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Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks

Apple (Malus × domestica Borkh.) is one of the most cultivated fruit crops in China. Apple trees frequently encounter waterlogging stress, mainly due to excess rainfall, soil compaction, or poor soil drainage, results in yellowing leaves and declined fruit quality and yield in some regions. However,...

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Autores principales: Zhang, Kunxi, Chen, Xiaofei, Yuan, Penghao, Song, Chunhui, Song, Shangwei, Jiao, Jian, Wang, Miaomiao, Hao, Pengbo, Zheng, Xianbo, Bai, Tuanhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253570/
https://www.ncbi.nlm.nih.gov/pubmed/37298249
http://dx.doi.org/10.3390/ijms24119298
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author Zhang, Kunxi
Chen, Xiaofei
Yuan, Penghao
Song, Chunhui
Song, Shangwei
Jiao, Jian
Wang, Miaomiao
Hao, Pengbo
Zheng, Xianbo
Bai, Tuanhui
author_facet Zhang, Kunxi
Chen, Xiaofei
Yuan, Penghao
Song, Chunhui
Song, Shangwei
Jiao, Jian
Wang, Miaomiao
Hao, Pengbo
Zheng, Xianbo
Bai, Tuanhui
author_sort Zhang, Kunxi
collection PubMed
description Apple (Malus × domestica Borkh.) is one of the most cultivated fruit crops in China. Apple trees frequently encounter waterlogging stress, mainly due to excess rainfall, soil compaction, or poor soil drainage, results in yellowing leaves and declined fruit quality and yield in some regions. However, the mechanism underlying the response to waterlogging has not been well elucidated. Therefore, we performed a physiological and transcriptomic analysis to examine the differential responses of two apple rootstocks (waterlogging-tolerant M. hupehensis and waterlogging-sensitive M. toringoides) to waterlogging stress. The results showed that M. toringoides displayed more severe leaf chlorosis during the waterlogging treatment than M. hupehensis. Compared with M. hupehensis, the more severe leaf chlorosis induced by waterlogging stress in M. toringoides was highly correlated with increased electrolyte leakage and superoxide radicals, hydrogen peroxide accumulation, and increased stomata closure. Interestingly, M. toringoides also conveyed a higher ethylene production under waterlogging stress. Furthermore, RNA-seq revealed that a total of 13,913 common differentially expressed genes (DEGs) were differentially regulated between M. hupehensis and M. toringoides under waterlogging stress, especially those DEGs involved in the biosynthesis of flavonoids and hormone signaling. This suggests a possible link of flavonoids and hormone signaling to waterlogging tolerance. Taken together, our data provide the targeted genes for further investigation of the functions, as well as for future molecular breeding of waterlogging-tolerant apple rootstocks.
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spelling pubmed-102535702023-06-10 Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks Zhang, Kunxi Chen, Xiaofei Yuan, Penghao Song, Chunhui Song, Shangwei Jiao, Jian Wang, Miaomiao Hao, Pengbo Zheng, Xianbo Bai, Tuanhui Int J Mol Sci Article Apple (Malus × domestica Borkh.) is one of the most cultivated fruit crops in China. Apple trees frequently encounter waterlogging stress, mainly due to excess rainfall, soil compaction, or poor soil drainage, results in yellowing leaves and declined fruit quality and yield in some regions. However, the mechanism underlying the response to waterlogging has not been well elucidated. Therefore, we performed a physiological and transcriptomic analysis to examine the differential responses of two apple rootstocks (waterlogging-tolerant M. hupehensis and waterlogging-sensitive M. toringoides) to waterlogging stress. The results showed that M. toringoides displayed more severe leaf chlorosis during the waterlogging treatment than M. hupehensis. Compared with M. hupehensis, the more severe leaf chlorosis induced by waterlogging stress in M. toringoides was highly correlated with increased electrolyte leakage and superoxide radicals, hydrogen peroxide accumulation, and increased stomata closure. Interestingly, M. toringoides also conveyed a higher ethylene production under waterlogging stress. Furthermore, RNA-seq revealed that a total of 13,913 common differentially expressed genes (DEGs) were differentially regulated between M. hupehensis and M. toringoides under waterlogging stress, especially those DEGs involved in the biosynthesis of flavonoids and hormone signaling. This suggests a possible link of flavonoids and hormone signaling to waterlogging tolerance. Taken together, our data provide the targeted genes for further investigation of the functions, as well as for future molecular breeding of waterlogging-tolerant apple rootstocks. MDPI 2023-05-26 /pmc/articles/PMC10253570/ /pubmed/37298249 http://dx.doi.org/10.3390/ijms24119298 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Kunxi
Chen, Xiaofei
Yuan, Penghao
Song, Chunhui
Song, Shangwei
Jiao, Jian
Wang, Miaomiao
Hao, Pengbo
Zheng, Xianbo
Bai, Tuanhui
Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks
title Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks
title_full Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks
title_fullStr Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks
title_full_unstemmed Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks
title_short Comparative Physiological and Transcriptome Analysis Reveals Potential Pathways and Specific Genes Involved in Waterlogging Tolerance in Apple Rootstocks
title_sort comparative physiological and transcriptome analysis reveals potential pathways and specific genes involved in waterlogging tolerance in apple rootstocks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253570/
https://www.ncbi.nlm.nih.gov/pubmed/37298249
http://dx.doi.org/10.3390/ijms24119298
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