Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex

[Image: see text] We observed the mid-infrared (MIR) response of a single pigment of bacteriochlorophyll a at the B800 binding site of a light-harvesting 2 complex. At a temperature of 1.5 K, a single complex in a spatially isolated spot in a near-infrared (NIR) fluorescence image was selected and w...

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Autores principales: Otomo, Kohei, Dewa, Takehisa, Matsushita, Michio, Fujiyoshi, Satoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258799/
https://www.ncbi.nlm.nih.gov/pubmed/37222077
http://dx.doi.org/10.1021/acs.jpcb.3c00284
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author Otomo, Kohei
Dewa, Takehisa
Matsushita, Michio
Fujiyoshi, Satoru
author_facet Otomo, Kohei
Dewa, Takehisa
Matsushita, Michio
Fujiyoshi, Satoru
author_sort Otomo, Kohei
collection PubMed
description [Image: see text] We observed the mid-infrared (MIR) response of a single pigment of bacteriochlorophyll a at the B800 binding site of a light-harvesting 2 complex. At a temperature of 1.5 K, a single complex in a spatially isolated spot in a near-infrared (NIR) fluorescence image was selected and was simultaneously irradiated with MIR and NIR light. We found that the temporal behavior of the NIR fluorescence excitation spectrum of individual pigments in a single complex was modulated by the MIR irradiation at 1650 cm(–1). The MIR modulation of a single pigment was linearly proportional to the MIR intensity. The MIR linear response was detected in the range from 1580 to 1670 cm(–1).
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spelling pubmed-102587992023-06-13 Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex Otomo, Kohei Dewa, Takehisa Matsushita, Michio Fujiyoshi, Satoru J Phys Chem B [Image: see text] We observed the mid-infrared (MIR) response of a single pigment of bacteriochlorophyll a at the B800 binding site of a light-harvesting 2 complex. At a temperature of 1.5 K, a single complex in a spatially isolated spot in a near-infrared (NIR) fluorescence image was selected and was simultaneously irradiated with MIR and NIR light. We found that the temporal behavior of the NIR fluorescence excitation spectrum of individual pigments in a single complex was modulated by the MIR irradiation at 1650 cm(–1). The MIR modulation of a single pigment was linearly proportional to the MIR intensity. The MIR linear response was detected in the range from 1580 to 1670 cm(–1). American Chemical Society 2023-05-24 /pmc/articles/PMC10258799/ /pubmed/37222077 http://dx.doi.org/10.1021/acs.jpcb.3c00284 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Otomo, Kohei
Dewa, Takehisa
Matsushita, Michio
Fujiyoshi, Satoru
Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex
title Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex
title_full Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex
title_fullStr Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex
title_full_unstemmed Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex
title_short Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex
title_sort cryogenic single-molecule fluorescence detection of the mid-infrared response of an intrinsic pigment in a light-harvesting complex
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258799/
https://www.ncbi.nlm.nih.gov/pubmed/37222077
http://dx.doi.org/10.1021/acs.jpcb.3c00284
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