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Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint
Dielectrophoresis is an electric field-based technique for moving neutral particles through a fluid. When used for particle separation, dielectrophoresis has many advantages compared to other methods, like providing label-free operation with greater control of the separation forces. In this paper, w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10260938/ https://www.ncbi.nlm.nih.gov/pubmed/37308526 http://dx.doi.org/10.1038/s41598-023-36502-9 |
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author | Valijam, Shayan Nilsson, Daniel P. G. Malyshev, Dmitry Öberg, Rasmus Salehi, Alireza Andersson, Magnus |
author_facet | Valijam, Shayan Nilsson, Daniel P. G. Malyshev, Dmitry Öberg, Rasmus Salehi, Alireza Andersson, Magnus |
author_sort | Valijam, Shayan |
collection | PubMed |
description | Dielectrophoresis is an electric field-based technique for moving neutral particles through a fluid. When used for particle separation, dielectrophoresis has many advantages compared to other methods, like providing label-free operation with greater control of the separation forces. In this paper, we design, build, and test a low-voltage dielectrophoretic device using a 3D printing approach. This lab-on-a-chip device fits on a microscope glass slide and incorporates microfluidic channels for particle separation. First, we use multiphysics simulations to evaluate the separation efficiency of the prospective device and guide the design process. Second, we fabricate the device in PDMS (polydimethylsiloxane) by using 3D-printed moulds that contain patterns of the channels and electrodes. The imprint of the electrodes is then filled with silver conductive paint, making a 9-pole comb electrode. Lastly, we evaluate the separation efficiency of our device by introducing a mixture of 3 μm and 10 μm polystyrene particles and tracking their progression. Our device is able to efficiently separate these particles when the electrodes are energized with ±12 V at 75 kHz. Overall, our method allows the fabrication of cheap and effective dielectrophoretic microfluidic devices using commercial off-the-shelf equipment. |
format | Online Article Text |
id | pubmed-10260938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-102609382023-06-15 Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint Valijam, Shayan Nilsson, Daniel P. G. Malyshev, Dmitry Öberg, Rasmus Salehi, Alireza Andersson, Magnus Sci Rep Article Dielectrophoresis is an electric field-based technique for moving neutral particles through a fluid. When used for particle separation, dielectrophoresis has many advantages compared to other methods, like providing label-free operation with greater control of the separation forces. In this paper, we design, build, and test a low-voltage dielectrophoretic device using a 3D printing approach. This lab-on-a-chip device fits on a microscope glass slide and incorporates microfluidic channels for particle separation. First, we use multiphysics simulations to evaluate the separation efficiency of the prospective device and guide the design process. Second, we fabricate the device in PDMS (polydimethylsiloxane) by using 3D-printed moulds that contain patterns of the channels and electrodes. The imprint of the electrodes is then filled with silver conductive paint, making a 9-pole comb electrode. Lastly, we evaluate the separation efficiency of our device by introducing a mixture of 3 μm and 10 μm polystyrene particles and tracking their progression. Our device is able to efficiently separate these particles when the electrodes are energized with ±12 V at 75 kHz. Overall, our method allows the fabrication of cheap and effective dielectrophoretic microfluidic devices using commercial off-the-shelf equipment. Nature Publishing Group UK 2023-06-12 /pmc/articles/PMC10260938/ /pubmed/37308526 http://dx.doi.org/10.1038/s41598-023-36502-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Valijam, Shayan Nilsson, Daniel P. G. Malyshev, Dmitry Öberg, Rasmus Salehi, Alireza Andersson, Magnus Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint |
title | Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint |
title_full | Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint |
title_fullStr | Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint |
title_full_unstemmed | Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint |
title_short | Fabricating a dielectrophoretic microfluidic device using 3D-printed moulds and silver conductive paint |
title_sort | fabricating a dielectrophoretic microfluidic device using 3d-printed moulds and silver conductive paint |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10260938/ https://www.ncbi.nlm.nih.gov/pubmed/37308526 http://dx.doi.org/10.1038/s41598-023-36502-9 |
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