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Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot study

BACKGROUND: Chronic obstructive pulmonary disease (COPD) is one of the world’s leading causes of death and a major chronic respiratory disease. Aerobic exercise, the cornerstone of pulmonary rehabilitation, improves prognosis of COPD patients; however, few studies have comprehensively examined the c...

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Autores principales: Liu, Panpan, Zhang, Meilan, Gao, Hongchang, Han, Shaojun, Liu, Jinming, Sun, Xingguo, Zhao, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262399/
https://www.ncbi.nlm.nih.gov/pubmed/37312153
http://dx.doi.org/10.1186/s12931-023-02461-y
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author Liu, Panpan
Zhang, Meilan
Gao, Hongchang
Han, Shaojun
Liu, Jinming
Sun, Xingguo
Zhao, Lei
author_facet Liu, Panpan
Zhang, Meilan
Gao, Hongchang
Han, Shaojun
Liu, Jinming
Sun, Xingguo
Zhao, Lei
author_sort Liu, Panpan
collection PubMed
description BACKGROUND: Chronic obstructive pulmonary disease (COPD) is one of the world’s leading causes of death and a major chronic respiratory disease. Aerobic exercise, the cornerstone of pulmonary rehabilitation, improves prognosis of COPD patients; however, few studies have comprehensively examined the changes in RNA transcript levels and the crosstalk between various transcripts in this context. This study identified the expression of RNA transcripts in COPD patients who engaged in aerobic exercise training for 12 weeks, and further constructions of the possible RNAs networks were made. METHODS: Peripheral blood samples for all four COPD patients who benefited from 12 weeks of PR were collected pre- and post-aerobic exercises and evaluated for the expression of mRNA, miRNA, lncRNA, and circRNA with high-throughput RNA sequencing followed by GEO date validation. In addition, enrichment analyses were conducted on different expressed mRNAs. LncRNA-mRNA and circRNA-mRNA coexpression networks, as well as lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA competing expression networks (ceRNAs) in COPD were constructed. RESULTS: We identified and analyzed the differentially expressed mRNAs and noncoding RNAs in the peripheral blood of COPD patients’ post-exercise. Eighty-six mRNAs, 570 lncRNAs, 8 miRNAs, and 2087 circRNAs were differentially expressed. Direct function enrichment analysis and Gene Set Variation Analysis showed that differentially expressed RNAs(DE-RNAs) correlated with several critical biological processes such as chemotaxis, DNA replication, anti-infection humoral response, oxidative phosphorylation, and immunometabolism, which might affect the progression of COPD. Some DE-RNAs were validated by Geo databases and RT-PCR, and the results were highly correlated with RNA sequencing. We constructed ceRNA networks of DE-RNAs in COPD. CONCLUSIONS: The systematic understanding of the impact of aerobic exercise on COPD was achieved using transcriptomic profiling. This research offers a number of potential candidates for clarifying the regulatory mechanisms that exercise has on COPD, which could ultimately help in understanding the pathophysiology of COPD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-023-02461-y.
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spelling pubmed-102623992023-06-15 Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot study Liu, Panpan Zhang, Meilan Gao, Hongchang Han, Shaojun Liu, Jinming Sun, Xingguo Zhao, Lei Respir Res Research BACKGROUND: Chronic obstructive pulmonary disease (COPD) is one of the world’s leading causes of death and a major chronic respiratory disease. Aerobic exercise, the cornerstone of pulmonary rehabilitation, improves prognosis of COPD patients; however, few studies have comprehensively examined the changes in RNA transcript levels and the crosstalk between various transcripts in this context. This study identified the expression of RNA transcripts in COPD patients who engaged in aerobic exercise training for 12 weeks, and further constructions of the possible RNAs networks were made. METHODS: Peripheral blood samples for all four COPD patients who benefited from 12 weeks of PR were collected pre- and post-aerobic exercises and evaluated for the expression of mRNA, miRNA, lncRNA, and circRNA with high-throughput RNA sequencing followed by GEO date validation. In addition, enrichment analyses were conducted on different expressed mRNAs. LncRNA-mRNA and circRNA-mRNA coexpression networks, as well as lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA competing expression networks (ceRNAs) in COPD were constructed. RESULTS: We identified and analyzed the differentially expressed mRNAs and noncoding RNAs in the peripheral blood of COPD patients’ post-exercise. Eighty-six mRNAs, 570 lncRNAs, 8 miRNAs, and 2087 circRNAs were differentially expressed. Direct function enrichment analysis and Gene Set Variation Analysis showed that differentially expressed RNAs(DE-RNAs) correlated with several critical biological processes such as chemotaxis, DNA replication, anti-infection humoral response, oxidative phosphorylation, and immunometabolism, which might affect the progression of COPD. Some DE-RNAs were validated by Geo databases and RT-PCR, and the results were highly correlated with RNA sequencing. We constructed ceRNA networks of DE-RNAs in COPD. CONCLUSIONS: The systematic understanding of the impact of aerobic exercise on COPD was achieved using transcriptomic profiling. This research offers a number of potential candidates for clarifying the regulatory mechanisms that exercise has on COPD, which could ultimately help in understanding the pathophysiology of COPD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-023-02461-y. BioMed Central 2023-06-13 2023 /pmc/articles/PMC10262399/ /pubmed/37312153 http://dx.doi.org/10.1186/s12931-023-02461-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Panpan
Zhang, Meilan
Gao, Hongchang
Han, Shaojun
Liu, Jinming
Sun, Xingguo
Zhao, Lei
Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot study
title Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot study
title_full Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot study
title_fullStr Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot study
title_full_unstemmed Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot study
title_short Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot study
title_sort regulation of whole-transcriptome sequencing expression in copd after personalized precise exercise training: a pilot study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262399/
https://www.ncbi.nlm.nih.gov/pubmed/37312153
http://dx.doi.org/10.1186/s12931-023-02461-y
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