Cargando…

Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings

Herein, polydopamine (PDA)-based antimicrobial coatings loaded with silver nanoparticles (Ag NPs) and gentamicin were designed and prepared on glass slides using two different approaches. To our knowledge, this study was performed for the first time with the aim to compare these methods (viz., in si...

Descripción completa

Detalles Bibliográficos
Autores principales: Batul, Rahila, Bhave, Mrinal, Yu, Aimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10254676/
https://www.ncbi.nlm.nih.gov/pubmed/37298735
http://dx.doi.org/10.3390/molecules28114258
_version_ 1785056699773616128
author Batul, Rahila
Bhave, Mrinal
Yu, Aimin
author_facet Batul, Rahila
Bhave, Mrinal
Yu, Aimin
author_sort Batul, Rahila
collection PubMed
description Herein, polydopamine (PDA)-based antimicrobial coatings loaded with silver nanoparticles (Ag NPs) and gentamicin were designed and prepared on glass slides using two different approaches. To our knowledge, this study was performed for the first time with the aim to compare these methods (viz., in situ loading and physical adsorption method) regarding the loading and release behavior of payloads. In one method, gentamicin was in situ loaded on PDA-coated substrates during PDA polymerization followed by Ag NPs immobilization (named as Ag@Gen/PDA); for the second method, Ag NPs and gentamicin were simultaneously loaded onto PDA via physical adsorption by immersing pre-formed PDA coatings into a mixed solution of Ag NPs and gentamicin (named as Ag/Gen@PDA). The loading and release characteristics of these antimicrobial coatings were compared, and both gave variable outcomes. The in situ loading method consequently provided a relatively slow release of loaded antimicrobials, i.e., approx. 46% for Ag@Gen/PDA as compared to 92% from physically adsorbed Ag/GenPDA in an immersion period of 30 days. A similar trend was observed for gentamicin release, i.e., ~0.006 µg/mL from Ag@Gen/PDA and 0.02 µg/mL from Ag/Gen@PDA each day. The slower antimicrobial release from Ag@Gen/PDA coatings would ultimately provide an effective long-term antimicrobial property as compared to Ag/Gen@PDA. Finally, the synergistic antimicrobial activities of these composite coatings were assessed against two microbial species, namely, Staphylococcus aureus and Escherichia coli, hence providing evidence in the prevention of bacterial colonization.
format Online
Article
Text
id pubmed-10254676
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102546762023-06-10 Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings Batul, Rahila Bhave, Mrinal Yu, Aimin Molecules Article Herein, polydopamine (PDA)-based antimicrobial coatings loaded with silver nanoparticles (Ag NPs) and gentamicin were designed and prepared on glass slides using two different approaches. To our knowledge, this study was performed for the first time with the aim to compare these methods (viz., in situ loading and physical adsorption method) regarding the loading and release behavior of payloads. In one method, gentamicin was in situ loaded on PDA-coated substrates during PDA polymerization followed by Ag NPs immobilization (named as Ag@Gen/PDA); for the second method, Ag NPs and gentamicin were simultaneously loaded onto PDA via physical adsorption by immersing pre-formed PDA coatings into a mixed solution of Ag NPs and gentamicin (named as Ag/Gen@PDA). The loading and release characteristics of these antimicrobial coatings were compared, and both gave variable outcomes. The in situ loading method consequently provided a relatively slow release of loaded antimicrobials, i.e., approx. 46% for Ag@Gen/PDA as compared to 92% from physically adsorbed Ag/GenPDA in an immersion period of 30 days. A similar trend was observed for gentamicin release, i.e., ~0.006 µg/mL from Ag@Gen/PDA and 0.02 µg/mL from Ag/Gen@PDA each day. The slower antimicrobial release from Ag@Gen/PDA coatings would ultimately provide an effective long-term antimicrobial property as compared to Ag/Gen@PDA. Finally, the synergistic antimicrobial activities of these composite coatings were assessed against two microbial species, namely, Staphylococcus aureus and Escherichia coli, hence providing evidence in the prevention of bacterial colonization. MDPI 2023-05-23 /pmc/articles/PMC10254676/ /pubmed/37298735 http://dx.doi.org/10.3390/molecules28114258 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Batul, Rahila
Bhave, Mrinal
Yu, Aimin
Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings
title Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings
title_full Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings
title_fullStr Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings
title_full_unstemmed Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings
title_short Investigation of Antimicrobial Effects of Polydopamine-Based Composite Coatings
title_sort investigation of antimicrobial effects of polydopamine-based composite coatings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10254676/
https://www.ncbi.nlm.nih.gov/pubmed/37298735
http://dx.doi.org/10.3390/molecules28114258
work_keys_str_mv AT batulrahila investigationofantimicrobialeffectsofpolydopaminebasedcompositecoatings
AT bhavemrinal investigationofantimicrobialeffectsofpolydopaminebasedcompositecoatings
AT yuaimin investigationofantimicrobialeffectsofpolydopaminebasedcompositecoatings