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Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and asses...

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Autores principales: Rungrojcharoenkit, Kamonthip, Suthangkornkul, Rungarun, Utennam, Darunee, Buddhari, Darunee, Pinpaiboon, Soontorn, Mongkolsirichaikul, Duangrat, Fernandez, Stefan, Jones, Anthony R., Cotrone, Thomas S., Hunsawong, Taweewun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256204/
https://www.ncbi.nlm.nih.gov/pubmed/37294808
http://dx.doi.org/10.1371/journal.pone.0287107
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author Rungrojcharoenkit, Kamonthip
Suthangkornkul, Rungarun
Utennam, Darunee
Buddhari, Darunee
Pinpaiboon, Soontorn
Mongkolsirichaikul, Duangrat
Fernandez, Stefan
Jones, Anthony R.
Cotrone, Thomas S.
Hunsawong, Taweewun
author_facet Rungrojcharoenkit, Kamonthip
Suthangkornkul, Rungarun
Utennam, Darunee
Buddhari, Darunee
Pinpaiboon, Soontorn
Mongkolsirichaikul, Duangrat
Fernandez, Stefan
Jones, Anthony R.
Cotrone, Thomas S.
Hunsawong, Taweewun
author_sort Rungrojcharoenkit, Kamonthip
collection PubMed
description Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection.
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spelling pubmed-102562042023-06-10 Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19 Rungrojcharoenkit, Kamonthip Suthangkornkul, Rungarun Utennam, Darunee Buddhari, Darunee Pinpaiboon, Soontorn Mongkolsirichaikul, Duangrat Fernandez, Stefan Jones, Anthony R. Cotrone, Thomas S. Hunsawong, Taweewun PLoS One Research Article Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection. Public Library of Science 2023-06-09 /pmc/articles/PMC10256204/ /pubmed/37294808 http://dx.doi.org/10.1371/journal.pone.0287107 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Rungrojcharoenkit, Kamonthip
Suthangkornkul, Rungarun
Utennam, Darunee
Buddhari, Darunee
Pinpaiboon, Soontorn
Mongkolsirichaikul, Duangrat
Fernandez, Stefan
Jones, Anthony R.
Cotrone, Thomas S.
Hunsawong, Taweewun
Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
title Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
title_full Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
title_fullStr Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
title_full_unstemmed Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
title_short Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
title_sort standardization of in-house anti-igg and iga elisas for the detection of covid-19
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256204/
https://www.ncbi.nlm.nih.gov/pubmed/37294808
http://dx.doi.org/10.1371/journal.pone.0287107
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