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Human Transplant Kidneys on Normothermic Machine Perfusion Display Endocrine Activity
Normothermic machine perfusion (NMP) is an alternative to hypothermic machine perfusion (HMP) for donor kidney preservation before transplantation. Contrary to HMP, NMP allows for functional assessment of donor kidneys because normothermic conditions allow for metabolic activity. The kidneys are key...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10259641/ https://www.ncbi.nlm.nih.gov/pubmed/37313312 http://dx.doi.org/10.1097/TXD.0000000000001503 |
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author | Lin, Hui Du, Zhaoyu Bouari, Sarah Rijkse, Elsaline Cristoferi, Iacopo Obser, Anja Czogalla, Jan Danser, A.H. Jan Minnee, Robert C. Hoogduijn, Martin J. |
author_facet | Lin, Hui Du, Zhaoyu Bouari, Sarah Rijkse, Elsaline Cristoferi, Iacopo Obser, Anja Czogalla, Jan Danser, A.H. Jan Minnee, Robert C. Hoogduijn, Martin J. |
author_sort | Lin, Hui |
collection | PubMed |
description | Normothermic machine perfusion (NMP) is an alternative to hypothermic machine perfusion (HMP) for donor kidney preservation before transplantation. Contrary to HMP, NMP allows for functional assessment of donor kidneys because normothermic conditions allow for metabolic activity. The kidneys are key producers of hormones. Yet, it remains unknown whether donor kidneys during NMP display endocrine functions. METHODS. Fifteen donor kidneys were subjected to HMP followed by 2 h of NMP before transplantation. NMP perfusate was collected at 3 time points (0, 1, 2 h) for the measurements of prorenin/renin, erythropoietin (EPO), and vitamin D, and urine samples were collected at 1 h and 2 h for urodilatin measurement. Fifteen HMP perfusate samples were collected for the same measurements. RESULTS. Kidneys on NMP secreted significantly more prorenin, renin, EPO, and active vitamin D than during HMP. EPO and vitamin D secretion remained stable during 2 h of NMP, whereas the prorenin release rate increased and renin release rate decreased after 1 h. Donation after brain death kidneys secreted more vitamin D and less EPO during NMP than donation after circulatory death kidneys. Twelve donor kidneys produced urine during NMP and released detectable levels of urodilatin. Kidneys exhibited a large variation in hormone release rates. No significant differences were found in hormone release capacity between delayed graft function (DGF) and non-DGF kidneys, and no significant correlations were found between hormone release rates and the duration of DGF or 1-mo posttransplant serum creatinine levels. CONCLUSIONS. Human transplant kidneys display endocrine activity during NMP. To explore whether correlations exist between hormone release rates and posttransplant kidney function, large numbers of kidneys are required. |
format | Online Article Text |
id | pubmed-10259641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-102596412023-06-13 Human Transplant Kidneys on Normothermic Machine Perfusion Display Endocrine Activity Lin, Hui Du, Zhaoyu Bouari, Sarah Rijkse, Elsaline Cristoferi, Iacopo Obser, Anja Czogalla, Jan Danser, A.H. Jan Minnee, Robert C. Hoogduijn, Martin J. Transplant Direct Kidney Transplantation Normothermic machine perfusion (NMP) is an alternative to hypothermic machine perfusion (HMP) for donor kidney preservation before transplantation. Contrary to HMP, NMP allows for functional assessment of donor kidneys because normothermic conditions allow for metabolic activity. The kidneys are key producers of hormones. Yet, it remains unknown whether donor kidneys during NMP display endocrine functions. METHODS. Fifteen donor kidneys were subjected to HMP followed by 2 h of NMP before transplantation. NMP perfusate was collected at 3 time points (0, 1, 2 h) for the measurements of prorenin/renin, erythropoietin (EPO), and vitamin D, and urine samples were collected at 1 h and 2 h for urodilatin measurement. Fifteen HMP perfusate samples were collected for the same measurements. RESULTS. Kidneys on NMP secreted significantly more prorenin, renin, EPO, and active vitamin D than during HMP. EPO and vitamin D secretion remained stable during 2 h of NMP, whereas the prorenin release rate increased and renin release rate decreased after 1 h. Donation after brain death kidneys secreted more vitamin D and less EPO during NMP than donation after circulatory death kidneys. Twelve donor kidneys produced urine during NMP and released detectable levels of urodilatin. Kidneys exhibited a large variation in hormone release rates. No significant differences were found in hormone release capacity between delayed graft function (DGF) and non-DGF kidneys, and no significant correlations were found between hormone release rates and the duration of DGF or 1-mo posttransplant serum creatinine levels. CONCLUSIONS. Human transplant kidneys display endocrine activity during NMP. To explore whether correlations exist between hormone release rates and posttransplant kidney function, large numbers of kidneys are required. Lippincott Williams & Wilkins 2023-06-09 /pmc/articles/PMC10259641/ /pubmed/37313312 http://dx.doi.org/10.1097/TXD.0000000000001503 Text en Copyright © 2023 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Kidney Transplantation Lin, Hui Du, Zhaoyu Bouari, Sarah Rijkse, Elsaline Cristoferi, Iacopo Obser, Anja Czogalla, Jan Danser, A.H. Jan Minnee, Robert C. Hoogduijn, Martin J. Human Transplant Kidneys on Normothermic Machine Perfusion Display Endocrine Activity |
title | Human Transplant Kidneys on Normothermic Machine Perfusion Display Endocrine Activity |
title_full | Human Transplant Kidneys on Normothermic Machine Perfusion Display Endocrine Activity |
title_fullStr | Human Transplant Kidneys on Normothermic Machine Perfusion Display Endocrine Activity |
title_full_unstemmed | Human Transplant Kidneys on Normothermic Machine Perfusion Display Endocrine Activity |
title_short | Human Transplant Kidneys on Normothermic Machine Perfusion Display Endocrine Activity |
title_sort | human transplant kidneys on normothermic machine perfusion display endocrine activity |
topic | Kidney Transplantation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10259641/ https://www.ncbi.nlm.nih.gov/pubmed/37313312 http://dx.doi.org/10.1097/TXD.0000000000001503 |
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