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Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities

Background: Artemisia sieberi (mugwort) is a member of the daisy family Asteraceae and is widely propagated in Saudi Arabia. A. sieberi has historical medical importance in traditional societies. The current study aimed to assess the antibacterial and antifungal characteristics of the aqueous and et...

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Autores principales: Al-Otibi, Fatimah, Alshammry, Nourah A., Alharbi, Raedah I., Bin-Jumah, May N., AlSubaie, Maha M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255823/
https://www.ncbi.nlm.nih.gov/pubmed/37299074
http://dx.doi.org/10.3390/plants12112093
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author Al-Otibi, Fatimah
Alshammry, Nourah A.
Alharbi, Raedah I.
Bin-Jumah, May N.
AlSubaie, Maha M.
author_facet Al-Otibi, Fatimah
Alshammry, Nourah A.
Alharbi, Raedah I.
Bin-Jumah, May N.
AlSubaie, Maha M.
author_sort Al-Otibi, Fatimah
collection PubMed
description Background: Artemisia sieberi (mugwort) is a member of the daisy family Asteraceae and is widely propagated in Saudi Arabia. A. sieberi has historical medical importance in traditional societies. The current study aimed to assess the antibacterial and antifungal characteristics of the aqueous and ethanolic extracts of A. sieberi. In addition, the study investigated the effect of silver nanoparticles (AgNPs) synthesized from the A. sieberi extract. Methods: The ethanolic and aqueous extracts and AgNPs were prepared from the shoots of A. sieberi. The characteristics of AgNPs were assessed by UV–visible spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS). The antibacterial experiments were performed against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. The fungal species used were Candida parapsilosis, Candida krusei, Candida famata, Candida rhodotorula, and Candida albicans. The antibacterial and antifungal characteristics were evaluated by measuring the diameter of growing organisms in Petri dishes treated with different concentrations of either extracts or AgNPs compared to the untreated controls. Furthermore, TEM imaging was used to investigate any ultrastructure changes in the microbes treated with crude extracts and AgNO(3). Results: The ethanolic and aqueous extracts significantly decreased the growth of E. coli, S. aureus, and B. subtilis (p < 0.001), while P. aeruginosa was not affected. Unlike crude extracts, AgNPs had more substantial antibacterial effects against all species. In addition, the mycelial growth of C. famata was reduced by the treatment of both extracts. C. krusei mycelial growth was decreased by the aqueous extract, while the growth of C. parapsilosis was affected by the ethanolic extract and AgNPs (p < 0.001). None of the treatments affected the growth of C. albicans or C. rhodotorula. TEM analysis showed cellular ultrastructure changes in the treated S. aureus and C. famata compared to the control. Conclusion: The biosynthesized AgNPs and extracts of A. sieberi have a potential antimicrobial characteristic against pathogenic bacterial and fungal strains and nullified resistance behavior.
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spelling pubmed-102558232023-06-10 Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities Al-Otibi, Fatimah Alshammry, Nourah A. Alharbi, Raedah I. Bin-Jumah, May N. AlSubaie, Maha M. Plants (Basel) Article Background: Artemisia sieberi (mugwort) is a member of the daisy family Asteraceae and is widely propagated in Saudi Arabia. A. sieberi has historical medical importance in traditional societies. The current study aimed to assess the antibacterial and antifungal characteristics of the aqueous and ethanolic extracts of A. sieberi. In addition, the study investigated the effect of silver nanoparticles (AgNPs) synthesized from the A. sieberi extract. Methods: The ethanolic and aqueous extracts and AgNPs were prepared from the shoots of A. sieberi. The characteristics of AgNPs were assessed by UV–visible spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS). The antibacterial experiments were performed against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. The fungal species used were Candida parapsilosis, Candida krusei, Candida famata, Candida rhodotorula, and Candida albicans. The antibacterial and antifungal characteristics were evaluated by measuring the diameter of growing organisms in Petri dishes treated with different concentrations of either extracts or AgNPs compared to the untreated controls. Furthermore, TEM imaging was used to investigate any ultrastructure changes in the microbes treated with crude extracts and AgNO(3). Results: The ethanolic and aqueous extracts significantly decreased the growth of E. coli, S. aureus, and B. subtilis (p < 0.001), while P. aeruginosa was not affected. Unlike crude extracts, AgNPs had more substantial antibacterial effects against all species. In addition, the mycelial growth of C. famata was reduced by the treatment of both extracts. C. krusei mycelial growth was decreased by the aqueous extract, while the growth of C. parapsilosis was affected by the ethanolic extract and AgNPs (p < 0.001). None of the treatments affected the growth of C. albicans or C. rhodotorula. TEM analysis showed cellular ultrastructure changes in the treated S. aureus and C. famata compared to the control. Conclusion: The biosynthesized AgNPs and extracts of A. sieberi have a potential antimicrobial characteristic against pathogenic bacterial and fungal strains and nullified resistance behavior. MDPI 2023-05-24 /pmc/articles/PMC10255823/ /pubmed/37299074 http://dx.doi.org/10.3390/plants12112093 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Otibi, Fatimah
Alshammry, Nourah A.
Alharbi, Raedah I.
Bin-Jumah, May N.
AlSubaie, Maha M.
Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities
title Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities
title_full Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities
title_fullStr Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities
title_full_unstemmed Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities
title_short Silver Nanoparticles of Artemisia sieberi Extracts: Chemical Composition and Antimicrobial Activities
title_sort silver nanoparticles of artemisia sieberi extracts: chemical composition and antimicrobial activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255823/
https://www.ncbi.nlm.nih.gov/pubmed/37299074
http://dx.doi.org/10.3390/plants12112093
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