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Treatment-naïve pemphigus patients showing IgG tissue deposits have serum IgG antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience

INTRODUCTION: Pemphigus is a heterogeneous group of autoimmune acantholytic diseases. AIM: To check whether there is a relationship between detecting IgG deposits in the direct immunofluorescence (DIF) and finding IgG antibodies against particular desmoglein (DSG) isoforms in ELISA techniques in pat...

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Autores principales: Jałowska, Magdalena, Dmochowski, Marian, Gornowicz-Porowska, Justyna, Bowszyc-Dmochowska, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258692/
https://www.ncbi.nlm.nih.gov/pubmed/37312921
http://dx.doi.org/10.5114/ada.2023.127644
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author Jałowska, Magdalena
Dmochowski, Marian
Gornowicz-Porowska, Justyna
Bowszyc-Dmochowska, Monika
author_facet Jałowska, Magdalena
Dmochowski, Marian
Gornowicz-Porowska, Justyna
Bowszyc-Dmochowska, Monika
author_sort Jałowska, Magdalena
collection PubMed
description INTRODUCTION: Pemphigus is a heterogeneous group of autoimmune acantholytic diseases. AIM: To check whether there is a relationship between detecting IgG deposits in the direct immunofluorescence (DIF) and finding IgG antibodies against particular desmoglein (DSG) isoforms in ELISA techniques in patients with pemphigus. MATERIAL AND METHODS: Single-step DIF for revealing the deposits of IgA, IgM, IgG, IgG1, IgG4 and C3, and monoanalyte ELISAs or the multiplex ELISA were used for diagnosis. The Z test for two independent proportions was used for the statistical analysis. RESULTS: We evaluated 19 consecutive treatment-naïve pemphigus patients, who exhibited IgG deposits, accompanied by other types of immunoreactants in various combinations, in DIF. Serum IgG antibodies against DSG1 were detected in 18 patients, whereas serum IgG antibodies against DSG3 were found in 10 patients. The statistical analysis showed that the proportion of anti-DSG1 antibody-positive individuals (18 of 19, 94.74%) was statistically significantly higher than the proportion of anti-DSG3 antibody-positive ones (10 of 19, 52.63%) (p = 0.0099). CONCLUSIONS: IgG deposition in the pemphigus pattern seems to be related to the presence of serum IgG antibodies against DSG1 rather than against DSG3. DSG1 may bind IgG more efficiently than DSG3 since DSG1 has a longer cytoplasmic region compared to that of DSG3.
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spelling pubmed-102586922023-06-13 Treatment-naïve pemphigus patients showing IgG tissue deposits have serum IgG antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience Jałowska, Magdalena Dmochowski, Marian Gornowicz-Porowska, Justyna Bowszyc-Dmochowska, Monika Postepy Dermatol Alergol Original Paper INTRODUCTION: Pemphigus is a heterogeneous group of autoimmune acantholytic diseases. AIM: To check whether there is a relationship between detecting IgG deposits in the direct immunofluorescence (DIF) and finding IgG antibodies against particular desmoglein (DSG) isoforms in ELISA techniques in patients with pemphigus. MATERIAL AND METHODS: Single-step DIF for revealing the deposits of IgA, IgM, IgG, IgG1, IgG4 and C3, and monoanalyte ELISAs or the multiplex ELISA were used for diagnosis. The Z test for two independent proportions was used for the statistical analysis. RESULTS: We evaluated 19 consecutive treatment-naïve pemphigus patients, who exhibited IgG deposits, accompanied by other types of immunoreactants in various combinations, in DIF. Serum IgG antibodies against DSG1 were detected in 18 patients, whereas serum IgG antibodies against DSG3 were found in 10 patients. The statistical analysis showed that the proportion of anti-DSG1 antibody-positive individuals (18 of 19, 94.74%) was statistically significantly higher than the proportion of anti-DSG3 antibody-positive ones (10 of 19, 52.63%) (p = 0.0099). CONCLUSIONS: IgG deposition in the pemphigus pattern seems to be related to the presence of serum IgG antibodies against DSG1 rather than against DSG3. DSG1 may bind IgG more efficiently than DSG3 since DSG1 has a longer cytoplasmic region compared to that of DSG3. Termedia Publishing House 2023-06-01 2023-04 /pmc/articles/PMC10258692/ /pubmed/37312921 http://dx.doi.org/10.5114/ada.2023.127644 Text en Copyright: © 2023 Termedia Sp. z o. o. https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Original Paper
Jałowska, Magdalena
Dmochowski, Marian
Gornowicz-Porowska, Justyna
Bowszyc-Dmochowska, Monika
Treatment-naïve pemphigus patients showing IgG tissue deposits have serum IgG antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience
title Treatment-naïve pemphigus patients showing IgG tissue deposits have serum IgG antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience
title_full Treatment-naïve pemphigus patients showing IgG tissue deposits have serum IgG antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience
title_fullStr Treatment-naïve pemphigus patients showing IgG tissue deposits have serum IgG antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience
title_full_unstemmed Treatment-naïve pemphigus patients showing IgG tissue deposits have serum IgG antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience
title_short Treatment-naïve pemphigus patients showing IgG tissue deposits have serum IgG antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience
title_sort treatment-naïve pemphigus patients showing igg tissue deposits have serum igg antibodies against desmoglein 1 detected more often than those against desmoglein 3: a monocentre laboratory experience
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258692/
https://www.ncbi.nlm.nih.gov/pubmed/37312921
http://dx.doi.org/10.5114/ada.2023.127644
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