Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates

Bluetongue virus (BTV) causes bluetongue disease in ruminants and sheep. The current live attenuated and inactivated vaccines available for prevention pose several risks, and there is thus a need for vaccines that are safer, economically viable, and effective against multiple circulating serotypes....

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Autores principales: Gwynn, A., Mbewana, S., Lubisi, B. A., Tshabalala, H. M., Rybicki, E. P., Meyers, A. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2023
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264491/
https://www.ncbi.nlm.nih.gov/pubmed/37310539
http://dx.doi.org/10.1007/s00705-023-05790-x
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author Gwynn, A.
Mbewana, S.
Lubisi, B. A.
Tshabalala, H. M.
Rybicki, E. P.
Meyers, A. E.
author_facet Gwynn, A.
Mbewana, S.
Lubisi, B. A.
Tshabalala, H. M.
Rybicki, E. P.
Meyers, A. E.
author_sort Gwynn, A.
collection PubMed
description Bluetongue virus (BTV) causes bluetongue disease in ruminants and sheep. The current live attenuated and inactivated vaccines available for prevention pose several risks, and there is thus a need for vaccines that are safer, economically viable, and effective against multiple circulating serotypes. This work describes the development of recombinant virus-like particle (VLP) vaccine candidates in plants, which are assembled by co-expression of the four BTV serotype 8 major structural proteins. We show that substitution of a neutralising tip domain of BTV8 VP2 with that of BTV1 VP2 resulted in the assembly of VLPs that stimulated serotype-specific antibodies as well as virus-specific neutralising antibodies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05790-x.
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spelling pubmed-102644912023-06-15 Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates Gwynn, A. Mbewana, S. Lubisi, B. A. Tshabalala, H. M. Rybicki, E. P. Meyers, A. E. Arch Virol Brief Report Bluetongue virus (BTV) causes bluetongue disease in ruminants and sheep. The current live attenuated and inactivated vaccines available for prevention pose several risks, and there is thus a need for vaccines that are safer, economically viable, and effective against multiple circulating serotypes. This work describes the development of recombinant virus-like particle (VLP) vaccine candidates in plants, which are assembled by co-expression of the four BTV serotype 8 major structural proteins. We show that substitution of a neutralising tip domain of BTV8 VP2 with that of BTV1 VP2 resulted in the assembly of VLPs that stimulated serotype-specific antibodies as well as virus-specific neutralising antibodies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05790-x. Springer Vienna 2023-06-13 2023 /pmc/articles/PMC10264491/ /pubmed/37310539 http://dx.doi.org/10.1007/s00705-023-05790-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Report
Gwynn, A.
Mbewana, S.
Lubisi, B. A.
Tshabalala, H. M.
Rybicki, E. P.
Meyers, A. E.
Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates
title Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates
title_full Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates
title_fullStr Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates
title_full_unstemmed Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates
title_short Chimaeric plant-produced bluetongue virus particles as potential vaccine candidates
title_sort chimaeric plant-produced bluetongue virus particles as potential vaccine candidates
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10264491/
https://www.ncbi.nlm.nih.gov/pubmed/37310539
http://dx.doi.org/10.1007/s00705-023-05790-x
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