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Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota

BACKGROUND: The structure of gut microbiota is highly complex. Insects have ubiquitous associations with intestinal symbiotic bacteria, which play essential roles. Thus, understanding how changes in the abundance of a single bacterium interfere with bacterial interactions in the insect’s gut is impo...

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Autores principales: Li, Ying, Wang, Shumin, Zhang, Kexin, Yin, Yansong, Zhang, Xinyu, Zhang, Qian, Kong, Xinxin, Tang, Luyao, Zhang, Ruiling, Zhang, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257315/
https://www.ncbi.nlm.nih.gov/pubmed/37301969
http://dx.doi.org/10.1186/s13071-023-05781-6
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author Li, Ying
Wang, Shumin
Zhang, Kexin
Yin, Yansong
Zhang, Xinyu
Zhang, Qian
Kong, Xinxin
Tang, Luyao
Zhang, Ruiling
Zhang, Zhong
author_facet Li, Ying
Wang, Shumin
Zhang, Kexin
Yin, Yansong
Zhang, Xinyu
Zhang, Qian
Kong, Xinxin
Tang, Luyao
Zhang, Ruiling
Zhang, Zhong
author_sort Li, Ying
collection PubMed
description BACKGROUND: The structure of gut microbiota is highly complex. Insects have ubiquitous associations with intestinal symbiotic bacteria, which play essential roles. Thus, understanding how changes in the abundance of a single bacterium interfere with bacterial interactions in the insect’s gut is important. METHODS: Here, we analyzed the effects of Serratia marcescens on the growth and development of housefly larvae using phage technology. We used 16S rRNA gene sequencing technology to explore dynamic diversity and variation in gut bacterial communities and performed plate confrontation assays to study the interaction between S. marcescens and intestinal microorganisms. Furthermore, we performed phenoloxidase activity assay, crawling assay, and trypan blue staining to explore the negative effects of S. marcescens on housefly larvae’s humoral immunity, motility, and intestinal organization. RESULTS: The growth and development of housefly larvae were inhibited after feeding on S. marcescens, and their intestinal bacterial composition changed with increasing abundance of Providencia and decreasing abundance of Enterobacter and Klebsiella. Meanwhile, the depletion of S. marcescens by phages promoted the reproduction of beneficial bacteria. CONCLUSIONS: In our study, using phage as a tool to regulate the abundance of S. marcescens, we highlighted the mechanism by which S. marcescens inhibits the growth and development of housefly larvae and illustrated the importance of intestinal flora for larval development. Furthermore, by studying the dynamic diversity and variation in gut bacterial communities, we improved our understanding of the possible relationship between the gut microbiome and housefly larvae when houseflies are invaded by exogenous pathogenic bacteria. GRAPHICAL ABSTRACT: SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05781-6.
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spelling pubmed-102573152023-06-11 Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota Li, Ying Wang, Shumin Zhang, Kexin Yin, Yansong Zhang, Xinyu Zhang, Qian Kong, Xinxin Tang, Luyao Zhang, Ruiling Zhang, Zhong Parasit Vectors Research BACKGROUND: The structure of gut microbiota is highly complex. Insects have ubiquitous associations with intestinal symbiotic bacteria, which play essential roles. Thus, understanding how changes in the abundance of a single bacterium interfere with bacterial interactions in the insect’s gut is important. METHODS: Here, we analyzed the effects of Serratia marcescens on the growth and development of housefly larvae using phage technology. We used 16S rRNA gene sequencing technology to explore dynamic diversity and variation in gut bacterial communities and performed plate confrontation assays to study the interaction between S. marcescens and intestinal microorganisms. Furthermore, we performed phenoloxidase activity assay, crawling assay, and trypan blue staining to explore the negative effects of S. marcescens on housefly larvae’s humoral immunity, motility, and intestinal organization. RESULTS: The growth and development of housefly larvae were inhibited after feeding on S. marcescens, and their intestinal bacterial composition changed with increasing abundance of Providencia and decreasing abundance of Enterobacter and Klebsiella. Meanwhile, the depletion of S. marcescens by phages promoted the reproduction of beneficial bacteria. CONCLUSIONS: In our study, using phage as a tool to regulate the abundance of S. marcescens, we highlighted the mechanism by which S. marcescens inhibits the growth and development of housefly larvae and illustrated the importance of intestinal flora for larval development. Furthermore, by studying the dynamic diversity and variation in gut bacterial communities, we improved our understanding of the possible relationship between the gut microbiome and housefly larvae when houseflies are invaded by exogenous pathogenic bacteria. GRAPHICAL ABSTRACT: SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05781-6. BioMed Central 2023-06-10 /pmc/articles/PMC10257315/ /pubmed/37301969 http://dx.doi.org/10.1186/s13071-023-05781-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Ying
Wang, Shumin
Zhang, Kexin
Yin, Yansong
Zhang, Xinyu
Zhang, Qian
Kong, Xinxin
Tang, Luyao
Zhang, Ruiling
Zhang, Zhong
Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota
title Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota
title_full Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota
title_fullStr Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota
title_full_unstemmed Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota
title_short Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota
title_sort serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257315/
https://www.ncbi.nlm.nih.gov/pubmed/37301969
http://dx.doi.org/10.1186/s13071-023-05781-6
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