Wastewater surveillance of Mpox virus in Baltimore

This study aimed to utilize wastewater surveillance for monitoring Mpox cases at a community level. Untreated wastewater samples were collected once a week from two wastewater treatment plants (A and B) in Baltimore City from July 27, 2022–September 22, 2022. The samples were concentrated via an ads...

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Detalles Bibliográficos
Autores principales: Sherchan, Samendra P., Solomon, Tamunobelema, Idris, Oladele, Nwaubani, Daniel, Thakali, Ocean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256456/
https://www.ncbi.nlm.nih.gov/pubmed/37230346
http://dx.doi.org/10.1016/j.scitotenv.2023.164414
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author Sherchan, Samendra P.
Solomon, Tamunobelema
Idris, Oladele
Nwaubani, Daniel
Thakali, Ocean
author_facet Sherchan, Samendra P.
Solomon, Tamunobelema
Idris, Oladele
Nwaubani, Daniel
Thakali, Ocean
author_sort Sherchan, Samendra P.
collection PubMed
description This study aimed to utilize wastewater surveillance for monitoring Mpox cases at a community level. Untreated wastewater samples were collected once a week from two wastewater treatment plants (A and B) in Baltimore City from July 27, 2022–September 22, 2022. The samples were concentrated via an adsorption–elution (AE) method and Polyethylene Glycol (PEG) precipitation method followed by quantitative polymerase chain reaction (qPCR). Monkeypox virus (MPXV) was detected in 89 % (8/9) samples from WWTP A and 55 % (5/9) samples from WWTP B with at least one concentration method. Higher detection rate in samples concentrated with PEG precipitation compared to AE method was observed, indicating that PEG precipitation is a more effective virus concentration method for MPXV. To our knowledge, this is the first study reporting the detection of MPXV in wastewater in Baltimore. The results highlight that wastewater surveillance could be used as a complementary early warning tool for monitoring future Mpox outbreaks.
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spelling pubmed-102564562023-06-10 Wastewater surveillance of Mpox virus in Baltimore Sherchan, Samendra P. Solomon, Tamunobelema Idris, Oladele Nwaubani, Daniel Thakali, Ocean Sci Total Environ Short Communication This study aimed to utilize wastewater surveillance for monitoring Mpox cases at a community level. Untreated wastewater samples were collected once a week from two wastewater treatment plants (A and B) in Baltimore City from July 27, 2022–September 22, 2022. The samples were concentrated via an adsorption–elution (AE) method and Polyethylene Glycol (PEG) precipitation method followed by quantitative polymerase chain reaction (qPCR). Monkeypox virus (MPXV) was detected in 89 % (8/9) samples from WWTP A and 55 % (5/9) samples from WWTP B with at least one concentration method. Higher detection rate in samples concentrated with PEG precipitation compared to AE method was observed, indicating that PEG precipitation is a more effective virus concentration method for MPXV. To our knowledge, this is the first study reporting the detection of MPXV in wastewater in Baltimore. The results highlight that wastewater surveillance could be used as a complementary early warning tool for monitoring future Mpox outbreaks. Published by Elsevier B.V. 2023-09-15 2023-05-23 /pmc/articles/PMC10256456/ /pubmed/37230346 http://dx.doi.org/10.1016/j.scitotenv.2023.164414 Text en © 2023 Published by Elsevier B.V. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active.
spellingShingle Short Communication
Sherchan, Samendra P.
Solomon, Tamunobelema
Idris, Oladele
Nwaubani, Daniel
Thakali, Ocean
Wastewater surveillance of Mpox virus in Baltimore
title Wastewater surveillance of Mpox virus in Baltimore
title_full Wastewater surveillance of Mpox virus in Baltimore
title_fullStr Wastewater surveillance of Mpox virus in Baltimore
title_full_unstemmed Wastewater surveillance of Mpox virus in Baltimore
title_short Wastewater surveillance of Mpox virus in Baltimore
title_sort wastewater surveillance of mpox virus in baltimore
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256456/
https://www.ncbi.nlm.nih.gov/pubmed/37230346
http://dx.doi.org/10.1016/j.scitotenv.2023.164414
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