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Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study

Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ(9)-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannab...

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Autores principales: Anchesi, Ivan, Schepici, Giovanni, Chiricosta, Luigi, Gugliandolo, Agnese, Salamone, Stefano, Caprioglio, Diego, Pollastro, Federica, Mazzon, Emanuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253367/
https://www.ncbi.nlm.nih.gov/pubmed/37298437
http://dx.doi.org/10.3390/ijms24119486
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author Anchesi, Ivan
Schepici, Giovanni
Chiricosta, Luigi
Gugliandolo, Agnese
Salamone, Stefano
Caprioglio, Diego
Pollastro, Federica
Mazzon, Emanuela
author_facet Anchesi, Ivan
Schepici, Giovanni
Chiricosta, Luigi
Gugliandolo, Agnese
Salamone, Stefano
Caprioglio, Diego
Pollastro, Federica
Mazzon, Emanuela
author_sort Anchesi, Ivan
collection PubMed
description Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ(9)-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannabinol (Δ(8)-THC), an isomer of Δ(9)-THC, is a compound for which, to date, there is no evidence of its role in the modulation of synaptic pathways. The aim of our work was to evaluate the effects of Δ(8)-THC on differentiated SH-SY5Y human neuroblastoma cells. Using next generation sequencing (NGS), we investigated whether Δ(8)-THC could modify the transcriptomic profile of genes involved in synapse functions. Our results showed that Δ(8)-THC upregulates the expression of genes involved in the glutamatergic pathway and inhibits gene expression at cholinergic synapses. Conversely, Δ(8)-THC did not modify the transcriptomic profile of genes involved in the GABAergic and dopaminergic pathways.
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spelling pubmed-102533672023-06-10 Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study Anchesi, Ivan Schepici, Giovanni Chiricosta, Luigi Gugliandolo, Agnese Salamone, Stefano Caprioglio, Diego Pollastro, Federica Mazzon, Emanuela Int J Mol Sci Article Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ(9)-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannabinol (Δ(8)-THC), an isomer of Δ(9)-THC, is a compound for which, to date, there is no evidence of its role in the modulation of synaptic pathways. The aim of our work was to evaluate the effects of Δ(8)-THC on differentiated SH-SY5Y human neuroblastoma cells. Using next generation sequencing (NGS), we investigated whether Δ(8)-THC could modify the transcriptomic profile of genes involved in synapse functions. Our results showed that Δ(8)-THC upregulates the expression of genes involved in the glutamatergic pathway and inhibits gene expression at cholinergic synapses. Conversely, Δ(8)-THC did not modify the transcriptomic profile of genes involved in the GABAergic and dopaminergic pathways. MDPI 2023-05-30 /pmc/articles/PMC10253367/ /pubmed/37298437 http://dx.doi.org/10.3390/ijms24119486 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Anchesi, Ivan
Schepici, Giovanni
Chiricosta, Luigi
Gugliandolo, Agnese
Salamone, Stefano
Caprioglio, Diego
Pollastro, Federica
Mazzon, Emanuela
Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
title Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
title_full Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
title_fullStr Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
title_full_unstemmed Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
title_short Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
title_sort δ(8)-thc induces up-regulation of glutamatergic pathway genes in differentiated sh-sy5y: a transcriptomic study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253367/
https://www.ncbi.nlm.nih.gov/pubmed/37298437
http://dx.doi.org/10.3390/ijms24119486
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