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Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS
In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawba...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256167/ https://www.ncbi.nlm.nih.gov/pubmed/37294759 http://dx.doi.org/10.1126/sciadv.ade9933 |
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author | De Spiegeleer, Margot Plekhova, Vera Geltmeyer, Jozefien Schoolaert, Ella Pomian, Beata Singh, Varoon Wijnant, Kathleen De Windt, Kimberly Paukku, Volter De Loof, Alexander Gies, Inge Michels, Nathalie De Henauw, Stefaan De Graeve, Marilyn De Clerck, Karen Vanhaecke, Lynn |
author_facet | De Spiegeleer, Margot Plekhova, Vera Geltmeyer, Jozefien Schoolaert, Ella Pomian, Beata Singh, Varoon Wijnant, Kathleen De Windt, Kimberly Paukku, Volter De Loof, Alexander Gies, Inge Michels, Nathalie De Henauw, Stefaan De Graeve, Marilyn De Clerck, Karen Vanhaecke, Lynn |
author_sort | De Spiegeleer, Margot |
collection | PubMed |
description | In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AIMS. Customized rectal, salivary, and urinary MetaSAMPs consisting of electrospun (nano)fibrous membranes of blended hydrophilic (polyvinylpyrrolidone and polyacrylonitrile) and lipophilic (polystyrene) polymers supported metabolite absorption, adsorption, and desorption. Moreover, MetaSAMP demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis and was successfully validated in two pediatric cohorts (MetaBEAse, n = 234 and OPERA, n = 101). By integrating anthropometric and (patho)physiological with MetaSAMP-AIMS metabolome data, we obtained substantial weight-driven predictions and clinical correlations. In conclusion, MetaSAMP holds great clinical application potential for on-the-spot metabolic health stratification. |
format | Online Article Text |
id | pubmed-10256167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-102561672023-06-10 Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS De Spiegeleer, Margot Plekhova, Vera Geltmeyer, Jozefien Schoolaert, Ella Pomian, Beata Singh, Varoon Wijnant, Kathleen De Windt, Kimberly Paukku, Volter De Loof, Alexander Gies, Inge Michels, Nathalie De Henauw, Stefaan De Graeve, Marilyn De Clerck, Karen Vanhaecke, Lynn Sci Adv Biomedicine and Life Sciences In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AIMS. Customized rectal, salivary, and urinary MetaSAMPs consisting of electrospun (nano)fibrous membranes of blended hydrophilic (polyvinylpyrrolidone and polyacrylonitrile) and lipophilic (polystyrene) polymers supported metabolite absorption, adsorption, and desorption. Moreover, MetaSAMP demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis and was successfully validated in two pediatric cohorts (MetaBEAse, n = 234 and OPERA, n = 101). By integrating anthropometric and (patho)physiological with MetaSAMP-AIMS metabolome data, we obtained substantial weight-driven predictions and clinical correlations. In conclusion, MetaSAMP holds great clinical application potential for on-the-spot metabolic health stratification. American Association for the Advancement of Science 2023-06-09 /pmc/articles/PMC10256167/ /pubmed/37294759 http://dx.doi.org/10.1126/sciadv.ade9933 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences De Spiegeleer, Margot Plekhova, Vera Geltmeyer, Jozefien Schoolaert, Ella Pomian, Beata Singh, Varoon Wijnant, Kathleen De Windt, Kimberly Paukku, Volter De Loof, Alexander Gies, Inge Michels, Nathalie De Henauw, Stefaan De Graeve, Marilyn De Clerck, Karen Vanhaecke, Lynn Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS |
title | Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS |
title_full | Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS |
title_fullStr | Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS |
title_full_unstemmed | Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS |
title_short | Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS |
title_sort | point-of-care applicable metabotyping using biofluid-specific electrospun metasamps directly amenable to ambient la-reims |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256167/ https://www.ncbi.nlm.nih.gov/pubmed/37294759 http://dx.doi.org/10.1126/sciadv.ade9933 |
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