Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy

The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as periv...

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Detalles Bibliográficos
Autores principales: Urata, Shinji, Okabe, Shigeo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2023
Materias:
Method Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256671/
https://www.ncbi.nlm.nih.gov/pubmed/36773194
http://dx.doi.org/10.1007/s12565-023-00703-z
Descripción
Sumario:The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1(+/GFP) mice. This study’s method was effective in elucidating inner ear function under both physiological and pathological conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12565-023-00703-z.

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