Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668

Bifidobacteria are representative intestinal probiotics that have extremely high application value in the food and medical fields. However, the lack of molecular biology tools limits the research on functional genes and mechanisms of bifidobacteria. The application of an accurate and efficient CRISP...

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Autores principales: Li, Jiao, Song, Xin, Xiong, Zhiqiang, Wang, Guangqiang, Xia, Yongjun, Yang, Yijin, Ai, Lianzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10259016/
https://www.ncbi.nlm.nih.gov/pubmed/37308875
http://dx.doi.org/10.1186/s12934-023-02094-2
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author Li, Jiao
Song, Xin
Xiong, Zhiqiang
Wang, Guangqiang
Xia, Yongjun
Yang, Yijin
Ai, Lianzhong
author_facet Li, Jiao
Song, Xin
Xiong, Zhiqiang
Wang, Guangqiang
Xia, Yongjun
Yang, Yijin
Ai, Lianzhong
author_sort Li, Jiao
collection PubMed
description Bifidobacteria are representative intestinal probiotics that have extremely high application value in the food and medical fields. However, the lack of molecular biology tools limits the research on functional genes and mechanisms of bifidobacteria. The application of an accurate and efficient CRISPR system to genome engineering can fill the gap in efficient genetic tools for bifidobacteria. In this study, CRISPR system of B. animalis AR668 was established, which successfully knocked out gene 0348 and gene 0208. The influence of different homology arms and fragments on the knockout effect of the system was explored. In addition, the inducible plasmid curing system of bifidobacteria was innovatively established. This study contributes to the genetic modification and functional mechanism analysis of bifidobacteria.
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spelling pubmed-102590162023-06-13 Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668 Li, Jiao Song, Xin Xiong, Zhiqiang Wang, Guangqiang Xia, Yongjun Yang, Yijin Ai, Lianzhong Microb Cell Fact Research Bifidobacteria are representative intestinal probiotics that have extremely high application value in the food and medical fields. However, the lack of molecular biology tools limits the research on functional genes and mechanisms of bifidobacteria. The application of an accurate and efficient CRISPR system to genome engineering can fill the gap in efficient genetic tools for bifidobacteria. In this study, CRISPR system of B. animalis AR668 was established, which successfully knocked out gene 0348 and gene 0208. The influence of different homology arms and fragments on the knockout effect of the system was explored. In addition, the inducible plasmid curing system of bifidobacteria was innovatively established. This study contributes to the genetic modification and functional mechanism analysis of bifidobacteria. BioMed Central 2023-06-12 /pmc/articles/PMC10259016/ /pubmed/37308875 http://dx.doi.org/10.1186/s12934-023-02094-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Jiao
Song, Xin
Xiong, Zhiqiang
Wang, Guangqiang
Xia, Yongjun
Yang, Yijin
Ai, Lianzhong
Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
title Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
title_full Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
title_fullStr Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
title_full_unstemmed Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
title_short Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
title_sort establishment of crispr-cas9 system in bifidobacteria animalis ar668
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10259016/
https://www.ncbi.nlm.nih.gov/pubmed/37308875
http://dx.doi.org/10.1186/s12934-023-02094-2
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