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A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1

Coenzyme A (CoA) is a key cellular metabolite which participates in diverse metabolic pathways, regulation of gene expression and the antioxidant defense mechanism. Human NME1 (hNME1), which is a moonlighting protein, was identified as a major CoA-binding protein. Biochemical studies showed that hNM...

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Autores principales: Tossounian, Maria-Armineh, Hristov, Stefan Denchev, Semelak, Jonathan Alexis, Yu, Bess Yi Kun, Baczynska, Maria, Zhao, Yuhan, Estrin, Dario Ariel, Trujillo, Madia, Filonenko, Valeriy, Gouge, Jerome, Gout, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253429/
https://www.ncbi.nlm.nih.gov/pubmed/37298313
http://dx.doi.org/10.3390/ijms24119359
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author Tossounian, Maria-Armineh
Hristov, Stefan Denchev
Semelak, Jonathan Alexis
Yu, Bess Yi Kun
Baczynska, Maria
Zhao, Yuhan
Estrin, Dario Ariel
Trujillo, Madia
Filonenko, Valeriy
Gouge, Jerome
Gout, Ivan
author_facet Tossounian, Maria-Armineh
Hristov, Stefan Denchev
Semelak, Jonathan Alexis
Yu, Bess Yi Kun
Baczynska, Maria
Zhao, Yuhan
Estrin, Dario Ariel
Trujillo, Madia
Filonenko, Valeriy
Gouge, Jerome
Gout, Ivan
author_sort Tossounian, Maria-Armineh
collection PubMed
description Coenzyme A (CoA) is a key cellular metabolite which participates in diverse metabolic pathways, regulation of gene expression and the antioxidant defense mechanism. Human NME1 (hNME1), which is a moonlighting protein, was identified as a major CoA-binding protein. Biochemical studies showed that hNME1 is regulated by CoA through both covalent and non-covalent binding, which leads to a decrease in the hNME1 nucleoside diphosphate kinase (NDPK) activity. In this study, we expanded the knowledge on previous findings by focusing on the non-covalent mode of CoA binding to the hNME1. With X-ray crystallography, we solved the CoA bound structure of hNME1 (hNME1-CoA) and determined the stabilization interactions CoA forms within the nucleotide-binding site of hNME1. A hydrophobic patch stabilizing the CoA adenine ring, while salt bridges and hydrogen bonds stabilizing the phosphate groups of CoA were observed. With molecular dynamics studies, we extended our structural analysis by characterizing the hNME1-CoA structure and elucidating possible orientations of the pantetheine tail, which is absent in the X-ray structure due to its flexibility. Crystallographic studies suggested the involvement of arginine 58 and threonine 94 in mediating specific interactions with CoA. Site-directed mutagenesis and CoA-based affinity purifications showed that arginine 58 mutation to glutamate (R58E) and threonine 94 mutation to aspartate (T94D) prevent hNME1 from binding to CoA. Overall, our results reveal a unique mode by which hNME1 binds CoA, which differs significantly from that of ADP binding: the α- and β-phosphates of CoA are oriented away from the nucleotide-binding site, while 3′-phosphate faces catalytic histidine 118 (H118). The interactions formed by the CoA adenine ring and phosphate groups contribute to the specific mode of CoA binding to hNME1.
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spelling pubmed-102534292023-06-10 A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1 Tossounian, Maria-Armineh Hristov, Stefan Denchev Semelak, Jonathan Alexis Yu, Bess Yi Kun Baczynska, Maria Zhao, Yuhan Estrin, Dario Ariel Trujillo, Madia Filonenko, Valeriy Gouge, Jerome Gout, Ivan Int J Mol Sci Article Coenzyme A (CoA) is a key cellular metabolite which participates in diverse metabolic pathways, regulation of gene expression and the antioxidant defense mechanism. Human NME1 (hNME1), which is a moonlighting protein, was identified as a major CoA-binding protein. Biochemical studies showed that hNME1 is regulated by CoA through both covalent and non-covalent binding, which leads to a decrease in the hNME1 nucleoside diphosphate kinase (NDPK) activity. In this study, we expanded the knowledge on previous findings by focusing on the non-covalent mode of CoA binding to the hNME1. With X-ray crystallography, we solved the CoA bound structure of hNME1 (hNME1-CoA) and determined the stabilization interactions CoA forms within the nucleotide-binding site of hNME1. A hydrophobic patch stabilizing the CoA adenine ring, while salt bridges and hydrogen bonds stabilizing the phosphate groups of CoA were observed. With molecular dynamics studies, we extended our structural analysis by characterizing the hNME1-CoA structure and elucidating possible orientations of the pantetheine tail, which is absent in the X-ray structure due to its flexibility. Crystallographic studies suggested the involvement of arginine 58 and threonine 94 in mediating specific interactions with CoA. Site-directed mutagenesis and CoA-based affinity purifications showed that arginine 58 mutation to glutamate (R58E) and threonine 94 mutation to aspartate (T94D) prevent hNME1 from binding to CoA. Overall, our results reveal a unique mode by which hNME1 binds CoA, which differs significantly from that of ADP binding: the α- and β-phosphates of CoA are oriented away from the nucleotide-binding site, while 3′-phosphate faces catalytic histidine 118 (H118). The interactions formed by the CoA adenine ring and phosphate groups contribute to the specific mode of CoA binding to hNME1. MDPI 2023-05-27 /pmc/articles/PMC10253429/ /pubmed/37298313 http://dx.doi.org/10.3390/ijms24119359 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tossounian, Maria-Armineh
Hristov, Stefan Denchev
Semelak, Jonathan Alexis
Yu, Bess Yi Kun
Baczynska, Maria
Zhao, Yuhan
Estrin, Dario Ariel
Trujillo, Madia
Filonenko, Valeriy
Gouge, Jerome
Gout, Ivan
A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1
title A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1
title_full A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1
title_fullStr A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1
title_full_unstemmed A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1
title_short A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1
title_sort unique mode of coenzyme a binding to the nucleotide binding pocket of human metastasis suppressor nme1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253429/
https://www.ncbi.nlm.nih.gov/pubmed/37298313
http://dx.doi.org/10.3390/ijms24119359
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