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Gut microbiota and type 1 diabetes: a two-sample bidirectional Mendelian randomization study

OBJECTIVE: The real causal relationship between human gut microbiota and T1D remains unclear and difficult to establish. Herein, we adopted a two-sample bidirectional mendelian randomization (MR) study to evaluate the causality between gut microbiota and T1D. METHODS: We leveraged publicly available...

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Autores principales: Luo, Manjun, Sun, Mengting, Wang, Tingting, Zhang, Senmao, Song, Xinli, Liu, Xiaoying, Wei, Jianhui, Chen, Qian, Zhong, Taowei, Qin, Jiabi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258312/
https://www.ncbi.nlm.nih.gov/pubmed/37313342
http://dx.doi.org/10.3389/fcimb.2023.1163898
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author Luo, Manjun
Sun, Mengting
Wang, Tingting
Zhang, Senmao
Song, Xinli
Liu, Xiaoying
Wei, Jianhui
Chen, Qian
Zhong, Taowei
Qin, Jiabi
author_facet Luo, Manjun
Sun, Mengting
Wang, Tingting
Zhang, Senmao
Song, Xinli
Liu, Xiaoying
Wei, Jianhui
Chen, Qian
Zhong, Taowei
Qin, Jiabi
author_sort Luo, Manjun
collection PubMed
description OBJECTIVE: The real causal relationship between human gut microbiota and T1D remains unclear and difficult to establish. Herein, we adopted a two-sample bidirectional mendelian randomization (MR) study to evaluate the causality between gut microbiota and T1D. METHODS: We leveraged publicly available genome-wide association study (GWAS) summary data to perform MR analysis. The gut microbiota-related GWAS data from 18,340 individuals from the international consortium MiBioGen were used. The summary statistic data for T1D (n = 264,137) were obtained from the latest release from the FinnGen consortium as the outcome of interest. The selection of instrumental variables conformed strictly to a series of preset inclusion and exclusion criteria. MR-Egger, weighted median, inverse variance weighted (IVW), and weighted mode methods were used to assess the causal association. The Cochran’s Q test, MR-Egger intercept test, and leave-one-out analysis were conducted to identify heterogeneity and pleiotropy. RESULTS: At the phylum level, only Bacteroidetes was indicated to have causality on T1D (OR = 1.24, 95% CI = 1.01-1.53, P = 0.044) in the IVW analysis. When it comes to their subcategories, Bacteroidia class (OR = 1.28, 95% CI = 1.06-1.53, P = 0.009, P (FDR) = 0.085), Bacteroidales order (OR = 1.28, 95% CI = 1.06-1.53, P = 0.009, P (FDR) = 0.085), and Eubacterium eligens group genus (OR = 0.64, 95% CI = 0.50-0.81, P = 2.84×10(-4), P (FDR) = 0.031) were observed to have a causal relationship with T1D in the IVW analysis. No heterogeneity and pleiotropy were detected. CONCLUSIONS: The present study reports that Bacteroidetes phylum, Bacteroidia class, and Bacteroidales order causally increase T1D risk, whereas Eubacterium eligens group genus, which belongs to the Firmicutes phylum, causally decreases T1D risk. Nevertheless, future studies are warranted to dissect the underlying mechanisms of specific bacterial taxa’s role in the pathophysiology of T1D.
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spelling pubmed-102583122023-06-13 Gut microbiota and type 1 diabetes: a two-sample bidirectional Mendelian randomization study Luo, Manjun Sun, Mengting Wang, Tingting Zhang, Senmao Song, Xinli Liu, Xiaoying Wei, Jianhui Chen, Qian Zhong, Taowei Qin, Jiabi Front Cell Infect Microbiol Cellular and Infection Microbiology OBJECTIVE: The real causal relationship between human gut microbiota and T1D remains unclear and difficult to establish. Herein, we adopted a two-sample bidirectional mendelian randomization (MR) study to evaluate the causality between gut microbiota and T1D. METHODS: We leveraged publicly available genome-wide association study (GWAS) summary data to perform MR analysis. The gut microbiota-related GWAS data from 18,340 individuals from the international consortium MiBioGen were used. The summary statistic data for T1D (n = 264,137) were obtained from the latest release from the FinnGen consortium as the outcome of interest. The selection of instrumental variables conformed strictly to a series of preset inclusion and exclusion criteria. MR-Egger, weighted median, inverse variance weighted (IVW), and weighted mode methods were used to assess the causal association. The Cochran’s Q test, MR-Egger intercept test, and leave-one-out analysis were conducted to identify heterogeneity and pleiotropy. RESULTS: At the phylum level, only Bacteroidetes was indicated to have causality on T1D (OR = 1.24, 95% CI = 1.01-1.53, P = 0.044) in the IVW analysis. When it comes to their subcategories, Bacteroidia class (OR = 1.28, 95% CI = 1.06-1.53, P = 0.009, P (FDR) = 0.085), Bacteroidales order (OR = 1.28, 95% CI = 1.06-1.53, P = 0.009, P (FDR) = 0.085), and Eubacterium eligens group genus (OR = 0.64, 95% CI = 0.50-0.81, P = 2.84×10(-4), P (FDR) = 0.031) were observed to have a causal relationship with T1D in the IVW analysis. No heterogeneity and pleiotropy were detected. CONCLUSIONS: The present study reports that Bacteroidetes phylum, Bacteroidia class, and Bacteroidales order causally increase T1D risk, whereas Eubacterium eligens group genus, which belongs to the Firmicutes phylum, causally decreases T1D risk. Nevertheless, future studies are warranted to dissect the underlying mechanisms of specific bacterial taxa’s role in the pathophysiology of T1D. Frontiers Media S.A. 2023-05-29 /pmc/articles/PMC10258312/ /pubmed/37313342 http://dx.doi.org/10.3389/fcimb.2023.1163898 Text en Copyright © 2023 Luo, Sun, Wang, Zhang, Song, Liu, Wei, Chen, Zhong and Qin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Luo, Manjun
Sun, Mengting
Wang, Tingting
Zhang, Senmao
Song, Xinli
Liu, Xiaoying
Wei, Jianhui
Chen, Qian
Zhong, Taowei
Qin, Jiabi
Gut microbiota and type 1 diabetes: a two-sample bidirectional Mendelian randomization study
title Gut microbiota and type 1 diabetes: a two-sample bidirectional Mendelian randomization study
title_full Gut microbiota and type 1 diabetes: a two-sample bidirectional Mendelian randomization study
title_fullStr Gut microbiota and type 1 diabetes: a two-sample bidirectional Mendelian randomization study
title_full_unstemmed Gut microbiota and type 1 diabetes: a two-sample bidirectional Mendelian randomization study
title_short Gut microbiota and type 1 diabetes: a two-sample bidirectional Mendelian randomization study
title_sort gut microbiota and type 1 diabetes: a two-sample bidirectional mendelian randomization study
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258312/
https://www.ncbi.nlm.nih.gov/pubmed/37313342
http://dx.doi.org/10.3389/fcimb.2023.1163898
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