Cargando…
Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd
BACKGROUND: Host-associated microbes are major determinants of the host phenotypes. In the present study, we used dairy cows with different scores of susceptibility to mastitis with the aim to explore the relationships between microbiota composition and different factors in various body sites throug...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262541/ https://www.ncbi.nlm.nih.gov/pubmed/37308970 http://dx.doi.org/10.1186/s42523-023-00252-w |
_version_ | 1785058074250182656 |
---|---|
author | Mariadassou, Mahendra Nouvel, Laurent X. Constant, Fabienne Morgavi, Diego P. Rault, Lucie Barbey, Sarah Helloin, Emmanuelle Rué, Olivier Schbath, Sophie Launay, Frederic Sandra, Olivier Lefebvre, Rachel Le Loir, Yves Germon, Pierre Citti, Christine Even, Sergine |
author_facet | Mariadassou, Mahendra Nouvel, Laurent X. Constant, Fabienne Morgavi, Diego P. Rault, Lucie Barbey, Sarah Helloin, Emmanuelle Rué, Olivier Schbath, Sophie Launay, Frederic Sandra, Olivier Lefebvre, Rachel Le Loir, Yves Germon, Pierre Citti, Christine Even, Sergine |
author_sort | Mariadassou, Mahendra |
collection | PubMed |
description | BACKGROUND: Host-associated microbes are major determinants of the host phenotypes. In the present study, we used dairy cows with different scores of susceptibility to mastitis with the aim to explore the relationships between microbiota composition and different factors in various body sites throughout lactation as well as the intra- and inter-animal microbial sharing. RESULTS: Microbiotas from the mouth, nose, vagina and milk of 45 lactating dairy cows were characterized by metataxonomics at four time points during the first lactation, from 1-week pre-partum to 7 months post-partum. Each site harbored a specific community that changed with time, likely reflecting physiological changes in the transition period and changes in diet and housing. Importantly, we found a significant number of microbes shared among different anatomical sites within each animal. This was between nearby anatomic sites, with up to 32% of the total number of Amplicon Sequence Variants (ASVs) of the oral microbiota shared with the nasal microbiota but also between distant ones (e.g. milk with nasal and vaginal microbiotas). In contrast, the share of microbes between animals was limited (< 7% of ASVs shared by more than 50% of the herd for a given site and time point). The latter widely shared ASVs were mainly found in the oral and nasal microbiotas. These results thus indicate that despite a common environment and diet, each animal hosted a specific set of bacteria, supporting a tight interplay between each animal and its microbiota. The score of susceptibility to mastitis was slightly but significantly related to the microbiota associated to milk suggesting a link between host genetics and microbiota. CONCLUSIONS: This work highlights an important sharing of microbes between relevant microbiotas involved in health and production at the animal level, whereas the presence of common microbes was limited between animals of the herd. This suggests a host regulation of body-associated microbiotas that seems to be differently expressed depending on the body site, as suggested by changes in the milk microbiota that were associated to genotypes of susceptibility to mastitis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-023-00252-w. |
format | Online Article Text |
id | pubmed-10262541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-102625412023-06-15 Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd Mariadassou, Mahendra Nouvel, Laurent X. Constant, Fabienne Morgavi, Diego P. Rault, Lucie Barbey, Sarah Helloin, Emmanuelle Rué, Olivier Schbath, Sophie Launay, Frederic Sandra, Olivier Lefebvre, Rachel Le Loir, Yves Germon, Pierre Citti, Christine Even, Sergine Anim Microbiome Research BACKGROUND: Host-associated microbes are major determinants of the host phenotypes. In the present study, we used dairy cows with different scores of susceptibility to mastitis with the aim to explore the relationships between microbiota composition and different factors in various body sites throughout lactation as well as the intra- and inter-animal microbial sharing. RESULTS: Microbiotas from the mouth, nose, vagina and milk of 45 lactating dairy cows were characterized by metataxonomics at four time points during the first lactation, from 1-week pre-partum to 7 months post-partum. Each site harbored a specific community that changed with time, likely reflecting physiological changes in the transition period and changes in diet and housing. Importantly, we found a significant number of microbes shared among different anatomical sites within each animal. This was between nearby anatomic sites, with up to 32% of the total number of Amplicon Sequence Variants (ASVs) of the oral microbiota shared with the nasal microbiota but also between distant ones (e.g. milk with nasal and vaginal microbiotas). In contrast, the share of microbes between animals was limited (< 7% of ASVs shared by more than 50% of the herd for a given site and time point). The latter widely shared ASVs were mainly found in the oral and nasal microbiotas. These results thus indicate that despite a common environment and diet, each animal hosted a specific set of bacteria, supporting a tight interplay between each animal and its microbiota. The score of susceptibility to mastitis was slightly but significantly related to the microbiota associated to milk suggesting a link between host genetics and microbiota. CONCLUSIONS: This work highlights an important sharing of microbes between relevant microbiotas involved in health and production at the animal level, whereas the presence of common microbes was limited between animals of the herd. This suggests a host regulation of body-associated microbiotas that seems to be differently expressed depending on the body site, as suggested by changes in the milk microbiota that were associated to genotypes of susceptibility to mastitis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-023-00252-w. BioMed Central 2023-06-12 /pmc/articles/PMC10262541/ /pubmed/37308970 http://dx.doi.org/10.1186/s42523-023-00252-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Mariadassou, Mahendra Nouvel, Laurent X. Constant, Fabienne Morgavi, Diego P. Rault, Lucie Barbey, Sarah Helloin, Emmanuelle Rué, Olivier Schbath, Sophie Launay, Frederic Sandra, Olivier Lefebvre, Rachel Le Loir, Yves Germon, Pierre Citti, Christine Even, Sergine Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd |
title | Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd |
title_full | Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd |
title_fullStr | Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd |
title_full_unstemmed | Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd |
title_short | Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd |
title_sort | microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10262541/ https://www.ncbi.nlm.nih.gov/pubmed/37308970 http://dx.doi.org/10.1186/s42523-023-00252-w |
work_keys_str_mv | AT mariadassoumahendra microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT nouvellaurentx microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT constantfabienne microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT morgavidiegop microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT raultlucie microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT barbeysarah microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT helloinemmanuelle microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT rueolivier microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT schbathsophie microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT launayfrederic microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT sandraolivier microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT lefebvrerachel microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT leloiryves microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT germonpierre microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT cittichristine microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd AT evensergine microbiotamembersfrombodysitesofdairycowsarelargelysharedwithinindividualhoststhroughoutlactationbutsharingislimitedintheherd |