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Engineering CpG island DNA methylation in pluripotent cells through synthetic CpG-free ssDNA insertion

Cellular differentiation requires global changes to DNA methylation (DNAme), where it functions to regulate transcription factor, chromatin remodeling activity, and genome interpretation. Here, we describe a simple DNAme engineering approach in pluripotent stem cells (PSCs) that stably extends DNAme...

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Detalles Bibliográficos
Autores principales:	Tompkins, Joshua, Lizhar, Elizabeth, Shokrani, Alireza, Wu, Xiwei, Berley, Jordan, Kamali, Diba, Hussey, Deborah, Cerneckis, Jonas, Kang, Tae Hyuk, Wang, Jinhui, Tsark, Walter, Zeng, Defu, Godatha, Swetha, Natarajan, Rama, Riggs, Arthur
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10261899/ https://www.ncbi.nlm.nih.gov/pubmed/37323577 http://dx.doi.org/10.1016/j.crmeth.2023.100465

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