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Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation
High-performance layer electrochromatography (HPLEC) combines the advantages of overpressured-layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) while overcoming some of their limitations. HPLEC equipment can work in various HPLEC, OPLC, and PPEC modes. The equipment ena...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253573/ https://www.ncbi.nlm.nih.gov/pubmed/37298340 http://dx.doi.org/10.3390/ijms24119389 |
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author | Gwarda, Radosław Łukasz Dzido, Tadeusz Henryk |
author_facet | Gwarda, Radosław Łukasz Dzido, Tadeusz Henryk |
author_sort | Gwarda, Radosław Łukasz |
collection | PubMed |
description | High-performance layer electrochromatography (HPLEC) combines the advantages of overpressured-layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) while overcoming some of their limitations. HPLEC equipment can work in various HPLEC, OPLC, and PPEC modes. The equipment enables HPLEC analysis also with an electroosmotic effect directed against the hydrodynamic flow of the mobile phase. The change in the electric field direction in the separation system does not result in a change in either the direction of the mobile phase flow or the direction of solute migration. The hydrodynamic flow generated by the pump dominates the electroosmotic effect and enables separation against the direction of the latter. Reversed-polarization HPLEC may be advantageous for the analysis of anionic compounds, as it facilitates faster and more selective separation than OPLC performed in similar conditions. This separation mode provides a new possibility to develop and optimize separation methods by performing separation against the electroosmotic effect and without need of any modification of the adsorbent surface. A drawback of this separation mode is the increase in the backpressure at the mobile phase inlet and the limitation of the mobile phase flow rate. Currently, contrary to the single-channel mode, multi-channel reverse-polarity HPLEC still requires some technical and methodological improvements. |
format | Online Article Text |
id | pubmed-10253573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102535732023-06-10 Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation Gwarda, Radosław Łukasz Dzido, Tadeusz Henryk Int J Mol Sci Communication High-performance layer electrochromatography (HPLEC) combines the advantages of overpressured-layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) while overcoming some of their limitations. HPLEC equipment can work in various HPLEC, OPLC, and PPEC modes. The equipment enables HPLEC analysis also with an electroosmotic effect directed against the hydrodynamic flow of the mobile phase. The change in the electric field direction in the separation system does not result in a change in either the direction of the mobile phase flow or the direction of solute migration. The hydrodynamic flow generated by the pump dominates the electroosmotic effect and enables separation against the direction of the latter. Reversed-polarization HPLEC may be advantageous for the analysis of anionic compounds, as it facilitates faster and more selective separation than OPLC performed in similar conditions. This separation mode provides a new possibility to develop and optimize separation methods by performing separation against the electroosmotic effect and without need of any modification of the adsorbent surface. A drawback of this separation mode is the increase in the backpressure at the mobile phase inlet and the limitation of the mobile phase flow rate. Currently, contrary to the single-channel mode, multi-channel reverse-polarity HPLEC still requires some technical and methodological improvements. MDPI 2023-05-28 /pmc/articles/PMC10253573/ /pubmed/37298340 http://dx.doi.org/10.3390/ijms24119389 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Gwarda, Radosław Łukasz Dzido, Tadeusz Henryk Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation |
title | Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation |
title_full | Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation |
title_fullStr | Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation |
title_full_unstemmed | Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation |
title_short | Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation |
title_sort | reverse-polarization high-performance layer electrochromatography—a new approach to anion separation |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253573/ https://www.ncbi.nlm.nih.gov/pubmed/37298340 http://dx.doi.org/10.3390/ijms24119389 |
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