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Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue
Mechanosensory ion channels are proteins that are sensitive to mechanical forces. They are found in tissues throughout the body and play an important role in bone remodeling by sensing changes in mechanical stress and transmitting signals to bone-forming cells. Orthodontic tooth movement (OTM) is a...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10261143/ https://www.ncbi.nlm.nih.gov/pubmed/37308580 http://dx.doi.org/10.1038/s41598-023-36699-9 |
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author | Nottmeier, Cita Lavicky, Josef Gonzalez Lopez, Marcos Knauth, Sarah Kahl-Nieke, Bärbel Amling, Michael Schinke, Thorsten Helms, Jill Krivanek, Jan Koehne, Till Petersen, Julian |
author_facet | Nottmeier, Cita Lavicky, Josef Gonzalez Lopez, Marcos Knauth, Sarah Kahl-Nieke, Bärbel Amling, Michael Schinke, Thorsten Helms, Jill Krivanek, Jan Koehne, Till Petersen, Julian |
author_sort | Nottmeier, Cita |
collection | PubMed |
description | Mechanosensory ion channels are proteins that are sensitive to mechanical forces. They are found in tissues throughout the body and play an important role in bone remodeling by sensing changes in mechanical stress and transmitting signals to bone-forming cells. Orthodontic tooth movement (OTM) is a prime example of mechanically induced bone remodeling. However, the cell-specific role of the ion channels Piezo1 and Piezo2 in OTM has not been investigated yet. Here we first identify the expression of PIEZO1/2 in the dentoalveolar hard tissues. Results showed that PIEZO1 was expressed in odontoblasts, osteoblasts, and osteocytes, while PIEZO2 was localized in odontoblasts and cementoblasts. We therefore used a Piezo1(floxed/floxed) mouse model in combination with Dmp1(cre) to inactivate Piezo1 in mature osteoblasts/cementoblasts, osteocytes/cementocytes, and odontoblasts. Inactivation of Piezo1 in these cells did not affect the overall morphology of the skull but caused significant bone loss in the craniofacial skeleton. Histological analysis revealed a significantly increased number of osteoclasts in Piezo1(floxed/floxed);Dmp1(cre) mice, while osteoblasts were not affected. Despite this increased number of osteoclasts, orthodontic tooth movement was not altered in these mice. Our results suggest that despite Piezo1 being crucial for osteoclast function, it may be dispensable for mechanical sensing of bone remodeling. |
format | Online Article Text |
id | pubmed-10261143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-102611432023-06-15 Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue Nottmeier, Cita Lavicky, Josef Gonzalez Lopez, Marcos Knauth, Sarah Kahl-Nieke, Bärbel Amling, Michael Schinke, Thorsten Helms, Jill Krivanek, Jan Koehne, Till Petersen, Julian Sci Rep Article Mechanosensory ion channels are proteins that are sensitive to mechanical forces. They are found in tissues throughout the body and play an important role in bone remodeling by sensing changes in mechanical stress and transmitting signals to bone-forming cells. Orthodontic tooth movement (OTM) is a prime example of mechanically induced bone remodeling. However, the cell-specific role of the ion channels Piezo1 and Piezo2 in OTM has not been investigated yet. Here we first identify the expression of PIEZO1/2 in the dentoalveolar hard tissues. Results showed that PIEZO1 was expressed in odontoblasts, osteoblasts, and osteocytes, while PIEZO2 was localized in odontoblasts and cementoblasts. We therefore used a Piezo1(floxed/floxed) mouse model in combination with Dmp1(cre) to inactivate Piezo1 in mature osteoblasts/cementoblasts, osteocytes/cementocytes, and odontoblasts. Inactivation of Piezo1 in these cells did not affect the overall morphology of the skull but caused significant bone loss in the craniofacial skeleton. Histological analysis revealed a significantly increased number of osteoclasts in Piezo1(floxed/floxed);Dmp1(cre) mice, while osteoblasts were not affected. Despite this increased number of osteoclasts, orthodontic tooth movement was not altered in these mice. Our results suggest that despite Piezo1 being crucial for osteoclast function, it may be dispensable for mechanical sensing of bone remodeling. Nature Publishing Group UK 2023-06-12 /pmc/articles/PMC10261143/ /pubmed/37308580 http://dx.doi.org/10.1038/s41598-023-36699-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nottmeier, Cita Lavicky, Josef Gonzalez Lopez, Marcos Knauth, Sarah Kahl-Nieke, Bärbel Amling, Michael Schinke, Thorsten Helms, Jill Krivanek, Jan Koehne, Till Petersen, Julian Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue |
title | Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue |
title_full | Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue |
title_fullStr | Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue |
title_full_unstemmed | Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue |
title_short | Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue |
title_sort | mechanical-induced bone remodeling does not depend on piezo1 in dentoalveolar hard tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10261143/ https://www.ncbi.nlm.nih.gov/pubmed/37308580 http://dx.doi.org/10.1038/s41598-023-36699-9 |
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