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Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis

Ulcerative colitis (UC), one of the inflammatory bowel diseases, has been reported to increase in recent years. Although the exact cause is unknown, disruptions in the molecular pathways are thought to trigger UC. We aimed to examine the distributions of glycogen synthase kinase-3beta (GSK-3β), nucl...

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Autores principales: Ozbey, Caner, Aydemir, Isil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257782/
https://www.ncbi.nlm.nih.gov/pubmed/37128792
http://dx.doi.org/10.47162/RJME.64.1.07
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author Ozbey, Caner
Aydemir, Isil
author_facet Ozbey, Caner
Aydemir, Isil
author_sort Ozbey, Caner
collection PubMed
description Ulcerative colitis (UC), one of the inflammatory bowel diseases, has been reported to increase in recent years. Although the exact cause is unknown, disruptions in the molecular pathways are thought to trigger UC. We aimed to examine the distributions of glycogen synthase kinase-3beta (GSK-3β), nuclear factor-kappa B (NF-κB) and wingless/int-1 (Wnt-1) in different age groups diagnosed with UC. Patients diagnosed with UC were divided into four groups according to their ages: Group 1, aged 18–30 (n=20); Group 2, aged 31–45 (n=20); Group 3, aged 46–60 (n=20); Group 4, aged 61–75 (n=20). Tissue sections were histochemically stained to examine the parameters of epithelial cell height, length of crypt, thickness of muscularis mucosa and extent of submucosal fibrosis. The immunohistochemistry assay was performed using cell survival and for GSK-3β, NF-κB and Wnt-1 cell growth markers. Immunoreactivities were evaluated using H-score and analyzed using the one-way analysis of variance (ANOVA) test for statistics. It was detected a decrease in the histopathological parameters whereas the immunoreactivities of GSK-3β, NF-κB and Wnt-1 were increased with increasing age. The levels of GSK-3β immunoreactivity were similar in both epithelium and submucosa in all groups. NF-κB immunoreactivity was higher in submucosa of Groups 1, 2 and 3, while Wnt-1 was enhanced in Groups 1 and 3. The results of histopathology showed that the integrity of the epithelial tissue in the colon deteriorated with increasing age. The expressions of GSK-3β, NF-κB and Wnt-1 were detected in all age groups. We thought that there was a synergistic activation between these three markers. Nevertheless, studies are needed to investigate this molecular pathway.
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spelling pubmed-102577822023-06-12 Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis Ozbey, Caner Aydemir, Isil Rom J Morphol Embryol Original Paper Ulcerative colitis (UC), one of the inflammatory bowel diseases, has been reported to increase in recent years. Although the exact cause is unknown, disruptions in the molecular pathways are thought to trigger UC. We aimed to examine the distributions of glycogen synthase kinase-3beta (GSK-3β), nuclear factor-kappa B (NF-κB) and wingless/int-1 (Wnt-1) in different age groups diagnosed with UC. Patients diagnosed with UC were divided into four groups according to their ages: Group 1, aged 18–30 (n=20); Group 2, aged 31–45 (n=20); Group 3, aged 46–60 (n=20); Group 4, aged 61–75 (n=20). Tissue sections were histochemically stained to examine the parameters of epithelial cell height, length of crypt, thickness of muscularis mucosa and extent of submucosal fibrosis. The immunohistochemistry assay was performed using cell survival and for GSK-3β, NF-κB and Wnt-1 cell growth markers. Immunoreactivities were evaluated using H-score and analyzed using the one-way analysis of variance (ANOVA) test for statistics. It was detected a decrease in the histopathological parameters whereas the immunoreactivities of GSK-3β, NF-κB and Wnt-1 were increased with increasing age. The levels of GSK-3β immunoreactivity were similar in both epithelium and submucosa in all groups. NF-κB immunoreactivity was higher in submucosa of Groups 1, 2 and 3, while Wnt-1 was enhanced in Groups 1 and 3. The results of histopathology showed that the integrity of the epithelial tissue in the colon deteriorated with increasing age. The expressions of GSK-3β, NF-κB and Wnt-1 were detected in all age groups. We thought that there was a synergistic activation between these three markers. Nevertheless, studies are needed to investigate this molecular pathway. Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest 2023 2023-03-31 /pmc/articles/PMC10257782/ /pubmed/37128792 http://dx.doi.org/10.47162/RJME.64.1.07 Text en Copyright © 2023, Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License, which permits unrestricted use, adaptation, distribution and reproduction in any medium, non-commercially, provided the new creations are licensed under identical terms as the original work and the original work is properly cited.
spellingShingle Original Paper
Ozbey, Caner
Aydemir, Isil
Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis
title Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis
title_full Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis
title_fullStr Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis
title_full_unstemmed Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis
title_short Investigation of distribution of GSK-3β signal pathway by age groups in cases of ulcerative colitis
title_sort investigation of distribution of gsk-3β signal pathway by age groups in cases of ulcerative colitis
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10257782/
https://www.ncbi.nlm.nih.gov/pubmed/37128792
http://dx.doi.org/10.47162/RJME.64.1.07
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