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Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and asses...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256204/ https://www.ncbi.nlm.nih.gov/pubmed/37294808 http://dx.doi.org/10.1371/journal.pone.0287107 |
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author | Rungrojcharoenkit, Kamonthip Suthangkornkul, Rungarun Utennam, Darunee Buddhari, Darunee Pinpaiboon, Soontorn Mongkolsirichaikul, Duangrat Fernandez, Stefan Jones, Anthony R. Cotrone, Thomas S. Hunsawong, Taweewun |
author_facet | Rungrojcharoenkit, Kamonthip Suthangkornkul, Rungarun Utennam, Darunee Buddhari, Darunee Pinpaiboon, Soontorn Mongkolsirichaikul, Duangrat Fernandez, Stefan Jones, Anthony R. Cotrone, Thomas S. Hunsawong, Taweewun |
author_sort | Rungrojcharoenkit, Kamonthip |
collection | PubMed |
description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection. |
format | Online Article Text |
id | pubmed-10256204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-102562042023-06-10 Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19 Rungrojcharoenkit, Kamonthip Suthangkornkul, Rungarun Utennam, Darunee Buddhari, Darunee Pinpaiboon, Soontorn Mongkolsirichaikul, Duangrat Fernandez, Stefan Jones, Anthony R. Cotrone, Thomas S. Hunsawong, Taweewun PLoS One Research Article Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection. Public Library of Science 2023-06-09 /pmc/articles/PMC10256204/ /pubmed/37294808 http://dx.doi.org/10.1371/journal.pone.0287107 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Rungrojcharoenkit, Kamonthip Suthangkornkul, Rungarun Utennam, Darunee Buddhari, Darunee Pinpaiboon, Soontorn Mongkolsirichaikul, Duangrat Fernandez, Stefan Jones, Anthony R. Cotrone, Thomas S. Hunsawong, Taweewun Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19 |
title | Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19 |
title_full | Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19 |
title_fullStr | Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19 |
title_full_unstemmed | Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19 |
title_short | Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19 |
title_sort | standardization of in-house anti-igg and iga elisas for the detection of covid-19 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256204/ https://www.ncbi.nlm.nih.gov/pubmed/37294808 http://dx.doi.org/10.1371/journal.pone.0287107 |
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