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Differentiation of stem cell-derived pancreatic progenitors into insulin-secreting islet clusters in a multiwell-based static 3D culture system
Orbital shaker-based suspension culture systems have been in widespread use for differentiating human pluripotent stem cell (hPSC)-derived pancreatic progenitors toward islet-like clusters during endocrine induction stages. However, reproducibility between experiments is hampered by variable degrees...
Autores principales: | Liang, Shenghui, Zhao, Jia, Baker, Robert K., Tran, Elisa, Zhan, Lisa, Kieffer, Timothy J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10261893/ https://www.ncbi.nlm.nih.gov/pubmed/37323565 http://dx.doi.org/10.1016/j.crmeth.2023.100466 |
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