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Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study

Background: Postprandial metabolomic profiles and their inter-individual variability are not well characterised. Here, we describe postprandial metabolite changes, their correlations with fasting values and their inter- and intra-individual variability, following a standardised meal in the ZOE PREDI...

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Autores principales: Bermingham, Kate M., Mazidi, Mohsen, Franks, Paul W., Maher, Tyler, Valdes, Ana M., Linenberg, Inbar, Wolf, Jonathan, Hadjigeorgiou, George, Spector, Tim D., Menni, Cristina, Ordovas, Jose M., Berry, Sarah E., Hall, Wendy L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255657/
https://www.ncbi.nlm.nih.gov/pubmed/37299601
http://dx.doi.org/10.3390/nu15112638
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author Bermingham, Kate M.
Mazidi, Mohsen
Franks, Paul W.
Maher, Tyler
Valdes, Ana M.
Linenberg, Inbar
Wolf, Jonathan
Hadjigeorgiou, George
Spector, Tim D.
Menni, Cristina
Ordovas, Jose M.
Berry, Sarah E.
Hall, Wendy L.
author_facet Bermingham, Kate M.
Mazidi, Mohsen
Franks, Paul W.
Maher, Tyler
Valdes, Ana M.
Linenberg, Inbar
Wolf, Jonathan
Hadjigeorgiou, George
Spector, Tim D.
Menni, Cristina
Ordovas, Jose M.
Berry, Sarah E.
Hall, Wendy L.
author_sort Bermingham, Kate M.
collection PubMed
description Background: Postprandial metabolomic profiles and their inter-individual variability are not well characterised. Here, we describe postprandial metabolite changes, their correlations with fasting values and their inter- and intra-individual variability, following a standardised meal in the ZOE PREDICT 1 cohort. Methods: In the ZOE PREDICT 1 study (n = 1002 (NCT03479866)), 250 metabolites, mainly lipids, were measured by a Nightingale NMR panel in fasting and postprandial (4 and 6 h after a 3.7 MJ mixed nutrient meal, with a second 2.2 MJ mixed nutrient meal at 4 h) serum samples. For each metabolite, inter- and intra-individual variability over time was evaluated using linear mixed modelling and intraclass correlation coefficients (ICC) were calculated. Results: Postprandially, 85% (of 250 metabolites) significantly changed from fasting at 6 h (47% increased, 53% decreased; Kruskal–Wallis), with 37 measures increasing by >25% and 14 increasing by >50%. The largest changes were observed in very large lipoprotein particles and ketone bodies. Seventy-one percent of circulating metabolites were strongly correlated (Spearman’s rho >0.80) between fasting and postprandial timepoints, and 5% were weakly correlated (rho <0.50). The median ICC of the 250 metabolites was 0.91 (range 0.08–0.99). The lowest ICCs (ICC <0.40, 4% of measures) were found for glucose, pyruvate, ketone bodies (β-hydroxybutyrate, acetoacetate, acetate) and lactate. Conclusions: In this large-scale postprandial metabolomic study, circulating metabolites were highly variable between individuals following sequential mixed meals. Findings suggest that a meal challenge may yield postprandial responses divergent from fasting measures, specifically for glycolysis, essential amino acid, ketone body and lipoprotein size metabolites.
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spelling pubmed-102556572023-06-10 Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study Bermingham, Kate M. Mazidi, Mohsen Franks, Paul W. Maher, Tyler Valdes, Ana M. Linenberg, Inbar Wolf, Jonathan Hadjigeorgiou, George Spector, Tim D. Menni, Cristina Ordovas, Jose M. Berry, Sarah E. Hall, Wendy L. Nutrients Article Background: Postprandial metabolomic profiles and their inter-individual variability are not well characterised. Here, we describe postprandial metabolite changes, their correlations with fasting values and their inter- and intra-individual variability, following a standardised meal in the ZOE PREDICT 1 cohort. Methods: In the ZOE PREDICT 1 study (n = 1002 (NCT03479866)), 250 metabolites, mainly lipids, were measured by a Nightingale NMR panel in fasting and postprandial (4 and 6 h after a 3.7 MJ mixed nutrient meal, with a second 2.2 MJ mixed nutrient meal at 4 h) serum samples. For each metabolite, inter- and intra-individual variability over time was evaluated using linear mixed modelling and intraclass correlation coefficients (ICC) were calculated. Results: Postprandially, 85% (of 250 metabolites) significantly changed from fasting at 6 h (47% increased, 53% decreased; Kruskal–Wallis), with 37 measures increasing by >25% and 14 increasing by >50%. The largest changes were observed in very large lipoprotein particles and ketone bodies. Seventy-one percent of circulating metabolites were strongly correlated (Spearman’s rho >0.80) between fasting and postprandial timepoints, and 5% were weakly correlated (rho <0.50). The median ICC of the 250 metabolites was 0.91 (range 0.08–0.99). The lowest ICCs (ICC <0.40, 4% of measures) were found for glucose, pyruvate, ketone bodies (β-hydroxybutyrate, acetoacetate, acetate) and lactate. Conclusions: In this large-scale postprandial metabolomic study, circulating metabolites were highly variable between individuals following sequential mixed meals. Findings suggest that a meal challenge may yield postprandial responses divergent from fasting measures, specifically for glycolysis, essential amino acid, ketone body and lipoprotein size metabolites. MDPI 2023-06-05 /pmc/articles/PMC10255657/ /pubmed/37299601 http://dx.doi.org/10.3390/nu15112638 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bermingham, Kate M.
Mazidi, Mohsen
Franks, Paul W.
Maher, Tyler
Valdes, Ana M.
Linenberg, Inbar
Wolf, Jonathan
Hadjigeorgiou, George
Spector, Tim D.
Menni, Cristina
Ordovas, Jose M.
Berry, Sarah E.
Hall, Wendy L.
Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study
title Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study
title_full Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study
title_fullStr Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study
title_full_unstemmed Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study
title_short Characterisation of Fasting and Postprandial NMR Metabolites: Insights from the ZOE PREDICT 1 Study
title_sort characterisation of fasting and postprandial nmr metabolites: insights from the zoe predict 1 study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255657/
https://www.ncbi.nlm.nih.gov/pubmed/37299601
http://dx.doi.org/10.3390/nu15112638
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