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Role of NPR1 in Systemic Acquired Stomatal Immunity
Stomatal immunity is the primary gate of the plant pathogen defense system. Non-expressor of Pathogenesis Related 1 (NPR1) is the salicylic acid (SA) receptor, which is critical for stomatal defense. SA induces stomatal closure, but the specific role of NPR1 in guard cells and its contribution to sy...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255907/ https://www.ncbi.nlm.nih.gov/pubmed/37299116 http://dx.doi.org/10.3390/plants12112137 |
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author | Guan, Qijie David, Lisa Moran, Riley Grela, Ivan Ortega, Angelica Scott, Peter Warnock, Lindsey Chen, Sixue |
author_facet | Guan, Qijie David, Lisa Moran, Riley Grela, Ivan Ortega, Angelica Scott, Peter Warnock, Lindsey Chen, Sixue |
author_sort | Guan, Qijie |
collection | PubMed |
description | Stomatal immunity is the primary gate of the plant pathogen defense system. Non-expressor of Pathogenesis Related 1 (NPR1) is the salicylic acid (SA) receptor, which is critical for stomatal defense. SA induces stomatal closure, but the specific role of NPR1 in guard cells and its contribution to systemic acquired resistance (SAR) remain largely unknown. In this study, we compared the response to pathogen attack in wild-type Arabidopsis and the npr1-1 knockout mutant in terms of stomatal movement and proteomic changes. We found that NPR1 does not regulate stomatal density, but the npr1-1 mutant failed to close stomata when under pathogen attack, resulting in more pathogens entering the leaves. Moreover, the ROS levels in the npr1-1 mutant were higher than in the wild type, and several proteins involved in carbon fixation, oxidative phosphorylation, glycolysis, and glutathione metabolism were differentially changed in abundance. Our findings suggest that mobile SAR signals alter stomatal immune response possibly by initiating ROS burst, and the npr1-1 mutant has an alternative priming effect through translational regulation. |
format | Online Article Text |
id | pubmed-10255907 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102559072023-06-10 Role of NPR1 in Systemic Acquired Stomatal Immunity Guan, Qijie David, Lisa Moran, Riley Grela, Ivan Ortega, Angelica Scott, Peter Warnock, Lindsey Chen, Sixue Plants (Basel) Article Stomatal immunity is the primary gate of the plant pathogen defense system. Non-expressor of Pathogenesis Related 1 (NPR1) is the salicylic acid (SA) receptor, which is critical for stomatal defense. SA induces stomatal closure, but the specific role of NPR1 in guard cells and its contribution to systemic acquired resistance (SAR) remain largely unknown. In this study, we compared the response to pathogen attack in wild-type Arabidopsis and the npr1-1 knockout mutant in terms of stomatal movement and proteomic changes. We found that NPR1 does not regulate stomatal density, but the npr1-1 mutant failed to close stomata when under pathogen attack, resulting in more pathogens entering the leaves. Moreover, the ROS levels in the npr1-1 mutant were higher than in the wild type, and several proteins involved in carbon fixation, oxidative phosphorylation, glycolysis, and glutathione metabolism were differentially changed in abundance. Our findings suggest that mobile SAR signals alter stomatal immune response possibly by initiating ROS burst, and the npr1-1 mutant has an alternative priming effect through translational regulation. MDPI 2023-05-29 /pmc/articles/PMC10255907/ /pubmed/37299116 http://dx.doi.org/10.3390/plants12112137 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guan, Qijie David, Lisa Moran, Riley Grela, Ivan Ortega, Angelica Scott, Peter Warnock, Lindsey Chen, Sixue Role of NPR1 in Systemic Acquired Stomatal Immunity |
title | Role of NPR1 in Systemic Acquired Stomatal Immunity |
title_full | Role of NPR1 in Systemic Acquired Stomatal Immunity |
title_fullStr | Role of NPR1 in Systemic Acquired Stomatal Immunity |
title_full_unstemmed | Role of NPR1 in Systemic Acquired Stomatal Immunity |
title_short | Role of NPR1 in Systemic Acquired Stomatal Immunity |
title_sort | role of npr1 in systemic acquired stomatal immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10255907/ https://www.ncbi.nlm.nih.gov/pubmed/37299116 http://dx.doi.org/10.3390/plants12112137 |
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