Cargando…
Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato
Tomato yellow leaf curl disease (TYLCD) has become the key limiting factor for the production of tomato in many areas because of the continuous infection and recombination of several tomato yellow leaf curl virus (TYLCV)-like species (TYLCLV) which produce novel and destructive viruses. Artificial m...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255879/ https://www.ncbi.nlm.nih.gov/pubmed/37299158 http://dx.doi.org/10.3390/plants12112179 |
_version_ | 1785056979901743104 |
---|---|
author | Khalid, Annum Zhang, Xi Ji, Huaijin Yasir, Muhammad Farooq, Tariq Dai, Xinyi Li, Feng |
author_facet | Khalid, Annum Zhang, Xi Ji, Huaijin Yasir, Muhammad Farooq, Tariq Dai, Xinyi Li, Feng |
author_sort | Khalid, Annum |
collection | PubMed |
description | Tomato yellow leaf curl disease (TYLCD) has become the key limiting factor for the production of tomato in many areas because of the continuous infection and recombination of several tomato yellow leaf curl virus (TYLCV)-like species (TYLCLV) which produce novel and destructive viruses. Artificial microRNA (AMIR) is a recent and effective technology used to create viral resistance in major crops. This study applies AMIR technology in two ways, i.e., amiRNA in introns (AMINs) and amiRNA in exons (AMIEs), to express 14 amiRNAs targeting conserved regions in seven TYLCLV genes and their satellite DNA. The resulting pAMIN14 and pAMIE14 vectors can encode large AMIR clusters and their function in silencing reporter genes was validated with transient assays and stable transgenic N. tabacum plants. To assess the efficacy of conferring resistance against TYLCLV, pAMIE14 and pAMIN14 were transformed into tomato cultivar A57 and the resulting transgenic tomato plants were evaluated for their level of resistance to mixed TYLCLV infection. The results suggest that pAMIN14 transgenic lines have a more effective resistance than pAMIE14 transgenic lines, reaching a resistance level comparable to plants carrying the TY1 resistance gene. |
format | Online Article Text |
id | pubmed-10255879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102558792023-06-10 Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato Khalid, Annum Zhang, Xi Ji, Huaijin Yasir, Muhammad Farooq, Tariq Dai, Xinyi Li, Feng Plants (Basel) Article Tomato yellow leaf curl disease (TYLCD) has become the key limiting factor for the production of tomato in many areas because of the continuous infection and recombination of several tomato yellow leaf curl virus (TYLCV)-like species (TYLCLV) which produce novel and destructive viruses. Artificial microRNA (AMIR) is a recent and effective technology used to create viral resistance in major crops. This study applies AMIR technology in two ways, i.e., amiRNA in introns (AMINs) and amiRNA in exons (AMIEs), to express 14 amiRNAs targeting conserved regions in seven TYLCLV genes and their satellite DNA. The resulting pAMIN14 and pAMIE14 vectors can encode large AMIR clusters and their function in silencing reporter genes was validated with transient assays and stable transgenic N. tabacum plants. To assess the efficacy of conferring resistance against TYLCLV, pAMIE14 and pAMIN14 were transformed into tomato cultivar A57 and the resulting transgenic tomato plants were evaluated for their level of resistance to mixed TYLCLV infection. The results suggest that pAMIN14 transgenic lines have a more effective resistance than pAMIE14 transgenic lines, reaching a resistance level comparable to plants carrying the TY1 resistance gene. MDPI 2023-05-31 /pmc/articles/PMC10255879/ /pubmed/37299158 http://dx.doi.org/10.3390/plants12112179 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Khalid, Annum Zhang, Xi Ji, Huaijin Yasir, Muhammad Farooq, Tariq Dai, Xinyi Li, Feng Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato |
title | Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato |
title_full | Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato |
title_fullStr | Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato |
title_full_unstemmed | Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato |
title_short | Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato |
title_sort | large artificial microrna cluster genes confer effective resistance against multiple tomato yellow leaf curl viruses in transgenic tomato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255879/ https://www.ncbi.nlm.nih.gov/pubmed/37299158 http://dx.doi.org/10.3390/plants12112179 |
work_keys_str_mv | AT khalidannum largeartificialmicrornaclustergenesconfereffectiveresistanceagainstmultipletomatoyellowleafcurlvirusesintransgenictomato AT zhangxi largeartificialmicrornaclustergenesconfereffectiveresistanceagainstmultipletomatoyellowleafcurlvirusesintransgenictomato AT jihuaijin largeartificialmicrornaclustergenesconfereffectiveresistanceagainstmultipletomatoyellowleafcurlvirusesintransgenictomato AT yasirmuhammad largeartificialmicrornaclustergenesconfereffectiveresistanceagainstmultipletomatoyellowleafcurlvirusesintransgenictomato AT farooqtariq largeartificialmicrornaclustergenesconfereffectiveresistanceagainstmultipletomatoyellowleafcurlvirusesintransgenictomato AT daixinyi largeartificialmicrornaclustergenesconfereffectiveresistanceagainstmultipletomatoyellowleafcurlvirusesintransgenictomato AT lifeng largeartificialmicrornaclustergenesconfereffectiveresistanceagainstmultipletomatoyellowleafcurlvirusesintransgenictomato |