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Involvement of the Restoration of Cerebral Blood Flow and Maintenance of eNOS Expression in the Prophylactic Protective Effect of the Novel Ferulic Acid Derivative FAD012 against Ischemia/Reperfusion Injuries in Rats
Tissue plasminogen activator, aiming to restore cerebral blood flow (CBF), has been used for acute ischemic strokes in clinics; however, its narrow therapeutic time window remains a serious concern. To develop novel prophylactic drugs to alleviate cerebral ischemia/reperfusion injuries, ferulic acid...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253792/ https://www.ncbi.nlm.nih.gov/pubmed/37298615 http://dx.doi.org/10.3390/ijms24119663 |
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author | Asano, Takashi Xuan, Meiyan Iwata, Naohiro Takayama, Jun Hayashi, Kousuke Kato, Yosuke Aoyama, Toshiya Sugo, Hiroshi Matsuzaki, Hirokazu Yuan, Bo Kamiuchi, Shinya Hibino, Yasuhide Sakamoto, Takeshi Okazaki, Mari |
author_facet | Asano, Takashi Xuan, Meiyan Iwata, Naohiro Takayama, Jun Hayashi, Kousuke Kato, Yosuke Aoyama, Toshiya Sugo, Hiroshi Matsuzaki, Hirokazu Yuan, Bo Kamiuchi, Shinya Hibino, Yasuhide Sakamoto, Takeshi Okazaki, Mari |
author_sort | Asano, Takashi |
collection | PubMed |
description | Tissue plasminogen activator, aiming to restore cerebral blood flow (CBF), has been used for acute ischemic strokes in clinics; however, its narrow therapeutic time window remains a serious concern. To develop novel prophylactic drugs to alleviate cerebral ischemia/reperfusion injuries, ferulic acid derivative 012 (FAD012) was synthesized and showed comparable antioxidant properties to ferulic acid (FA) and probably possesses the potent ability to cross the blood–brain barrier. A more potent cytoprotective effect of FAD012 against H(2)O(2)-induced cytotoxicity in PC12 cells was also observed. In vivo toxicity was not observed in rats given a long-term oral administration of FAD012, indicating its good tolerability. A one-week-course oral administration of FAD012 significantly alleviated middle cerebral artery occlusion (MCAO)-induced cerebral ischemia/reperfusion injuries in rats, accompanied by the restoration of CBF and endothelial nitrogen oxide synthetase (eNOS) expression. Treatment with FAD012 significantly restored the cell viability and eNOS expression damaged by H(2)O(2), used to mimic MCAO-triggered oxidative stress, in rat brain microvascular endothelial cells. Our findings suggested that FAD012 protected the viability of vascular endothelium and maintained eNOS expression, ultimately contributing to the restoration of CBF, and may provide a rationale for the development of FAD012 into an effective prophylactic drug for patients at high risk of stroke. |
format | Online Article Text |
id | pubmed-10253792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102537922023-06-10 Involvement of the Restoration of Cerebral Blood Flow and Maintenance of eNOS Expression in the Prophylactic Protective Effect of the Novel Ferulic Acid Derivative FAD012 against Ischemia/Reperfusion Injuries in Rats Asano, Takashi Xuan, Meiyan Iwata, Naohiro Takayama, Jun Hayashi, Kousuke Kato, Yosuke Aoyama, Toshiya Sugo, Hiroshi Matsuzaki, Hirokazu Yuan, Bo Kamiuchi, Shinya Hibino, Yasuhide Sakamoto, Takeshi Okazaki, Mari Int J Mol Sci Article Tissue plasminogen activator, aiming to restore cerebral blood flow (CBF), has been used for acute ischemic strokes in clinics; however, its narrow therapeutic time window remains a serious concern. To develop novel prophylactic drugs to alleviate cerebral ischemia/reperfusion injuries, ferulic acid derivative 012 (FAD012) was synthesized and showed comparable antioxidant properties to ferulic acid (FA) and probably possesses the potent ability to cross the blood–brain barrier. A more potent cytoprotective effect of FAD012 against H(2)O(2)-induced cytotoxicity in PC12 cells was also observed. In vivo toxicity was not observed in rats given a long-term oral administration of FAD012, indicating its good tolerability. A one-week-course oral administration of FAD012 significantly alleviated middle cerebral artery occlusion (MCAO)-induced cerebral ischemia/reperfusion injuries in rats, accompanied by the restoration of CBF and endothelial nitrogen oxide synthetase (eNOS) expression. Treatment with FAD012 significantly restored the cell viability and eNOS expression damaged by H(2)O(2), used to mimic MCAO-triggered oxidative stress, in rat brain microvascular endothelial cells. Our findings suggested that FAD012 protected the viability of vascular endothelium and maintained eNOS expression, ultimately contributing to the restoration of CBF, and may provide a rationale for the development of FAD012 into an effective prophylactic drug for patients at high risk of stroke. MDPI 2023-06-02 /pmc/articles/PMC10253792/ /pubmed/37298615 http://dx.doi.org/10.3390/ijms24119663 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Asano, Takashi Xuan, Meiyan Iwata, Naohiro Takayama, Jun Hayashi, Kousuke Kato, Yosuke Aoyama, Toshiya Sugo, Hiroshi Matsuzaki, Hirokazu Yuan, Bo Kamiuchi, Shinya Hibino, Yasuhide Sakamoto, Takeshi Okazaki, Mari Involvement of the Restoration of Cerebral Blood Flow and Maintenance of eNOS Expression in the Prophylactic Protective Effect of the Novel Ferulic Acid Derivative FAD012 against Ischemia/Reperfusion Injuries in Rats |
title | Involvement of the Restoration of Cerebral Blood Flow and Maintenance of eNOS Expression in the Prophylactic Protective Effect of the Novel Ferulic Acid Derivative FAD012 against Ischemia/Reperfusion Injuries in Rats |
title_full | Involvement of the Restoration of Cerebral Blood Flow and Maintenance of eNOS Expression in the Prophylactic Protective Effect of the Novel Ferulic Acid Derivative FAD012 against Ischemia/Reperfusion Injuries in Rats |
title_fullStr | Involvement of the Restoration of Cerebral Blood Flow and Maintenance of eNOS Expression in the Prophylactic Protective Effect of the Novel Ferulic Acid Derivative FAD012 against Ischemia/Reperfusion Injuries in Rats |
title_full_unstemmed | Involvement of the Restoration of Cerebral Blood Flow and Maintenance of eNOS Expression in the Prophylactic Protective Effect of the Novel Ferulic Acid Derivative FAD012 against Ischemia/Reperfusion Injuries in Rats |
title_short | Involvement of the Restoration of Cerebral Blood Flow and Maintenance of eNOS Expression in the Prophylactic Protective Effect of the Novel Ferulic Acid Derivative FAD012 against Ischemia/Reperfusion Injuries in Rats |
title_sort | involvement of the restoration of cerebral blood flow and maintenance of enos expression in the prophylactic protective effect of the novel ferulic acid derivative fad012 against ischemia/reperfusion injuries in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253792/ https://www.ncbi.nlm.nih.gov/pubmed/37298615 http://dx.doi.org/10.3390/ijms24119663 |
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