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Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota
BACKGROUND: The structure of gut microbiota is highly complex. Insects have ubiquitous associations with intestinal symbiotic bacteria, which play essential roles. Thus, understanding how changes in the abundance of a single bacterium interfere with bacterial interactions in the insect’s gut is impo...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257315/ https://www.ncbi.nlm.nih.gov/pubmed/37301969 http://dx.doi.org/10.1186/s13071-023-05781-6 |
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author | Li, Ying Wang, Shumin Zhang, Kexin Yin, Yansong Zhang, Xinyu Zhang, Qian Kong, Xinxin Tang, Luyao Zhang, Ruiling Zhang, Zhong |
author_facet | Li, Ying Wang, Shumin Zhang, Kexin Yin, Yansong Zhang, Xinyu Zhang, Qian Kong, Xinxin Tang, Luyao Zhang, Ruiling Zhang, Zhong |
author_sort | Li, Ying |
collection | PubMed |
description | BACKGROUND: The structure of gut microbiota is highly complex. Insects have ubiquitous associations with intestinal symbiotic bacteria, which play essential roles. Thus, understanding how changes in the abundance of a single bacterium interfere with bacterial interactions in the insect’s gut is important. METHODS: Here, we analyzed the effects of Serratia marcescens on the growth and development of housefly larvae using phage technology. We used 16S rRNA gene sequencing technology to explore dynamic diversity and variation in gut bacterial communities and performed plate confrontation assays to study the interaction between S. marcescens and intestinal microorganisms. Furthermore, we performed phenoloxidase activity assay, crawling assay, and trypan blue staining to explore the negative effects of S. marcescens on housefly larvae’s humoral immunity, motility, and intestinal organization. RESULTS: The growth and development of housefly larvae were inhibited after feeding on S. marcescens, and their intestinal bacterial composition changed with increasing abundance of Providencia and decreasing abundance of Enterobacter and Klebsiella. Meanwhile, the depletion of S. marcescens by phages promoted the reproduction of beneficial bacteria. CONCLUSIONS: In our study, using phage as a tool to regulate the abundance of S. marcescens, we highlighted the mechanism by which S. marcescens inhibits the growth and development of housefly larvae and illustrated the importance of intestinal flora for larval development. Furthermore, by studying the dynamic diversity and variation in gut bacterial communities, we improved our understanding of the possible relationship between the gut microbiome and housefly larvae when houseflies are invaded by exogenous pathogenic bacteria. GRAPHICAL ABSTRACT: SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05781-6. |
format | Online Article Text |
id | pubmed-10257315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-102573152023-06-11 Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota Li, Ying Wang, Shumin Zhang, Kexin Yin, Yansong Zhang, Xinyu Zhang, Qian Kong, Xinxin Tang, Luyao Zhang, Ruiling Zhang, Zhong Parasit Vectors Research BACKGROUND: The structure of gut microbiota is highly complex. Insects have ubiquitous associations with intestinal symbiotic bacteria, which play essential roles. Thus, understanding how changes in the abundance of a single bacterium interfere with bacterial interactions in the insect’s gut is important. METHODS: Here, we analyzed the effects of Serratia marcescens on the growth and development of housefly larvae using phage technology. We used 16S rRNA gene sequencing technology to explore dynamic diversity and variation in gut bacterial communities and performed plate confrontation assays to study the interaction between S. marcescens and intestinal microorganisms. Furthermore, we performed phenoloxidase activity assay, crawling assay, and trypan blue staining to explore the negative effects of S. marcescens on housefly larvae’s humoral immunity, motility, and intestinal organization. RESULTS: The growth and development of housefly larvae were inhibited after feeding on S. marcescens, and their intestinal bacterial composition changed with increasing abundance of Providencia and decreasing abundance of Enterobacter and Klebsiella. Meanwhile, the depletion of S. marcescens by phages promoted the reproduction of beneficial bacteria. CONCLUSIONS: In our study, using phage as a tool to regulate the abundance of S. marcescens, we highlighted the mechanism by which S. marcescens inhibits the growth and development of housefly larvae and illustrated the importance of intestinal flora for larval development. Furthermore, by studying the dynamic diversity and variation in gut bacterial communities, we improved our understanding of the possible relationship between the gut microbiome and housefly larvae when houseflies are invaded by exogenous pathogenic bacteria. GRAPHICAL ABSTRACT: SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05781-6. BioMed Central 2023-06-10 /pmc/articles/PMC10257315/ /pubmed/37301969 http://dx.doi.org/10.1186/s13071-023-05781-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Li, Ying Wang, Shumin Zhang, Kexin Yin, Yansong Zhang, Xinyu Zhang, Qian Kong, Xinxin Tang, Luyao Zhang, Ruiling Zhang, Zhong Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota |
title | Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota |
title_full | Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota |
title_fullStr | Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota |
title_full_unstemmed | Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota |
title_short | Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota |
title_sort | serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257315/ https://www.ncbi.nlm.nih.gov/pubmed/37301969 http://dx.doi.org/10.1186/s13071-023-05781-6 |
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