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MsSPL9 Modulates Nodulation under Nitrate Sufficiency Condition in Medicago sativa

Nodulation in Leguminous spp. is induced by common environmental cues, such as low nitrogen availability conditions, in the presence of the specific Rhizobium spp. in the rhizosphere. Medicago sativa (alfalfa) is an important nitrogen-fixing forage crop that is widely cultivated around the world and...

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Autores principales: Nasrollahi, Vida, Allam, Gamalat, Kohalmi, Susanne E., Hannoufa, Abdelali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253965/
https://www.ncbi.nlm.nih.gov/pubmed/37298564
http://dx.doi.org/10.3390/ijms24119615
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author Nasrollahi, Vida
Allam, Gamalat
Kohalmi, Susanne E.
Hannoufa, Abdelali
author_facet Nasrollahi, Vida
Allam, Gamalat
Kohalmi, Susanne E.
Hannoufa, Abdelali
author_sort Nasrollahi, Vida
collection PubMed
description Nodulation in Leguminous spp. is induced by common environmental cues, such as low nitrogen availability conditions, in the presence of the specific Rhizobium spp. in the rhizosphere. Medicago sativa (alfalfa) is an important nitrogen-fixing forage crop that is widely cultivated around the world and relied upon as a staple source of forage in livestock feed. Although alfalfa’s relationship with these bacteria is one of the most efficient between rhizobia and legume plants, breeding for nitrogen-related traits in this crop has received little attention. In this report, we investigate the role of Squamosa-Promoter Binding Protein-Like 9 (SPL9), a target of miR156, in nodulation in alfalfa. Transgenic alfalfa plants with SPL9-silenced (SPL9-RNAi) and overexpressed (35S::SPL9) were compared to wild-type (WT) alfalfa for phenotypic changes in nodulation in the presence and absence of nitrogen. Phenotypic analyses showed that silencing of MsSPL9 in alfalfa caused an increase in the number of nodules. Moreover, the characterization of phenotypic and molecular parameters revealed that MsSPL9 regulates nodulation under a high concentration of nitrate (10 mM KNO(3)) by regulating the transcription levels of the nitrate-responsive genes Nitrate Reductase1 (NR1), NR2, Nitrate transporter 2.5 (NRT2.5), and a shoot-controlled autoregulation of nodulation (AON) gene, Super numeric nodules (SUNN). While MsSPL9–overexpressing transgenic plants have dramatically increased transcript levels of SUNN, NR1, NR2, and NRT2.5, reducing MsSPL9 caused downregulation of these genes and displayed a nitrogen-starved phenotype, as downregulation of the MsSPL9 transcript levels caused a nitrate-tolerant nodulation phenotype. Taken together, our results suggest that MsSPL9 regulates nodulation in alfalfa in response to nitrate.
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spelling pubmed-102539652023-06-10 MsSPL9 Modulates Nodulation under Nitrate Sufficiency Condition in Medicago sativa Nasrollahi, Vida Allam, Gamalat Kohalmi, Susanne E. Hannoufa, Abdelali Int J Mol Sci Article Nodulation in Leguminous spp. is induced by common environmental cues, such as low nitrogen availability conditions, in the presence of the specific Rhizobium spp. in the rhizosphere. Medicago sativa (alfalfa) is an important nitrogen-fixing forage crop that is widely cultivated around the world and relied upon as a staple source of forage in livestock feed. Although alfalfa’s relationship with these bacteria is one of the most efficient between rhizobia and legume plants, breeding for nitrogen-related traits in this crop has received little attention. In this report, we investigate the role of Squamosa-Promoter Binding Protein-Like 9 (SPL9), a target of miR156, in nodulation in alfalfa. Transgenic alfalfa plants with SPL9-silenced (SPL9-RNAi) and overexpressed (35S::SPL9) were compared to wild-type (WT) alfalfa for phenotypic changes in nodulation in the presence and absence of nitrogen. Phenotypic analyses showed that silencing of MsSPL9 in alfalfa caused an increase in the number of nodules. Moreover, the characterization of phenotypic and molecular parameters revealed that MsSPL9 regulates nodulation under a high concentration of nitrate (10 mM KNO(3)) by regulating the transcription levels of the nitrate-responsive genes Nitrate Reductase1 (NR1), NR2, Nitrate transporter 2.5 (NRT2.5), and a shoot-controlled autoregulation of nodulation (AON) gene, Super numeric nodules (SUNN). While MsSPL9–overexpressing transgenic plants have dramatically increased transcript levels of SUNN, NR1, NR2, and NRT2.5, reducing MsSPL9 caused downregulation of these genes and displayed a nitrogen-starved phenotype, as downregulation of the MsSPL9 transcript levels caused a nitrate-tolerant nodulation phenotype. Taken together, our results suggest that MsSPL9 regulates nodulation in alfalfa in response to nitrate. MDPI 2023-06-01 /pmc/articles/PMC10253965/ /pubmed/37298564 http://dx.doi.org/10.3390/ijms24119615 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nasrollahi, Vida
Allam, Gamalat
Kohalmi, Susanne E.
Hannoufa, Abdelali
MsSPL9 Modulates Nodulation under Nitrate Sufficiency Condition in Medicago sativa
title MsSPL9 Modulates Nodulation under Nitrate Sufficiency Condition in Medicago sativa
title_full MsSPL9 Modulates Nodulation under Nitrate Sufficiency Condition in Medicago sativa
title_fullStr MsSPL9 Modulates Nodulation under Nitrate Sufficiency Condition in Medicago sativa
title_full_unstemmed MsSPL9 Modulates Nodulation under Nitrate Sufficiency Condition in Medicago sativa
title_short MsSPL9 Modulates Nodulation under Nitrate Sufficiency Condition in Medicago sativa
title_sort msspl9 modulates nodulation under nitrate sufficiency condition in medicago sativa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10253965/
https://www.ncbi.nlm.nih.gov/pubmed/37298564
http://dx.doi.org/10.3390/ijms24119615
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