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Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS

In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawba...

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Autores principales: De Spiegeleer, Margot, Plekhova, Vera, Geltmeyer, Jozefien, Schoolaert, Ella, Pomian, Beata, Singh, Varoon, Wijnant, Kathleen, De Windt, Kimberly, Paukku, Volter, De Loof, Alexander, Gies, Inge, Michels, Nathalie, De Henauw, Stefaan, De Graeve, Marilyn, De Clerck, Karen, Vanhaecke, Lynn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256167/
https://www.ncbi.nlm.nih.gov/pubmed/37294759
http://dx.doi.org/10.1126/sciadv.ade9933
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author De Spiegeleer, Margot
Plekhova, Vera
Geltmeyer, Jozefien
Schoolaert, Ella
Pomian, Beata
Singh, Varoon
Wijnant, Kathleen
De Windt, Kimberly
Paukku, Volter
De Loof, Alexander
Gies, Inge
Michels, Nathalie
De Henauw, Stefaan
De Graeve, Marilyn
De Clerck, Karen
Vanhaecke, Lynn
author_facet De Spiegeleer, Margot
Plekhova, Vera
Geltmeyer, Jozefien
Schoolaert, Ella
Pomian, Beata
Singh, Varoon
Wijnant, Kathleen
De Windt, Kimberly
Paukku, Volter
De Loof, Alexander
Gies, Inge
Michels, Nathalie
De Henauw, Stefaan
De Graeve, Marilyn
De Clerck, Karen
Vanhaecke, Lynn
author_sort De Spiegeleer, Margot
collection PubMed
description In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AIMS. Customized rectal, salivary, and urinary MetaSAMPs consisting of electrospun (nano)fibrous membranes of blended hydrophilic (polyvinylpyrrolidone and polyacrylonitrile) and lipophilic (polystyrene) polymers supported metabolite absorption, adsorption, and desorption. Moreover, MetaSAMP demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis and was successfully validated in two pediatric cohorts (MetaBEAse, n = 234 and OPERA, n = 101). By integrating anthropometric and (patho)physiological with MetaSAMP-AIMS metabolome data, we obtained substantial weight-driven predictions and clinical correlations. In conclusion, MetaSAMP holds great clinical application potential for on-the-spot metabolic health stratification.
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spelling pubmed-102561672023-06-10 Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS De Spiegeleer, Margot Plekhova, Vera Geltmeyer, Jozefien Schoolaert, Ella Pomian, Beata Singh, Varoon Wijnant, Kathleen De Windt, Kimberly Paukku, Volter De Loof, Alexander Gies, Inge Michels, Nathalie De Henauw, Stefaan De Graeve, Marilyn De Clerck, Karen Vanhaecke, Lynn Sci Adv Biomedicine and Life Sciences In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AIMS. Customized rectal, salivary, and urinary MetaSAMPs consisting of electrospun (nano)fibrous membranes of blended hydrophilic (polyvinylpyrrolidone and polyacrylonitrile) and lipophilic (polystyrene) polymers supported metabolite absorption, adsorption, and desorption. Moreover, MetaSAMP demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis and was successfully validated in two pediatric cohorts (MetaBEAse, n = 234 and OPERA, n = 101). By integrating anthropometric and (patho)physiological with MetaSAMP-AIMS metabolome data, we obtained substantial weight-driven predictions and clinical correlations. In conclusion, MetaSAMP holds great clinical application potential for on-the-spot metabolic health stratification. American Association for the Advancement of Science 2023-06-09 /pmc/articles/PMC10256167/ /pubmed/37294759 http://dx.doi.org/10.1126/sciadv.ade9933 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
De Spiegeleer, Margot
Plekhova, Vera
Geltmeyer, Jozefien
Schoolaert, Ella
Pomian, Beata
Singh, Varoon
Wijnant, Kathleen
De Windt, Kimberly
Paukku, Volter
De Loof, Alexander
Gies, Inge
Michels, Nathalie
De Henauw, Stefaan
De Graeve, Marilyn
De Clerck, Karen
Vanhaecke, Lynn
Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS
title Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS
title_full Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS
title_fullStr Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS
title_full_unstemmed Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS
title_short Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS
title_sort point-of-care applicable metabotyping using biofluid-specific electrospun metasamps directly amenable to ambient la-reims
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256167/
https://www.ncbi.nlm.nih.gov/pubmed/37294759
http://dx.doi.org/10.1126/sciadv.ade9933
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