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Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis

Heterosis contributes greatly to the worldwide agricultural yield. However, the molecular mechanism underlying heterosis remains unclear. This study took advantage of Arabidopsis intraspecific hybrids to identify heterosis-related metabolites. Forty-six intraspecific hybrids were used to examine par...

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Autores principales: Le, Quynh Thi Ngoc, Sugi, Naoya, Yamaguchi, Masaaki, Hirayama, Touko, Kobayashi, Makoto, Suzuki, Yutaka, Kusano, Miyako, Shiba, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10261038/
https://www.ncbi.nlm.nih.gov/pubmed/37308530
http://dx.doi.org/10.1038/s41598-023-36618-y
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author Le, Quynh Thi Ngoc
Sugi, Naoya
Yamaguchi, Masaaki
Hirayama, Touko
Kobayashi, Makoto
Suzuki, Yutaka
Kusano, Miyako
Shiba, Hiroshi
author_facet Le, Quynh Thi Ngoc
Sugi, Naoya
Yamaguchi, Masaaki
Hirayama, Touko
Kobayashi, Makoto
Suzuki, Yutaka
Kusano, Miyako
Shiba, Hiroshi
author_sort Le, Quynh Thi Ngoc
collection PubMed
description Heterosis contributes greatly to the worldwide agricultural yield. However, the molecular mechanism underlying heterosis remains unclear. This study took advantage of Arabidopsis intraspecific hybrids to identify heterosis-related metabolites. Forty-six intraspecific hybrids were used to examine parental effects on seed area and germination time. The degree of heterosis was evaluated based on biomass: combinations showing high heterosis of F(1) hybrids exhibited a biomass increase from 6.1 to 44% over the better parent value (BPV), whereas that of the low- and no-heterosis hybrids ranged from − 19.8 to 9.8% over the BPV. Metabolomics analyses of F(1) hybrids with high heterosis and those with low one suggested that changes in TCA cycle intermediates are key factors that control growth. Notably, higher fumarate/malate ratios were observed in the high heterosis F(1) hybrids, suggesting they provide metabolic support associated with the increased biomass. These hybrids may produce more energy-intensive biomass by speeding up the efficiency of TCA fluxes. However, the expression levels of TCA-process-related genes in F(1) hybrids were not associated with the intensity of heterosis, suggesting that the post-transcriptional or post-translational regulation of these genes may affect the productivity of the intermediates in the TCA cycle.
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spelling pubmed-102610382023-06-15 Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis Le, Quynh Thi Ngoc Sugi, Naoya Yamaguchi, Masaaki Hirayama, Touko Kobayashi, Makoto Suzuki, Yutaka Kusano, Miyako Shiba, Hiroshi Sci Rep Article Heterosis contributes greatly to the worldwide agricultural yield. However, the molecular mechanism underlying heterosis remains unclear. This study took advantage of Arabidopsis intraspecific hybrids to identify heterosis-related metabolites. Forty-six intraspecific hybrids were used to examine parental effects on seed area and germination time. The degree of heterosis was evaluated based on biomass: combinations showing high heterosis of F(1) hybrids exhibited a biomass increase from 6.1 to 44% over the better parent value (BPV), whereas that of the low- and no-heterosis hybrids ranged from − 19.8 to 9.8% over the BPV. Metabolomics analyses of F(1) hybrids with high heterosis and those with low one suggested that changes in TCA cycle intermediates are key factors that control growth. Notably, higher fumarate/malate ratios were observed in the high heterosis F(1) hybrids, suggesting they provide metabolic support associated with the increased biomass. These hybrids may produce more energy-intensive biomass by speeding up the efficiency of TCA fluxes. However, the expression levels of TCA-process-related genes in F(1) hybrids were not associated with the intensity of heterosis, suggesting that the post-transcriptional or post-translational regulation of these genes may affect the productivity of the intermediates in the TCA cycle. Nature Publishing Group UK 2023-06-12 /pmc/articles/PMC10261038/ /pubmed/37308530 http://dx.doi.org/10.1038/s41598-023-36618-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Le, Quynh Thi Ngoc
Sugi, Naoya
Yamaguchi, Masaaki
Hirayama, Touko
Kobayashi, Makoto
Suzuki, Yutaka
Kusano, Miyako
Shiba, Hiroshi
Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis
title Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis
title_full Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis
title_fullStr Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis
title_full_unstemmed Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis
title_short Morphological and metabolomics profiling of intraspecific Arabidopsis hybrids in relation to biomass heterosis
title_sort morphological and metabolomics profiling of intraspecific arabidopsis hybrids in relation to biomass heterosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10261038/
https://www.ncbi.nlm.nih.gov/pubmed/37308530
http://dx.doi.org/10.1038/s41598-023-36618-y
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