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MDB-30. IDENTIFICATION AND CHARACTERIZATION OF NOVEL TRANSCRIBED ULTRACONSERVED REGIONS AS LONG NON-CODING RNAS IN MEDULLOBLASTOMA

Medulloblastoma is the most common pediatric brain tumor. While the 5-year survival rate for medulloblastoma patients has improved with molecular subgrouping, children who undergo standard of care treatments frequently experience permanent neurological deficits. Additionally, under-characterized med...

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Autores principales: Hudson, Kadie, Gibert Jr., Myron K, Zhang, Ying, Dube, Collin, Saha, Shekhar, Vernon, Ashley, Abounader, Roger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10260161/
http://dx.doi.org/10.1093/neuonc/noad073.262
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author Hudson, Kadie
Gibert Jr., Myron K
Zhang, Ying
Dube, Collin
Saha, Shekhar
Vernon, Ashley
Abounader, Roger
author_facet Hudson, Kadie
Gibert Jr., Myron K
Zhang, Ying
Dube, Collin
Saha, Shekhar
Vernon, Ashley
Abounader, Roger
author_sort Hudson, Kadie
collection PubMed
description Medulloblastoma is the most common pediatric brain tumor. While the 5-year survival rate for medulloblastoma patients has improved with molecular subgrouping, children who undergo standard of care treatments frequently experience permanent neurological deficits. Additionally, under-characterized medulloblastoma subgroups lack targeted therapies, correlating to lower survival rates for these patients. To find new therapies, a better understanding of the molecular basis of medulloblastoma is needed. This work investigates the role of “Transcribed Ultra-Conserved Regions” (TUCRs) in medulloblastoma about which there is no published literature. TUCRs represent a distinct group of 481 putative lncRNAs that are 100% conserved across human, mouse, and rat genomes. These ultra-conserved elements change 20 times slower than the average genomic region and are therefore under intense negative selection within multiple species. Since their discovery, TUCRs have only been correlated to a handful of cancers and remain exceedingly underexamined within cancer research. Using RNA-sequencing and differential gene expression analysis of medulloblastoma tumors as compared to normal human cerebellum samples, we identified a multitude of differentially expressed TUCRs spanning across all TUCR genomic annotations. In vitro experimentation using several medulloblastoma established cell lines indicated significant upregulation of these TUCR transcripts. We are currently investigating the functional effects and mechanisms of action of select differentially expressed TUCRs in medulloblastoma. Altogether, our work is investigating the oncogenic and tumor suppressive nature of TUCRs. It represents the first study of TUCRs in medulloblastoma that will significantly advance our basic understanding of this highly understudied class of molecules.
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spelling pubmed-102601612023-06-13 MDB-30. IDENTIFICATION AND CHARACTERIZATION OF NOVEL TRANSCRIBED ULTRACONSERVED REGIONS AS LONG NON-CODING RNAS IN MEDULLOBLASTOMA Hudson, Kadie Gibert Jr., Myron K Zhang, Ying Dube, Collin Saha, Shekhar Vernon, Ashley Abounader, Roger Neuro Oncol Final Category: Medulloblastomas - MDB Medulloblastoma is the most common pediatric brain tumor. While the 5-year survival rate for medulloblastoma patients has improved with molecular subgrouping, children who undergo standard of care treatments frequently experience permanent neurological deficits. Additionally, under-characterized medulloblastoma subgroups lack targeted therapies, correlating to lower survival rates for these patients. To find new therapies, a better understanding of the molecular basis of medulloblastoma is needed. This work investigates the role of “Transcribed Ultra-Conserved Regions” (TUCRs) in medulloblastoma about which there is no published literature. TUCRs represent a distinct group of 481 putative lncRNAs that are 100% conserved across human, mouse, and rat genomes. These ultra-conserved elements change 20 times slower than the average genomic region and are therefore under intense negative selection within multiple species. Since their discovery, TUCRs have only been correlated to a handful of cancers and remain exceedingly underexamined within cancer research. Using RNA-sequencing and differential gene expression analysis of medulloblastoma tumors as compared to normal human cerebellum samples, we identified a multitude of differentially expressed TUCRs spanning across all TUCR genomic annotations. In vitro experimentation using several medulloblastoma established cell lines indicated significant upregulation of these TUCR transcripts. We are currently investigating the functional effects and mechanisms of action of select differentially expressed TUCRs in medulloblastoma. Altogether, our work is investigating the oncogenic and tumor suppressive nature of TUCRs. It represents the first study of TUCRs in medulloblastoma that will significantly advance our basic understanding of this highly understudied class of molecules. Oxford University Press 2023-06-12 /pmc/articles/PMC10260161/ http://dx.doi.org/10.1093/neuonc/noad073.262 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Final Category: Medulloblastomas - MDB
Hudson, Kadie
Gibert Jr., Myron K
Zhang, Ying
Dube, Collin
Saha, Shekhar
Vernon, Ashley
Abounader, Roger
MDB-30. IDENTIFICATION AND CHARACTERIZATION OF NOVEL TRANSCRIBED ULTRACONSERVED REGIONS AS LONG NON-CODING RNAS IN MEDULLOBLASTOMA
title MDB-30. IDENTIFICATION AND CHARACTERIZATION OF NOVEL TRANSCRIBED ULTRACONSERVED REGIONS AS LONG NON-CODING RNAS IN MEDULLOBLASTOMA
title_full MDB-30. IDENTIFICATION AND CHARACTERIZATION OF NOVEL TRANSCRIBED ULTRACONSERVED REGIONS AS LONG NON-CODING RNAS IN MEDULLOBLASTOMA
title_fullStr MDB-30. IDENTIFICATION AND CHARACTERIZATION OF NOVEL TRANSCRIBED ULTRACONSERVED REGIONS AS LONG NON-CODING RNAS IN MEDULLOBLASTOMA
title_full_unstemmed MDB-30. IDENTIFICATION AND CHARACTERIZATION OF NOVEL TRANSCRIBED ULTRACONSERVED REGIONS AS LONG NON-CODING RNAS IN MEDULLOBLASTOMA
title_short MDB-30. IDENTIFICATION AND CHARACTERIZATION OF NOVEL TRANSCRIBED ULTRACONSERVED REGIONS AS LONG NON-CODING RNAS IN MEDULLOBLASTOMA
title_sort mdb-30. identification and characterization of novel transcribed ultraconserved regions as long non-coding rnas in medulloblastoma
topic Final Category: Medulloblastomas - MDB
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10260161/
http://dx.doi.org/10.1093/neuonc/noad073.262
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