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Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients

HIV-associated lipodystrophy (HIVLD) is a metabolic condition with an irregularity in the production of lipoprotein particles, and its occurrence varies among HIV-infected patients. MTP and ABCG2 genes have a role in the transport of lipoproteins. The polymorphisms of MTP -493G/T and ABCG2 34G/A aff...

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Autores principales: Singh, HariOm, Jori, Chandrashekhar, Shyamveer, Mahajan, Supriya D., Aalinkeel, Ravikumar, Kaliyappan, Kathiravan, Schwartz, Stanley A., Bhattacharya, Meenakshi, Shaikh, Ruhi, Salve, Madhukar, Deshmukh, Jyoti, Ali, Nemat, Parvez, Mohammad Khalid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262045/
https://www.ncbi.nlm.nih.gov/pubmed/37324630
http://dx.doi.org/10.3389/fcvm.2023.1177054
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author Singh, HariOm
Jori, Chandrashekhar
Shyamveer
Mahajan, Supriya D.
Aalinkeel, Ravikumar
Kaliyappan, Kathiravan
Schwartz, Stanley A.
Bhattacharya, Meenakshi
Shaikh, Ruhi
Salve, Madhukar
Deshmukh, Jyoti
Ali, Nemat
Parvez, Mohammad Khalid
author_facet Singh, HariOm
Jori, Chandrashekhar
Shyamveer
Mahajan, Supriya D.
Aalinkeel, Ravikumar
Kaliyappan, Kathiravan
Schwartz, Stanley A.
Bhattacharya, Meenakshi
Shaikh, Ruhi
Salve, Madhukar
Deshmukh, Jyoti
Ali, Nemat
Parvez, Mohammad Khalid
author_sort Singh, HariOm
collection PubMed
description HIV-associated lipodystrophy (HIVLD) is a metabolic condition with an irregularity in the production of lipoprotein particles, and its occurrence varies among HIV-infected patients. MTP and ABCG2 genes have a role in the transport of lipoproteins. The polymorphisms of MTP -493G/T and ABCG2 34G/A affect its expression and influence the secretion and transportation of lipoproteins. Hence, we investigated the MTP -493G/T and ABCG2 34G/A polymorphisms in 187 HIV-infected patients (64 with HIVLD and 123 without HIVLD) along with 139 healthy controls using polymerase chain reaction (PCR)-restriction fragment length polymorphism and expression analysis using real-time PCR. ABCG2 34A allele showed an insignificantly reduced risk of LDHIV severity [P = 0.07, odds ratio (OR) = 0.55]. MTP -493T allele exhibited a non-significantly reduced risk for the development of dyslipidemia (P = 0.08, OR = 0.71). In patients with HIVLD, the ABCG2 34GA genotype was linked with impaired low-density lipoprotein levels and showed a reduced risk for LDHIV severity (P = 0.04, OR = 0.17). In patients without HIVLD, the ABCG2 34GA genotype was associated with impaired triglyceride levels with marginal significance and showed an increased risk for the development of dyslipidemia (P = 0.07, OR = 2.76). The expression level of MTP gene was 1.22-fold decreased in patients without HIVLD compared with that in patients with HIVLD. ABCG2 gene was upregulated 2.16-fold in patients with HIVLD than in patients without HIVLD. In conclusion, MTP -493C/T polymorphism influences the expression level of MTP in patients without HIVLD. Individuals without HIVLD having ABCG2 34GA genotype with impaired triglyceride levels may facilitate dyslipidemia risk.
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spelling pubmed-102620452023-06-15 Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients Singh, HariOm Jori, Chandrashekhar Shyamveer Mahajan, Supriya D. Aalinkeel, Ravikumar Kaliyappan, Kathiravan Schwartz, Stanley A. Bhattacharya, Meenakshi Shaikh, Ruhi Salve, Madhukar Deshmukh, Jyoti Ali, Nemat Parvez, Mohammad Khalid Front Cardiovasc Med Cardiovascular Medicine HIV-associated lipodystrophy (HIVLD) is a metabolic condition with an irregularity in the production of lipoprotein particles, and its occurrence varies among HIV-infected patients. MTP and ABCG2 genes have a role in the transport of lipoproteins. The polymorphisms of MTP -493G/T and ABCG2 34G/A affect its expression and influence the secretion and transportation of lipoproteins. Hence, we investigated the MTP -493G/T and ABCG2 34G/A polymorphisms in 187 HIV-infected patients (64 with HIVLD and 123 without HIVLD) along with 139 healthy controls using polymerase chain reaction (PCR)-restriction fragment length polymorphism and expression analysis using real-time PCR. ABCG2 34A allele showed an insignificantly reduced risk of LDHIV severity [P = 0.07, odds ratio (OR) = 0.55]. MTP -493T allele exhibited a non-significantly reduced risk for the development of dyslipidemia (P = 0.08, OR = 0.71). In patients with HIVLD, the ABCG2 34GA genotype was linked with impaired low-density lipoprotein levels and showed a reduced risk for LDHIV severity (P = 0.04, OR = 0.17). In patients without HIVLD, the ABCG2 34GA genotype was associated with impaired triglyceride levels with marginal significance and showed an increased risk for the development of dyslipidemia (P = 0.07, OR = 2.76). The expression level of MTP gene was 1.22-fold decreased in patients without HIVLD compared with that in patients with HIVLD. ABCG2 gene was upregulated 2.16-fold in patients with HIVLD than in patients without HIVLD. In conclusion, MTP -493C/T polymorphism influences the expression level of MTP in patients without HIVLD. Individuals without HIVLD having ABCG2 34GA genotype with impaired triglyceride levels may facilitate dyslipidemia risk. Frontiers Media S.A. 2023-05-30 /pmc/articles/PMC10262045/ /pubmed/37324630 http://dx.doi.org/10.3389/fcvm.2023.1177054 Text en © 2023 Singh, Jori, Shyamveer, Mahajan, Aalinkeel, Kaliyappan, Schwartz, Bhattacharya, Shaikh, Salve, Deshmukh, Ali and Parvez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Singh, HariOm
Jori, Chandrashekhar
Shyamveer
Mahajan, Supriya D.
Aalinkeel, Ravikumar
Kaliyappan, Kathiravan
Schwartz, Stanley A.
Bhattacharya, Meenakshi
Shaikh, Ruhi
Salve, Madhukar
Deshmukh, Jyoti
Ali, Nemat
Parvez, Mohammad Khalid
Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients
title Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients
title_full Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients
title_fullStr Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients
title_full_unstemmed Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients
title_short Comparative analysis of MTP -493G/T and ABCG2 34G/A polymorphisms and theirs expression in HIV-associated lipodystrophy patients
title_sort comparative analysis of mtp -493g/t and abcg2 34g/a polymorphisms and theirs expression in hiv-associated lipodystrophy patients
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262045/
https://www.ncbi.nlm.nih.gov/pubmed/37324630
http://dx.doi.org/10.3389/fcvm.2023.1177054
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