Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy

The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as periv...

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Autores principales: Urata, Shinji, Okabe, Shigeo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2023
Materias:
Method Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256671/
https://www.ncbi.nlm.nih.gov/pubmed/36773194
http://dx.doi.org/10.1007/s12565-023-00703-z
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author Urata, Shinji
Okabe, Shigeo
author_facet Urata, Shinji
Okabe, Shigeo
author_sort Urata, Shinji
collection PubMed
description The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1(+/GFP) mice. This study’s method was effective in elucidating inner ear function under both physiological and pathological conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12565-023-00703-z.
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spelling pubmed-102566712023-06-11 Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy Urata, Shinji Okabe, Shigeo Anat Sci Int Method Paper The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1(+/GFP) mice. This study’s method was effective in elucidating inner ear function under both physiological and pathological conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12565-023-00703-z. Springer Nature Singapore 2023-02-11 2023 /pmc/articles/PMC10256671/ /pubmed/36773194 http://dx.doi.org/10.1007/s12565-023-00703-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Method Paper
Urata, Shinji
Okabe, Shigeo
Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy
title Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy
title_full Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy
title_fullStr Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy
title_full_unstemmed Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy
title_short Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy
title_sort three-dimensional mouse cochlea imaging based on the modified sca/es using confocal microscopy
topic Method Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10256671/
https://www.ncbi.nlm.nih.gov/pubmed/36773194
http://dx.doi.org/10.1007/s12565-023-00703-z
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AT okabeshigeo threedimensionalmousecochleaimagingbasedonthemodifiedscaesusingconfocalmicroscopy

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