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Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review
This scoping review summarizes what is known about kidney metabolism during hypothermic perfusion preservation. Papers studying kidney metabolism during hypothermic (<12 °C) perfusion were identified (PubMed, Embase, Web of Science, Cochrane). Out of 14,335 initially identified records, 52 were i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253488/ https://www.ncbi.nlm.nih.gov/pubmed/37297808 http://dx.doi.org/10.3390/jcm12113613 |
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author | Verstraeten, Laurence Den abt, Rutger Ghesquière, Bart Jochmans, Ina |
author_facet | Verstraeten, Laurence Den abt, Rutger Ghesquière, Bart Jochmans, Ina |
author_sort | Verstraeten, Laurence |
collection | PubMed |
description | This scoping review summarizes what is known about kidney metabolism during hypothermic perfusion preservation. Papers studying kidney metabolism during hypothermic (<12 °C) perfusion were identified (PubMed, Embase, Web of Science, Cochrane). Out of 14,335 initially identified records, 52 were included [dog (26/52), rabbit (2/52), pig (20/52), human (7/52)]. These were published between 1970–2023, partially explaining study heterogeneity. There is a considerable risk of bias in the reported studies. Studies used different perfusates, oxygenation levels, kidney injury levels, and devices and reported on perfusate and tissue metabolites. In 11 papers, (non)radioactively labeled metabolites (tracers) were used to study metabolic pathways. Together these studies show that kidneys are metabolically active during hypothermic perfusion, regardless of the perfusion setting. Although tracers give us more insight into active metabolic pathways, kidney metabolism during hypothermic perfusion is incompletely understood. Metabolism is influenced by perfusate composition, oxygenation levels, and likely also by pre-existing ischemic injury. In the modern era, with increasing donations after circulatory death and the emergence of hypothermic oxygenated perfusion, the focus should be on understanding metabolic perturbations caused by pre-existing injury levels and the effect of perfusate oxygen levels. The use of tracers is indispensable to understanding the kidney’s metabolism during perfusion, given the complexity of interactions between different metabolites. |
format | Online Article Text |
id | pubmed-10253488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102534882023-06-10 Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review Verstraeten, Laurence Den abt, Rutger Ghesquière, Bart Jochmans, Ina J Clin Med Systematic Review This scoping review summarizes what is known about kidney metabolism during hypothermic perfusion preservation. Papers studying kidney metabolism during hypothermic (<12 °C) perfusion were identified (PubMed, Embase, Web of Science, Cochrane). Out of 14,335 initially identified records, 52 were included [dog (26/52), rabbit (2/52), pig (20/52), human (7/52)]. These were published between 1970–2023, partially explaining study heterogeneity. There is a considerable risk of bias in the reported studies. Studies used different perfusates, oxygenation levels, kidney injury levels, and devices and reported on perfusate and tissue metabolites. In 11 papers, (non)radioactively labeled metabolites (tracers) were used to study metabolic pathways. Together these studies show that kidneys are metabolically active during hypothermic perfusion, regardless of the perfusion setting. Although tracers give us more insight into active metabolic pathways, kidney metabolism during hypothermic perfusion is incompletely understood. Metabolism is influenced by perfusate composition, oxygenation levels, and likely also by pre-existing ischemic injury. In the modern era, with increasing donations after circulatory death and the emergence of hypothermic oxygenated perfusion, the focus should be on understanding metabolic perturbations caused by pre-existing injury levels and the effect of perfusate oxygen levels. The use of tracers is indispensable to understanding the kidney’s metabolism during perfusion, given the complexity of interactions between different metabolites. MDPI 2023-05-23 /pmc/articles/PMC10253488/ /pubmed/37297808 http://dx.doi.org/10.3390/jcm12113613 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Systematic Review Verstraeten, Laurence Den abt, Rutger Ghesquière, Bart Jochmans, Ina Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review |
title | Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review |
title_full | Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review |
title_fullStr | Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review |
title_full_unstemmed | Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review |
title_short | Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review |
title_sort | current insights into the metabolome during hypothermic kidney perfusion—a scoping review |
topic | Systematic Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253488/ https://www.ncbi.nlm.nih.gov/pubmed/37297808 http://dx.doi.org/10.3390/jcm12113613 |
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