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Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice

Achillea fragrantissima, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant Staphylococcus aureus (MRSA) and multi-drug-res...

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Autores principales: Almuhanna, Yasir, Alqasmi, Mohammed Hussein, AlSudais, Hamood, Alrouji, Mohammed, Kuriri, Fahd A., Alissa, Mohammed, Alsuwat, Meshari A., Asad, Mohammed, Joseph, Babu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253793/
https://www.ncbi.nlm.nih.gov/pubmed/37298725
http://dx.doi.org/10.3390/ijms24119774
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author Almuhanna, Yasir
Alqasmi, Mohammed Hussein
AlSudais, Hamood
Alrouji, Mohammed
Kuriri, Fahd A.
Alissa, Mohammed
Alsuwat, Meshari A.
Asad, Mohammed
Joseph, Babu
author_facet Almuhanna, Yasir
Alqasmi, Mohammed Hussein
AlSudais, Hamood
Alrouji, Mohammed
Kuriri, Fahd A.
Alissa, Mohammed
Alsuwat, Meshari A.
Asad, Mohammed
Joseph, Babu
author_sort Almuhanna, Yasir
collection PubMed
description Achillea fragrantissima, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant Staphylococcus aureus (MRSA) and multi-drug-resistant Pseudomonas aeruginosa (MDR-P. aeruginosa) using in vitro and in vivo studies. A biofilm model induced through an excision wound in diabetic mice was used to evaluate its effect in vivo. The skin irritation and cytotoxic effects of the extract were determined using mice and HaCaT cell lines, respectively. The Achillea fragrantissima methanolic extract was analyzed with LC-MS to detect different phytoconstituents, which revealed the presence of 47 different phytoconstituents. The extract inhibited the growth of both tested pathogens in vitro. It also increased the healing of biofilm-formed excision wounds, demonstrating its antibiofilm, antimicrobial, and wound-healing action in vivo. The effect of the extract was concentration-dependent, and its activity was stronger against MRSA than MDR-P. aeruginosa. The extract formulation was devoid of a skin irritation effect in vivo and cytotoxic effect on HaCaT cell lines in vitro.
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spelling pubmed-102537932023-06-10 Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice Almuhanna, Yasir Alqasmi, Mohammed Hussein AlSudais, Hamood Alrouji, Mohammed Kuriri, Fahd A. Alissa, Mohammed Alsuwat, Meshari A. Asad, Mohammed Joseph, Babu Int J Mol Sci Article Achillea fragrantissima, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant Staphylococcus aureus (MRSA) and multi-drug-resistant Pseudomonas aeruginosa (MDR-P. aeruginosa) using in vitro and in vivo studies. A biofilm model induced through an excision wound in diabetic mice was used to evaluate its effect in vivo. The skin irritation and cytotoxic effects of the extract were determined using mice and HaCaT cell lines, respectively. The Achillea fragrantissima methanolic extract was analyzed with LC-MS to detect different phytoconstituents, which revealed the presence of 47 different phytoconstituents. The extract inhibited the growth of both tested pathogens in vitro. It also increased the healing of biofilm-formed excision wounds, demonstrating its antibiofilm, antimicrobial, and wound-healing action in vivo. The effect of the extract was concentration-dependent, and its activity was stronger against MRSA than MDR-P. aeruginosa. The extract formulation was devoid of a skin irritation effect in vivo and cytotoxic effect on HaCaT cell lines in vitro. MDPI 2023-06-05 /pmc/articles/PMC10253793/ /pubmed/37298725 http://dx.doi.org/10.3390/ijms24119774 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Almuhanna, Yasir
Alqasmi, Mohammed Hussein
AlSudais, Hamood
Alrouji, Mohammed
Kuriri, Fahd A.
Alissa, Mohammed
Alsuwat, Meshari A.
Asad, Mohammed
Joseph, Babu
Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice
title Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice
title_full Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice
title_fullStr Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice
title_full_unstemmed Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice
title_short Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice
title_sort effect of achillea fragrantissima extract on excision wound biofilms of mrsa and pseudomonas aeruginosa in diabetic mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253793/
https://www.ncbi.nlm.nih.gov/pubmed/37298725
http://dx.doi.org/10.3390/ijms24119774
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