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Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1

Seed dormancy is a crucial developmental transition that affects the adaption and survival of plants. Arabidopsis DELAY OF GERMINATION 1 (DOG1) is known as a master regulator of seed dormancy. However, although several upstream factors of DOG1 have been reported, the exact regulation of DOG1 is not...

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Autores principales: Han, Yongtao, Georgii, Elisabeth, Priego-Cubero, Santiago, Wurm, Christoph J., Hüther, Patrick, Huber, Gregor, Koller, Robert, Becker, Claude, Durner, Jörg, Lindermayr, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258333/
https://www.ncbi.nlm.nih.gov/pubmed/37313253
http://dx.doi.org/10.3389/fpls.2023.1124899
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author Han, Yongtao
Georgii, Elisabeth
Priego-Cubero, Santiago
Wurm, Christoph J.
Hüther, Patrick
Huber, Gregor
Koller, Robert
Becker, Claude
Durner, Jörg
Lindermayr, Christian
author_facet Han, Yongtao
Georgii, Elisabeth
Priego-Cubero, Santiago
Wurm, Christoph J.
Hüther, Patrick
Huber, Gregor
Koller, Robert
Becker, Claude
Durner, Jörg
Lindermayr, Christian
author_sort Han, Yongtao
collection PubMed
description Seed dormancy is a crucial developmental transition that affects the adaption and survival of plants. Arabidopsis DELAY OF GERMINATION 1 (DOG1) is known as a master regulator of seed dormancy. However, although several upstream factors of DOG1 have been reported, the exact regulation of DOG1 is not fully understood. Histone acetylation is an important regulatory layer, controlled by histone acetyltransferases and histone deacetylases. Histone acetylation strongly correlates with transcriptionally active chromatin, whereas heterochromatin is generally characterized by hypoacetylated histones. Here we describe that loss of function of two plant-specific histone deacetylases, HD2A and HD2B, resulted in enhanced seed dormancy in Arabidopsis. Interestingly, the silencing of HD2A and HD2B caused hyperacetylation of the DOG1 locus and promoted the expression of DOG1 during seed maturation and imbibition. Knockout of DOG1 could rescue the seed dormancy and partly rescue the disturbed development phenotype of hd2ahd2b. Transcriptomic analysis of the hd2ahd2b line shows that many genes involved in seed development were impaired. Moreover, we demonstrated that HSI2 and HSL1 interact with HD2A and HD2B. In sum, these results suggest that HSI2 and HSL1 might recruit HD2A and HD2B to DOG1 to negatively regulate DOG1 expression and to reduce seed dormancy, consequently, affecting seed development during seed maturation and promoting seed germination during imbibition.
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spelling pubmed-102583332023-06-13 Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1 Han, Yongtao Georgii, Elisabeth Priego-Cubero, Santiago Wurm, Christoph J. Hüther, Patrick Huber, Gregor Koller, Robert Becker, Claude Durner, Jörg Lindermayr, Christian Front Plant Sci Plant Science Seed dormancy is a crucial developmental transition that affects the adaption and survival of plants. Arabidopsis DELAY OF GERMINATION 1 (DOG1) is known as a master regulator of seed dormancy. However, although several upstream factors of DOG1 have been reported, the exact regulation of DOG1 is not fully understood. Histone acetylation is an important regulatory layer, controlled by histone acetyltransferases and histone deacetylases. Histone acetylation strongly correlates with transcriptionally active chromatin, whereas heterochromatin is generally characterized by hypoacetylated histones. Here we describe that loss of function of two plant-specific histone deacetylases, HD2A and HD2B, resulted in enhanced seed dormancy in Arabidopsis. Interestingly, the silencing of HD2A and HD2B caused hyperacetylation of the DOG1 locus and promoted the expression of DOG1 during seed maturation and imbibition. Knockout of DOG1 could rescue the seed dormancy and partly rescue the disturbed development phenotype of hd2ahd2b. Transcriptomic analysis of the hd2ahd2b line shows that many genes involved in seed development were impaired. Moreover, we demonstrated that HSI2 and HSL1 interact with HD2A and HD2B. In sum, these results suggest that HSI2 and HSL1 might recruit HD2A and HD2B to DOG1 to negatively regulate DOG1 expression and to reduce seed dormancy, consequently, affecting seed development during seed maturation and promoting seed germination during imbibition. Frontiers Media S.A. 2023-05-29 /pmc/articles/PMC10258333/ /pubmed/37313253 http://dx.doi.org/10.3389/fpls.2023.1124899 Text en Copyright © 2023 Han, Georgii, Priego-Cubero, Wurm, Hüther, Huber, Koller, Becker, Durner and Lindermayr https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Han, Yongtao
Georgii, Elisabeth
Priego-Cubero, Santiago
Wurm, Christoph J.
Hüther, Patrick
Huber, Gregor
Koller, Robert
Becker, Claude
Durner, Jörg
Lindermayr, Christian
Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1
title Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1
title_full Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1
title_fullStr Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1
title_full_unstemmed Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1
title_short Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1
title_sort arabidopsis histone deacetylase hd2a and hd2b regulate seed dormancy by repressing delay of germination 1
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258333/
https://www.ncbi.nlm.nih.gov/pubmed/37313253
http://dx.doi.org/10.3389/fpls.2023.1124899
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