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Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death
Ex vivo hypothermic machine perfusion (HMP) is a strategy for controlling ischemia-reperfusion injury in donation after circulatory death (DCD) liver transplantation. The pH of blood increases with a decrease in temperature and water dissociation, leading to a decrease in [H(+)]. This study aimed to...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253457/ https://www.ncbi.nlm.nih.gov/pubmed/37298042 http://dx.doi.org/10.3390/jcm12113845 |
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author | Sakamoto, Sodai Bochimoto, Hiroki Shibata, Kengo Zin, Nur Khatijah Mohd Fukai, Moto Nakamura, Kosei Ishikawa, Takahisa Fujiyoshi, Masato Shimamura, Tsuyoshi Taketomi, Akinobu |
author_facet | Sakamoto, Sodai Bochimoto, Hiroki Shibata, Kengo Zin, Nur Khatijah Mohd Fukai, Moto Nakamura, Kosei Ishikawa, Takahisa Fujiyoshi, Masato Shimamura, Tsuyoshi Taketomi, Akinobu |
author_sort | Sakamoto, Sodai |
collection | PubMed |
description | Ex vivo hypothermic machine perfusion (HMP) is a strategy for controlling ischemia-reperfusion injury in donation after circulatory death (DCD) liver transplantation. The pH of blood increases with a decrease in temperature and water dissociation, leading to a decrease in [H(+)]. This study aimed to verify the optimal pH of HMP for DCD livers. Rat livers were retrieved 30 min post-cardiac arrest and subjected to 3-h cold storage (CS) in UW solution (CS group) or HMP with UW-gluconate solution (machine perfusion [MP] group) of pH 7.4 (original), 7.6, 7.8, and 8.0 (MP-pH 7.6, 7.8, 8.0 groups, respectively) at 7–10 °C. The livers were subjected to normothermic perfusion to simulate reperfusion after HMP. All HMP groups showed greater graft protection compared to the CS group due to the lower levels of liver enzymes in the former. The MP-pH 7.8 group showed significant protection, evidenced by bile production, diminished tissue injury, and reduced flavin mononucleotide leakage, and further analysis by scanning electron microscopy revealed a well-preserved structure of the mitochondrial cristae. Therefore, the optimum pH of 7.8 enhanced the protective effect of HMP by preserving the structure and function of the mitochondria, leading to reduced reperfusion injury in the DCD liver. |
format | Online Article Text |
id | pubmed-10253457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102534572023-06-10 Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death Sakamoto, Sodai Bochimoto, Hiroki Shibata, Kengo Zin, Nur Khatijah Mohd Fukai, Moto Nakamura, Kosei Ishikawa, Takahisa Fujiyoshi, Masato Shimamura, Tsuyoshi Taketomi, Akinobu J Clin Med Article Ex vivo hypothermic machine perfusion (HMP) is a strategy for controlling ischemia-reperfusion injury in donation after circulatory death (DCD) liver transplantation. The pH of blood increases with a decrease in temperature and water dissociation, leading to a decrease in [H(+)]. This study aimed to verify the optimal pH of HMP for DCD livers. Rat livers were retrieved 30 min post-cardiac arrest and subjected to 3-h cold storage (CS) in UW solution (CS group) or HMP with UW-gluconate solution (machine perfusion [MP] group) of pH 7.4 (original), 7.6, 7.8, and 8.0 (MP-pH 7.6, 7.8, 8.0 groups, respectively) at 7–10 °C. The livers were subjected to normothermic perfusion to simulate reperfusion after HMP. All HMP groups showed greater graft protection compared to the CS group due to the lower levels of liver enzymes in the former. The MP-pH 7.8 group showed significant protection, evidenced by bile production, diminished tissue injury, and reduced flavin mononucleotide leakage, and further analysis by scanning electron microscopy revealed a well-preserved structure of the mitochondrial cristae. Therefore, the optimum pH of 7.8 enhanced the protective effect of HMP by preserving the structure and function of the mitochondria, leading to reduced reperfusion injury in the DCD liver. MDPI 2023-06-04 /pmc/articles/PMC10253457/ /pubmed/37298042 http://dx.doi.org/10.3390/jcm12113845 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sakamoto, Sodai Bochimoto, Hiroki Shibata, Kengo Zin, Nur Khatijah Mohd Fukai, Moto Nakamura, Kosei Ishikawa, Takahisa Fujiyoshi, Masato Shimamura, Tsuyoshi Taketomi, Akinobu Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death |
title | Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death |
title_full | Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death |
title_fullStr | Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death |
title_full_unstemmed | Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death |
title_short | Exploration of Optimal pH in Hypothermic Machine Perfusion for Rat Liver Grafts Retrieved after Circulatory Death |
title_sort | exploration of optimal ph in hypothermic machine perfusion for rat liver grafts retrieved after circulatory death |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253457/ https://www.ncbi.nlm.nih.gov/pubmed/37298042 http://dx.doi.org/10.3390/jcm12113845 |
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