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Oral Microbial Signature of Rheumatoid Arthritis in Female Patients

This study aimed to identify the oral microbial signature of Kazakh female rheumatoid arthritis (RA) patients. A total of 75 female patients who met the American College of Rheumatology 2010 classification criteria for RA and 114 healthy volunteers were included in the study. Amplicons of the 16S rR...

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Autores principales: Kozhakhmetov, Samat, Babenko, Dmitriy, Issilbayeva, Argul, Nurgaziyev, Madiyar, Kozhakhmetova, Saniya, Meiramova, Assel, Akhmetova, Zhanar, Kunz, Jeanette, Ainabekova, Bayan, Marotta, Francesco, Kushugulova, Almagul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253734/
https://www.ncbi.nlm.nih.gov/pubmed/37297889
http://dx.doi.org/10.3390/jcm12113694
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author Kozhakhmetov, Samat
Babenko, Dmitriy
Issilbayeva, Argul
Nurgaziyev, Madiyar
Kozhakhmetova, Saniya
Meiramova, Assel
Akhmetova, Zhanar
Kunz, Jeanette
Ainabekova, Bayan
Marotta, Francesco
Kushugulova, Almagul
author_facet Kozhakhmetov, Samat
Babenko, Dmitriy
Issilbayeva, Argul
Nurgaziyev, Madiyar
Kozhakhmetova, Saniya
Meiramova, Assel
Akhmetova, Zhanar
Kunz, Jeanette
Ainabekova, Bayan
Marotta, Francesco
Kushugulova, Almagul
author_sort Kozhakhmetov, Samat
collection PubMed
description This study aimed to identify the oral microbial signature of Kazakh female rheumatoid arthritis (RA) patients. A total of 75 female patients who met the American College of Rheumatology 2010 classification criteria for RA and 114 healthy volunteers were included in the study. Amplicons of the 16S rRNA gene were sequenced to analyze the microbial composition. We identified significant differences in bacterial diversity and abundance between the RA and control groups, as measured by Shannon (p value = 0.0205) and Simpson (p value = 0.00152) indices. The oral samples from RA patients had higher bacterial diversity than those from non-RA volunteers. The RA samples had a higher relative abundance of Prevotellaceae and Leptotrichiaceae, but a lower content of butyrate and propionate-producing bacteria compared to the control group. The samples from patients in remission had a higher abundance of Treponema sp. and Absconditabacteriales (SR1), whereas those with low disease activity had higher levels of Porphyromonas and those with high RA activity had higher levels of Staphylococcus. A positive correlation was found between the taxa Prevotella_9 and serum levels of antibodies to cyclic citrullinated peptide (ACPA) and rheumatoid factor (RF). The predicted functional pattern of the ACPA+/RF− and ACPA+/RF+ seropositive groups was characterized by increased ascorbate metabolism, degradation of glycosaminoglycans, and reduced biodegradation of xenobiotics. These findings suggest that the functional pattern of the microflora should be considered when selecting a therapeutic strategy for RA in order to provide a personalized approach.
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spelling pubmed-102537342023-06-10 Oral Microbial Signature of Rheumatoid Arthritis in Female Patients Kozhakhmetov, Samat Babenko, Dmitriy Issilbayeva, Argul Nurgaziyev, Madiyar Kozhakhmetova, Saniya Meiramova, Assel Akhmetova, Zhanar Kunz, Jeanette Ainabekova, Bayan Marotta, Francesco Kushugulova, Almagul J Clin Med Article This study aimed to identify the oral microbial signature of Kazakh female rheumatoid arthritis (RA) patients. A total of 75 female patients who met the American College of Rheumatology 2010 classification criteria for RA and 114 healthy volunteers were included in the study. Amplicons of the 16S rRNA gene were sequenced to analyze the microbial composition. We identified significant differences in bacterial diversity and abundance between the RA and control groups, as measured by Shannon (p value = 0.0205) and Simpson (p value = 0.00152) indices. The oral samples from RA patients had higher bacterial diversity than those from non-RA volunteers. The RA samples had a higher relative abundance of Prevotellaceae and Leptotrichiaceae, but a lower content of butyrate and propionate-producing bacteria compared to the control group. The samples from patients in remission had a higher abundance of Treponema sp. and Absconditabacteriales (SR1), whereas those with low disease activity had higher levels of Porphyromonas and those with high RA activity had higher levels of Staphylococcus. A positive correlation was found between the taxa Prevotella_9 and serum levels of antibodies to cyclic citrullinated peptide (ACPA) and rheumatoid factor (RF). The predicted functional pattern of the ACPA+/RF− and ACPA+/RF+ seropositive groups was characterized by increased ascorbate metabolism, degradation of glycosaminoglycans, and reduced biodegradation of xenobiotics. These findings suggest that the functional pattern of the microflora should be considered when selecting a therapeutic strategy for RA in order to provide a personalized approach. MDPI 2023-05-26 /pmc/articles/PMC10253734/ /pubmed/37297889 http://dx.doi.org/10.3390/jcm12113694 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kozhakhmetov, Samat
Babenko, Dmitriy
Issilbayeva, Argul
Nurgaziyev, Madiyar
Kozhakhmetova, Saniya
Meiramova, Assel
Akhmetova, Zhanar
Kunz, Jeanette
Ainabekova, Bayan
Marotta, Francesco
Kushugulova, Almagul
Oral Microbial Signature of Rheumatoid Arthritis in Female Patients
title Oral Microbial Signature of Rheumatoid Arthritis in Female Patients
title_full Oral Microbial Signature of Rheumatoid Arthritis in Female Patients
title_fullStr Oral Microbial Signature of Rheumatoid Arthritis in Female Patients
title_full_unstemmed Oral Microbial Signature of Rheumatoid Arthritis in Female Patients
title_short Oral Microbial Signature of Rheumatoid Arthritis in Female Patients
title_sort oral microbial signature of rheumatoid arthritis in female patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253734/
https://www.ncbi.nlm.nih.gov/pubmed/37297889
http://dx.doi.org/10.3390/jcm12113694
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