Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro

Bacterial co-infection is one of the most common complications of SARS CoV-2 infection. Here, we present a protocol for the in vitro study of co-infection between SARS CoV-2 and Staphylococcus aureus. We describe steps for quantifying viral and bacterial replication kinetics in the same sample, with...

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Detalles Bibliográficos
Autores principales: Goncheva, Mariya I., Heinrichs, David E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258577/
https://www.ncbi.nlm.nih.gov/pubmed/37393614
http://dx.doi.org/10.1016/j.xpro.2023.102411
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author Goncheva, Mariya I.
Heinrichs, David E.
author_facet Goncheva, Mariya I.
Heinrichs, David E.
author_sort Goncheva, Mariya I.
collection PubMed
description Bacterial co-infection is one of the most common complications of SARS CoV-2 infection. Here, we present a protocol for the in vitro study of co-infection between SARS CoV-2 and Staphylococcus aureus. We describe steps for quantifying viral and bacterial replication kinetics in the same sample, with the optional extraction of host RNA and proteins. This protocol is applicable to many viral and bacterial strains and can be performed in different cell types. For complete details on the use and execution of this protocol, please refer to Goncheva et al.(1)
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spelling pubmed-102585772023-06-12 Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro Goncheva, Mariya I. Heinrichs, David E. STAR Protoc Protocol Bacterial co-infection is one of the most common complications of SARS CoV-2 infection. Here, we present a protocol for the in vitro study of co-infection between SARS CoV-2 and Staphylococcus aureus. We describe steps for quantifying viral and bacterial replication kinetics in the same sample, with the optional extraction of host RNA and proteins. This protocol is applicable to many viral and bacterial strains and can be performed in different cell types. For complete details on the use and execution of this protocol, please refer to Goncheva et al.(1) Elsevier 2023-06-12 /pmc/articles/PMC10258577/ /pubmed/37393614 http://dx.doi.org/10.1016/j.xpro.2023.102411 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Goncheva, Mariya I.
Heinrichs, David E.
Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro
title Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro
title_full Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro
title_fullStr Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro
title_full_unstemmed Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro
title_short Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro
title_sort protocol for studying co-infection between sars-cov-2 and staphylococcus aureus in vitro
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258577/
https://www.ncbi.nlm.nih.gov/pubmed/37393614
http://dx.doi.org/10.1016/j.xpro.2023.102411
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