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Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms

The aim of this study was to determine the bactericidal potential of graphene oxide (GO) in contact with four species of bacteria: E. coli, S. mutans, S. aureus and E. faecalis. Bacterial cell suspensions of each species were incubated in a medium containing GO, with incubation times of 5, 10, 30 an...

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Autores principales: Olczak, Katarzyna, Jakubowski, Witold, Szymański, Witold
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10254371/
https://www.ncbi.nlm.nih.gov/pubmed/37297332
http://dx.doi.org/10.3390/ma16114199
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author Olczak, Katarzyna
Jakubowski, Witold
Szymański, Witold
author_facet Olczak, Katarzyna
Jakubowski, Witold
Szymański, Witold
author_sort Olczak, Katarzyna
collection PubMed
description The aim of this study was to determine the bactericidal potential of graphene oxide (GO) in contact with four species of bacteria: E. coli, S. mutans, S. aureus and E. faecalis. Bacterial cell suspensions of each species were incubated in a medium containing GO, with incubation times of 5, 10, 30 and 60 min, at final concentrations of 50, 100, 200, 300 and 500 μg/mL. The cytotoxicity of GO was evaluated using live/dead staining. The results were recorded using a BD Accuri C6 flow cytofluorimeter. Obtained data were analyzed using BD CSampler software. A significant bacteria viability reduction was noted in all GO-containing samples. The antibacterial properties of GO were strongly influenced by GO concentration and incubation time. The highest bactericidal activity was observed at concentrations of 300 and 500 μg/mL for all incubation times (5, 10, 30 and 60 min). The highest antimicrobial potential was observed for E. coli: after 60 min, the mortality rate was 94% at 300 µg/mL GO and 96% at 500 µg/mL GO; the lowest was found for S. aureus—49% (300 µg/mL) and 55% (500 µg/mL).
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spelling pubmed-102543712023-06-10 Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms Olczak, Katarzyna Jakubowski, Witold Szymański, Witold Materials (Basel) Article The aim of this study was to determine the bactericidal potential of graphene oxide (GO) in contact with four species of bacteria: E. coli, S. mutans, S. aureus and E. faecalis. Bacterial cell suspensions of each species were incubated in a medium containing GO, with incubation times of 5, 10, 30 and 60 min, at final concentrations of 50, 100, 200, 300 and 500 μg/mL. The cytotoxicity of GO was evaluated using live/dead staining. The results were recorded using a BD Accuri C6 flow cytofluorimeter. Obtained data were analyzed using BD CSampler software. A significant bacteria viability reduction was noted in all GO-containing samples. The antibacterial properties of GO were strongly influenced by GO concentration and incubation time. The highest bactericidal activity was observed at concentrations of 300 and 500 μg/mL for all incubation times (5, 10, 30 and 60 min). The highest antimicrobial potential was observed for E. coli: after 60 min, the mortality rate was 94% at 300 µg/mL GO and 96% at 500 µg/mL GO; the lowest was found for S. aureus—49% (300 µg/mL) and 55% (500 µg/mL). MDPI 2023-06-05 /pmc/articles/PMC10254371/ /pubmed/37297332 http://dx.doi.org/10.3390/ma16114199 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olczak, Katarzyna
Jakubowski, Witold
Szymański, Witold
Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms
title Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms
title_full Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms
title_fullStr Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms
title_full_unstemmed Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms
title_short Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms
title_sort bactericidal activity of graphene oxide tests for selected microorganisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10254371/
https://www.ncbi.nlm.nih.gov/pubmed/37297332
http://dx.doi.org/10.3390/ma16114199
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