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Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ(9)-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannab...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253367/ https://www.ncbi.nlm.nih.gov/pubmed/37298437 http://dx.doi.org/10.3390/ijms24119486 |
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author | Anchesi, Ivan Schepici, Giovanni Chiricosta, Luigi Gugliandolo, Agnese Salamone, Stefano Caprioglio, Diego Pollastro, Federica Mazzon, Emanuela |
author_facet | Anchesi, Ivan Schepici, Giovanni Chiricosta, Luigi Gugliandolo, Agnese Salamone, Stefano Caprioglio, Diego Pollastro, Federica Mazzon, Emanuela |
author_sort | Anchesi, Ivan |
collection | PubMed |
description | Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ(9)-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannabinol (Δ(8)-THC), an isomer of Δ(9)-THC, is a compound for which, to date, there is no evidence of its role in the modulation of synaptic pathways. The aim of our work was to evaluate the effects of Δ(8)-THC on differentiated SH-SY5Y human neuroblastoma cells. Using next generation sequencing (NGS), we investigated whether Δ(8)-THC could modify the transcriptomic profile of genes involved in synapse functions. Our results showed that Δ(8)-THC upregulates the expression of genes involved in the glutamatergic pathway and inhibits gene expression at cholinergic synapses. Conversely, Δ(8)-THC did not modify the transcriptomic profile of genes involved in the GABAergic and dopaminergic pathways. |
format | Online Article Text |
id | pubmed-10253367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102533672023-06-10 Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study Anchesi, Ivan Schepici, Giovanni Chiricosta, Luigi Gugliandolo, Agnese Salamone, Stefano Caprioglio, Diego Pollastro, Federica Mazzon, Emanuela Int J Mol Sci Article Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ(9)-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannabinol (Δ(8)-THC), an isomer of Δ(9)-THC, is a compound for which, to date, there is no evidence of its role in the modulation of synaptic pathways. The aim of our work was to evaluate the effects of Δ(8)-THC on differentiated SH-SY5Y human neuroblastoma cells. Using next generation sequencing (NGS), we investigated whether Δ(8)-THC could modify the transcriptomic profile of genes involved in synapse functions. Our results showed that Δ(8)-THC upregulates the expression of genes involved in the glutamatergic pathway and inhibits gene expression at cholinergic synapses. Conversely, Δ(8)-THC did not modify the transcriptomic profile of genes involved in the GABAergic and dopaminergic pathways. MDPI 2023-05-30 /pmc/articles/PMC10253367/ /pubmed/37298437 http://dx.doi.org/10.3390/ijms24119486 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Anchesi, Ivan Schepici, Giovanni Chiricosta, Luigi Gugliandolo, Agnese Salamone, Stefano Caprioglio, Diego Pollastro, Federica Mazzon, Emanuela Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
title | Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
title_full | Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
title_fullStr | Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
title_full_unstemmed | Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
title_short | Δ(8)-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study |
title_sort | δ(8)-thc induces up-regulation of glutamatergic pathway genes in differentiated sh-sy5y: a transcriptomic study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253367/ https://www.ncbi.nlm.nih.gov/pubmed/37298437 http://dx.doi.org/10.3390/ijms24119486 |
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