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Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation

High-performance layer electrochromatography (HPLEC) combines the advantages of overpressured-layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) while overcoming some of their limitations. HPLEC equipment can work in various HPLEC, OPLC, and PPEC modes. The equipment ena...

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Autores principales: Gwarda, Radosław Łukasz, Dzido, Tadeusz Henryk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253573/
https://www.ncbi.nlm.nih.gov/pubmed/37298340
http://dx.doi.org/10.3390/ijms24119389
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author Gwarda, Radosław Łukasz
Dzido, Tadeusz Henryk
author_facet Gwarda, Radosław Łukasz
Dzido, Tadeusz Henryk
author_sort Gwarda, Radosław Łukasz
collection PubMed
description High-performance layer electrochromatography (HPLEC) combines the advantages of overpressured-layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) while overcoming some of their limitations. HPLEC equipment can work in various HPLEC, OPLC, and PPEC modes. The equipment enables HPLEC analysis also with an electroosmotic effect directed against the hydrodynamic flow of the mobile phase. The change in the electric field direction in the separation system does not result in a change in either the direction of the mobile phase flow or the direction of solute migration. The hydrodynamic flow generated by the pump dominates the electroosmotic effect and enables separation against the direction of the latter. Reversed-polarization HPLEC may be advantageous for the analysis of anionic compounds, as it facilitates faster and more selective separation than OPLC performed in similar conditions. This separation mode provides a new possibility to develop and optimize separation methods by performing separation against the electroosmotic effect and without need of any modification of the adsorbent surface. A drawback of this separation mode is the increase in the backpressure at the mobile phase inlet and the limitation of the mobile phase flow rate. Currently, contrary to the single-channel mode, multi-channel reverse-polarity HPLEC still requires some technical and methodological improvements.
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spelling pubmed-102535732023-06-10 Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation Gwarda, Radosław Łukasz Dzido, Tadeusz Henryk Int J Mol Sci Communication High-performance layer electrochromatography (HPLEC) combines the advantages of overpressured-layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) while overcoming some of their limitations. HPLEC equipment can work in various HPLEC, OPLC, and PPEC modes. The equipment enables HPLEC analysis also with an electroosmotic effect directed against the hydrodynamic flow of the mobile phase. The change in the electric field direction in the separation system does not result in a change in either the direction of the mobile phase flow or the direction of solute migration. The hydrodynamic flow generated by the pump dominates the electroosmotic effect and enables separation against the direction of the latter. Reversed-polarization HPLEC may be advantageous for the analysis of anionic compounds, as it facilitates faster and more selective separation than OPLC performed in similar conditions. This separation mode provides a new possibility to develop and optimize separation methods by performing separation against the electroosmotic effect and without need of any modification of the adsorbent surface. A drawback of this separation mode is the increase in the backpressure at the mobile phase inlet and the limitation of the mobile phase flow rate. Currently, contrary to the single-channel mode, multi-channel reverse-polarity HPLEC still requires some technical and methodological improvements. MDPI 2023-05-28 /pmc/articles/PMC10253573/ /pubmed/37298340 http://dx.doi.org/10.3390/ijms24119389 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gwarda, Radosław Łukasz
Dzido, Tadeusz Henryk
Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation
title Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation
title_full Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation
title_fullStr Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation
title_full_unstemmed Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation
title_short Reverse-Polarization High-Performance Layer Electrochromatography—A New Approach to Anion Separation
title_sort reverse-polarization high-performance layer electrochromatography—a new approach to anion separation
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253573/
https://www.ncbi.nlm.nih.gov/pubmed/37298340
http://dx.doi.org/10.3390/ijms24119389
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