSCs can be a more universal source for RBC generation. In this review, we first discuss the key concepts and mechanisms of erythropoiesis. Thereafter, we summarize different methodologies to differentiate hPSCs into erythrocytes with an emphasis on the key features of human definitive erythroid lineage cells. Finally, we address the current limitations and future directions of clinical applications using hiPSC-derived erythrocytes.
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spelling pubmed-102532102023-06-10 Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update Lee, Shin-Jeong Jung, Cholomi Oh, Jee Eun Kim, Sangsung Lee, Sangho Lee, Ji Yoon Yoon, Young-sup Cells Review Red blood cell (RBC) transfusion is a lifesaving medical procedure that can treat patients with anemia and hemoglobin disorders. However, the shortage of blood supply and risks of transfusion-transmitted infection and immune incompatibility present a challenge for transfusion. The in vitro generation of RBCs or erythrocytes holds great promise for transfusion medicine and novel cell-based therapies. While hematopoietic stem cells and progenitors derived from peripheral blood, cord blood, and bone marrow can give rise to erythrocytes, the use of human pluripotent stem cells (hPSCs) has also provided an important opportunity to obtain erythrocytes. These hPSCs include both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). As hESCs carry ethical and political controversies, hiPSCs can be a more universal source for RBC generation. In this review, we first discuss the key concepts and mechanisms of erythropoiesis. Thereafter, we summarize different methodologies to differentiate hPSCs into erythrocytes with an emphasis on the key features of human definitive erythroid lineage cells. Finally, we address the current limitations and future directions of clinical applications using hiPSC-derived erythrocytes. MDPI 2023-06-05 /pmc/articles/PMC10253210/ /pubmed/37296674 http://dx.doi.org/10.3390/cells12111554 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lee, Shin-Jeong
Jung, Cholomi
Oh, Jee Eun
Kim, Sangsung
Lee, Sangho
Lee, Ji Yoon
Yoon, Young-sup
Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update
title Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update
title_full Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update
title_fullStr Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update
title_full_unstemmed Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update
title_short Generation of Red Blood Cells from Human Pluripotent Stem Cells—An Update
title_sort generation of red blood cells from human pluripotent stem cells—an update
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253210/
https://www.ncbi.nlm.nih.gov/pubmed/37296674
http://dx.doi.org/10.3390/cells12111554
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