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Increased Alveolar Epithelial Damage Markers and Inflammasome-Regulated Cytokines Are Associated with Pulmonary Superinfection in ARDS

Acute respiratory distress syndrome (ARDS) is a life-threatening form of respiratory failure defined by dysregulated immune homeostasis and alveolar epithelial and endothelial damage. Up to 40% of ARDS patients develop pulmonary superinfections, contributing to poor prognosis and increasing mortalit...

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Autores principales: Peukert, Konrad, Sauer, Andrea, Seeliger, Benjamin, Feuerborn, Caroline, Fox, Mario, Schulz, Susanne, Wild, Lennart, Borger, Valeri, Schuss, Patrick, Schneider, Matthias, Güresir, Erdem, Coburn, Mark, Putensen, Christian, Wilhelm, Christoph, Bode, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253810/
https://www.ncbi.nlm.nih.gov/pubmed/37297845
http://dx.doi.org/10.3390/jcm12113649
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author Peukert, Konrad
Sauer, Andrea
Seeliger, Benjamin
Feuerborn, Caroline
Fox, Mario
Schulz, Susanne
Wild, Lennart
Borger, Valeri
Schuss, Patrick
Schneider, Matthias
Güresir, Erdem
Coburn, Mark
Putensen, Christian
Wilhelm, Christoph
Bode, Christian
author_facet Peukert, Konrad
Sauer, Andrea
Seeliger, Benjamin
Feuerborn, Caroline
Fox, Mario
Schulz, Susanne
Wild, Lennart
Borger, Valeri
Schuss, Patrick
Schneider, Matthias
Güresir, Erdem
Coburn, Mark
Putensen, Christian
Wilhelm, Christoph
Bode, Christian
author_sort Peukert, Konrad
collection PubMed
description Acute respiratory distress syndrome (ARDS) is a life-threatening form of respiratory failure defined by dysregulated immune homeostasis and alveolar epithelial and endothelial damage. Up to 40% of ARDS patients develop pulmonary superinfections, contributing to poor prognosis and increasing mortality. Understanding what renders ARDS patients highly susceptible to pulmonary superinfections is therefore essential. We hypothesized that ARDS patients who develop pulmonary superinfections display a distinct pulmonary injury and pro-inflammatory response pattern. Serum and BALF samples from 52 patients were collected simultaneously within 24 h of ARDS onset. The incidence of pulmonary superinfections was determined retrospectively, and the patients were classified accordingly. Serum concentrations of the epithelial markers soluble receptor for advanced glycation end-products (sRAGE) and surfactant protein D (SP-D) and the endothelial markers vascular endothelial growth factor (VEGF) and angiopoetin-2 (Ang-2) as well as bronchoalveolar lavage fluid concentrations of the pro-inflammatory cytokines interleukin 1ß (IL-1ß), interleukin 18 (IL-18), interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-a) were analyzed via multiplex immunoassay. Inflammasome-regulated cytokine IL-18 and the epithelial damage markers SP-D and sRAGE were significantly increased in ARDS patients who developed pulmonary superinfections. In contrast, endothelial markers and inflammasome-independent cytokines did not differ between the groups. The current findings reveal a distinct biomarker pattern that indicates inflammasome activation and alveolar epithelial injury. This pattern may potentially be used in future studies to identify high-risk patients, enabling targeted preventive strategies and personalized treatment approaches.
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spelling pubmed-102538102023-06-10 Increased Alveolar Epithelial Damage Markers and Inflammasome-Regulated Cytokines Are Associated with Pulmonary Superinfection in ARDS Peukert, Konrad Sauer, Andrea Seeliger, Benjamin Feuerborn, Caroline Fox, Mario Schulz, Susanne Wild, Lennart Borger, Valeri Schuss, Patrick Schneider, Matthias Güresir, Erdem Coburn, Mark Putensen, Christian Wilhelm, Christoph Bode, Christian J Clin Med Article Acute respiratory distress syndrome (ARDS) is a life-threatening form of respiratory failure defined by dysregulated immune homeostasis and alveolar epithelial and endothelial damage. Up to 40% of ARDS patients develop pulmonary superinfections, contributing to poor prognosis and increasing mortality. Understanding what renders ARDS patients highly susceptible to pulmonary superinfections is therefore essential. We hypothesized that ARDS patients who develop pulmonary superinfections display a distinct pulmonary injury and pro-inflammatory response pattern. Serum and BALF samples from 52 patients were collected simultaneously within 24 h of ARDS onset. The incidence of pulmonary superinfections was determined retrospectively, and the patients were classified accordingly. Serum concentrations of the epithelial markers soluble receptor for advanced glycation end-products (sRAGE) and surfactant protein D (SP-D) and the endothelial markers vascular endothelial growth factor (VEGF) and angiopoetin-2 (Ang-2) as well as bronchoalveolar lavage fluid concentrations of the pro-inflammatory cytokines interleukin 1ß (IL-1ß), interleukin 18 (IL-18), interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-a) were analyzed via multiplex immunoassay. Inflammasome-regulated cytokine IL-18 and the epithelial damage markers SP-D and sRAGE were significantly increased in ARDS patients who developed pulmonary superinfections. In contrast, endothelial markers and inflammasome-independent cytokines did not differ between the groups. The current findings reveal a distinct biomarker pattern that indicates inflammasome activation and alveolar epithelial injury. This pattern may potentially be used in future studies to identify high-risk patients, enabling targeted preventive strategies and personalized treatment approaches. MDPI 2023-05-24 /pmc/articles/PMC10253810/ /pubmed/37297845 http://dx.doi.org/10.3390/jcm12113649 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peukert, Konrad
Sauer, Andrea
Seeliger, Benjamin
Feuerborn, Caroline
Fox, Mario
Schulz, Susanne
Wild, Lennart
Borger, Valeri
Schuss, Patrick
Schneider, Matthias
Güresir, Erdem
Coburn, Mark
Putensen, Christian
Wilhelm, Christoph
Bode, Christian
Increased Alveolar Epithelial Damage Markers and Inflammasome-Regulated Cytokines Are Associated with Pulmonary Superinfection in ARDS
title Increased Alveolar Epithelial Damage Markers and Inflammasome-Regulated Cytokines Are Associated with Pulmonary Superinfection in ARDS
title_full Increased Alveolar Epithelial Damage Markers and Inflammasome-Regulated Cytokines Are Associated with Pulmonary Superinfection in ARDS
title_fullStr Increased Alveolar Epithelial Damage Markers and Inflammasome-Regulated Cytokines Are Associated with Pulmonary Superinfection in ARDS
title_full_unstemmed Increased Alveolar Epithelial Damage Markers and Inflammasome-Regulated Cytokines Are Associated with Pulmonary Superinfection in ARDS
title_short Increased Alveolar Epithelial Damage Markers and Inflammasome-Regulated Cytokines Are Associated with Pulmonary Superinfection in ARDS
title_sort increased alveolar epithelial damage markers and inflammasome-regulated cytokines are associated with pulmonary superinfection in ards
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253810/
https://www.ncbi.nlm.nih.gov/pubmed/37297845
http://dx.doi.org/10.3390/jcm12113649
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