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MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy

BACKGROUND: Mammalian infertile-20-like kinase 4 (MST4) plays major roles in the progression of malignant tumor types, but its function in gastric cancer (GC) remains poorly understood. OBJECTIVE: To investigate the regulatory mechanism of MST4 in GC. METHODS: Immunohistochemistry was used to detect...

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Detalles Bibliográficos
Autores principales: Liu, Pengwei, Li, Lin, Wang, Wei, He, Chiyi, Xu, Chunfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258413/
https://www.ncbi.nlm.nih.gov/pubmed/37313160
http://dx.doi.org/10.1016/j.heliyon.2023.e16735
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author Liu, Pengwei
Li, Lin
Wang, Wei
He, Chiyi
Xu, Chunfang
author_facet Liu, Pengwei
Li, Lin
Wang, Wei
He, Chiyi
Xu, Chunfang
author_sort Liu, Pengwei
collection PubMed
description BACKGROUND: Mammalian infertile-20-like kinase 4 (MST4) plays major roles in the progression of malignant tumor types, but its function in gastric cancer (GC) remains poorly understood. OBJECTIVE: To investigate the regulatory mechanism of MST4 in GC. METHODS: Immunohistochemistry was used to detect MST4 protein in GC tissue. Additionally, the correlation between MST4 expression and the clinicopathological characteristics and prognosis of GC was evaluated. The MST4 expression level in GC cells was measured by western blotting and quantitative real-time polymerase chain reaction. Moreover, the regulatory mechanism of MST4 was investigated in vitro and in vivo. RESULTS: Overexpression of MST4 was found in GC tissue and cell lines, which correlated to the tumor size, histological type, invasion depth, ulcer, lymph node metastasis, lymphovascular invasion, perineural invasion and TNM stage (all P < 0.01). In terms of MST4 functions in vitro, its upregulation facilitated the proliferation, migration, and invasion of GC cells. Furthermore, MST4 promoted these processes by facilitating autophagy, whereas downregulation of MST4 significantly attenuated these processes. Downregulation of MST4 also attenuated tumor growth in vivo. CONCLUSION: High expression of MST4 indicates a poor prognosis and promotes GC cell proliferation, invasion, and metastasis by enhancing autophagy.
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spelling pubmed-102584132023-06-13 MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy Liu, Pengwei Li, Lin Wang, Wei He, Chiyi Xu, Chunfang Heliyon Research Article BACKGROUND: Mammalian infertile-20-like kinase 4 (MST4) plays major roles in the progression of malignant tumor types, but its function in gastric cancer (GC) remains poorly understood. OBJECTIVE: To investigate the regulatory mechanism of MST4 in GC. METHODS: Immunohistochemistry was used to detect MST4 protein in GC tissue. Additionally, the correlation between MST4 expression and the clinicopathological characteristics and prognosis of GC was evaluated. The MST4 expression level in GC cells was measured by western blotting and quantitative real-time polymerase chain reaction. Moreover, the regulatory mechanism of MST4 was investigated in vitro and in vivo. RESULTS: Overexpression of MST4 was found in GC tissue and cell lines, which correlated to the tumor size, histological type, invasion depth, ulcer, lymph node metastasis, lymphovascular invasion, perineural invasion and TNM stage (all P < 0.01). In terms of MST4 functions in vitro, its upregulation facilitated the proliferation, migration, and invasion of GC cells. Furthermore, MST4 promoted these processes by facilitating autophagy, whereas downregulation of MST4 significantly attenuated these processes. Downregulation of MST4 also attenuated tumor growth in vivo. CONCLUSION: High expression of MST4 indicates a poor prognosis and promotes GC cell proliferation, invasion, and metastasis by enhancing autophagy. Elsevier 2023-05-29 /pmc/articles/PMC10258413/ /pubmed/37313160 http://dx.doi.org/10.1016/j.heliyon.2023.e16735 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Liu, Pengwei
Li, Lin
Wang, Wei
He, Chiyi
Xu, Chunfang
MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy
title MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy
title_full MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy
title_fullStr MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy
title_full_unstemmed MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy
title_short MST4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy
title_sort mst4 promotes proliferation, invasion, and metastasis of gastric cancer by enhancing autophagy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258413/
https://www.ncbi.nlm.nih.gov/pubmed/37313160
http://dx.doi.org/10.1016/j.heliyon.2023.e16735
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