Cargando…

An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells

Colorectal cancer is associated with significant morbidity and mortality worldwide. Egypt, as a developing country, has a high-rise incidence of cancer. The current study objective was to investigate the antitumor influences of ellagitannin-loaded CS-PEG-decorated PLGA nano-prototypes against human...

Descripción completa

Detalles Bibliográficos
Autores principales: Abd-Rabou, Ahmed A., Shalby, Aziza B., Kotob, Soheir E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257629/
https://www.ncbi.nlm.nih.gov/pubmed/37067762
http://dx.doi.org/10.1007/s12013-023-01132-5
_version_ 1785057340858302464
author Abd-Rabou, Ahmed A.
Shalby, Aziza B.
Kotob, Soheir E.
author_facet Abd-Rabou, Ahmed A.
Shalby, Aziza B.
Kotob, Soheir E.
author_sort Abd-Rabou, Ahmed A.
collection PubMed
description Colorectal cancer is associated with significant morbidity and mortality worldwide. Egypt, as a developing country, has a high-rise incidence of cancer. The current study objective was to investigate the antitumor influences of ellagitannin-loaded CS-PEG-decorated PLGA nano-prototypes against human colorectal cancer cell lines (HCT 116 as well as Caco-2) in vitro. Doxorubicin (DOX), punicalin (PN), and punicalagin (PNG)-encapsulated chitosan-polyethylene glycol-decorated PLGA (PLGA-CS-PEG) nanoparticles (NPs) were described. The cytotoxicity of each preparation was evaluated using MTT assays in HCT 116 as well as Caco-2 cells during G0, G1, S, and G2 cell cycle phases. Cell cycle-related gene expression and protein levels were measured after treatment. Reactive oxygen species (ROS) levels were also measured. Both PN and PNG PLGA-CS-PEG NPs induce colon cancer cell death with cell cycle arrest in the G1 phase in vitro. Caco-2 cells were more sensitive to the nano-therapy than HCT 116 cells. Upon treatment, the ratio of Bax to Bcl-2 expression was increased following nano-therapy, with increased levels of Cas-3 and decreased expression of Bcl-2, PI3k, and NF-ĸB compared to control. The nitric oxide level (NO), a marker of ROS, was increased following nano-therapy compared to control. In conclusion, ROS-mediated cell cycle arrest can be induced by PN as well as PNG nano-therapy in cell lines of colorectal cancer.
format Online
Article
Text
id pubmed-10257629
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-102576292023-06-12 An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells Abd-Rabou, Ahmed A. Shalby, Aziza B. Kotob, Soheir E. Cell Biochem Biophys Original Paper Colorectal cancer is associated with significant morbidity and mortality worldwide. Egypt, as a developing country, has a high-rise incidence of cancer. The current study objective was to investigate the antitumor influences of ellagitannin-loaded CS-PEG-decorated PLGA nano-prototypes against human colorectal cancer cell lines (HCT 116 as well as Caco-2) in vitro. Doxorubicin (DOX), punicalin (PN), and punicalagin (PNG)-encapsulated chitosan-polyethylene glycol-decorated PLGA (PLGA-CS-PEG) nanoparticles (NPs) were described. The cytotoxicity of each preparation was evaluated using MTT assays in HCT 116 as well as Caco-2 cells during G0, G1, S, and G2 cell cycle phases. Cell cycle-related gene expression and protein levels were measured after treatment. Reactive oxygen species (ROS) levels were also measured. Both PN and PNG PLGA-CS-PEG NPs induce colon cancer cell death with cell cycle arrest in the G1 phase in vitro. Caco-2 cells were more sensitive to the nano-therapy than HCT 116 cells. Upon treatment, the ratio of Bax to Bcl-2 expression was increased following nano-therapy, with increased levels of Cas-3 and decreased expression of Bcl-2, PI3k, and NF-ĸB compared to control. The nitric oxide level (NO), a marker of ROS, was increased following nano-therapy compared to control. In conclusion, ROS-mediated cell cycle arrest can be induced by PN as well as PNG nano-therapy in cell lines of colorectal cancer. Springer US 2023-04-17 2023 /pmc/articles/PMC10257629/ /pubmed/37067762 http://dx.doi.org/10.1007/s12013-023-01132-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Abd-Rabou, Ahmed A.
Shalby, Aziza B.
Kotob, Soheir E.
An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells
title An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells
title_full An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells
title_fullStr An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells
title_full_unstemmed An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells
title_short An ellagitannin-loaded CS-PEG decorated PLGA nano-prototype promotes cell cycle arrest in colorectal cancer cells
title_sort ellagitannin-loaded cs-peg decorated plga nano-prototype promotes cell cycle arrest in colorectal cancer cells
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257629/
https://www.ncbi.nlm.nih.gov/pubmed/37067762
http://dx.doi.org/10.1007/s12013-023-01132-5
work_keys_str_mv AT abdrabouahmeda anellagitanninloadedcspegdecoratedplgananoprototypepromotescellcyclearrestincolorectalcancercells
AT shalbyazizab anellagitanninloadedcspegdecoratedplgananoprototypepromotescellcyclearrestincolorectalcancercells
AT kotobsoheire anellagitanninloadedcspegdecoratedplgananoprototypepromotescellcyclearrestincolorectalcancercells
AT abdrabouahmeda ellagitanninloadedcspegdecoratedplgananoprototypepromotescellcyclearrestincolorectalcancercells
AT shalbyazizab ellagitanninloadedcspegdecoratedplgananoprototypepromotescellcyclearrestincolorectalcancercells
AT kotobsoheire ellagitanninloadedcspegdecoratedplgananoprototypepromotescellcyclearrestincolorectalcancercells